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The large fraction of heterochromatin in Drosophila neurons is bound by both B-type lamin and HP1a. / Pindyurin, Alexey V.; Ilyin, Artem A.; Ivankin, Anton V. et al.
In: Epigenetics & chromatin, Vol. 11, No. 1, 65, 01.11.2018, p. 65.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - The large fraction of heterochromatin in Drosophila neurons is bound by both B-type lamin and HP1a
AU - Pindyurin, Alexey V.
AU - Ilyin, Artem A.
AU - Ivankin, Anton V.
AU - Tselebrovsky, Mikhail V.
AU - Nenasheva, Valentina V.
AU - Mikhaleva, Elena A.
AU - Pagie, Ludo
AU - van Steensel, Bas
AU - Shevelyov, Yuri Y.
N1 - Publisher Copyright: © 2018 The Author(s).
PY - 2018/11/1
Y1 - 2018/11/1
N2 - BACKGROUND: In most mammalian cell lines, chromatin located at the nuclear periphery is represented by condensed heterochromatin, as evidenced by microscopy observations and DamID mapping of lamina-associated domains (LADs) enriched in dimethylated Lys9 of histone H3 (H3K9me2). However, in Kc167 cell culture, the only Drosophilla cell type where LADs have previously been mapped, they are neither H3K9me2-enriched nor overlapped with the domains of heterochromatin protein 1a (HP1a). RESULTS: Here, using cell type-specific DamID we mapped genome-wide LADs, HP1a and Polycomb (Pc) domains from the central brain, Repo-positive glia, Elav-positive neurons and the fat body of Drosophila third instar larvae. Strikingly, contrary to Kc167 cells of embryonic origin, in neurons and, to a lesser extent, in glia and the fat body, HP1a domains appear to overlap strongly with LADs in both the chromosome arms and pericentromeric regions. Accordingly, centromeres reside closer to the nuclear lamina in neurons than in Kc167 cells. As expected, active gene promoters are mostly not present in LADs, HP1a and Pc domains. These domains are occupied by silent or weakly expressed genes with genes residing in the HP1a-bound LADs expressed at the lowest level. CONCLUSIONS: In various differentiated Drosophila cell types, we discovered the existence of peripheral heterochromatin, similar to that observed in mammals. Our findings support the model that peripheral heterochromatin matures enhancing the repression of unwanted genes as cells terminally differentiate.
AB - BACKGROUND: In most mammalian cell lines, chromatin located at the nuclear periphery is represented by condensed heterochromatin, as evidenced by microscopy observations and DamID mapping of lamina-associated domains (LADs) enriched in dimethylated Lys9 of histone H3 (H3K9me2). However, in Kc167 cell culture, the only Drosophilla cell type where LADs have previously been mapped, they are neither H3K9me2-enriched nor overlapped with the domains of heterochromatin protein 1a (HP1a). RESULTS: Here, using cell type-specific DamID we mapped genome-wide LADs, HP1a and Polycomb (Pc) domains from the central brain, Repo-positive glia, Elav-positive neurons and the fat body of Drosophila third instar larvae. Strikingly, contrary to Kc167 cells of embryonic origin, in neurons and, to a lesser extent, in glia and the fat body, HP1a domains appear to overlap strongly with LADs in both the chromosome arms and pericentromeric regions. Accordingly, centromeres reside closer to the nuclear lamina in neurons than in Kc167 cells. As expected, active gene promoters are mostly not present in LADs, HP1a and Pc domains. These domains are occupied by silent or weakly expressed genes with genes residing in the HP1a-bound LADs expressed at the lowest level. CONCLUSIONS: In various differentiated Drosophila cell types, we discovered the existence of peripheral heterochromatin, similar to that observed in mammals. Our findings support the model that peripheral heterochromatin matures enhancing the repression of unwanted genes as cells terminally differentiate.
KW - B-type lamin
KW - Drosophila
KW - Heterochromatin
KW - HP1
KW - Lamina-associated domains
KW - Polycomb
KW - Cell Line
KW - Heterochromatin/genetics
KW - Drosophila Proteins/genetics
KW - Chromosomal Proteins, Non-Histone/genetics
KW - Histones/metabolism
KW - Animals
KW - Neurons/metabolism
KW - Protein Binding
KW - Lamin Type B/genetics
KW - CHROMATIN-STRUCTURE
KW - PROTEIN-1 HP1
KW - HISTONE H3
KW - GENE-EXPRESSION
KW - LYSINE 9
KW - STEM-CELLS
KW - NUCLEAR LAMINA
KW - GENOME
KW - BINDING
KW - MELANOGASTER
UR - http://www.scopus.com/inward/record.url?scp=85055915604&partnerID=8YFLogxK
U2 - 10.1186/s13072-018-0235-8
DO - 10.1186/s13072-018-0235-8
M3 - Article
C2 - 30384843
AN - SCOPUS:85055915604
VL - 11
SP - 65
JO - Epigenetics and Chromatin
JF - Epigenetics and Chromatin
SN - 1756-8935
IS - 1
M1 - 65
ER -
ID: 17303354