Research output: Contribution to journal › Article › peer-review
The isolation of influenza a virus from plumage of waterfowl during autumn migration. / Gulyaeva, Marina A.; Sharshov, Kirill A.; Sobolev, Ivan A. et al.
In: Юг России: экология, развитие, Vol. 13, No. 3, 01.01.2018, p. 134-141.Research output: Contribution to journal › Article › peer-review
}
TY - JOUR
T1 - The isolation of influenza a virus from plumage of waterfowl during autumn migration
AU - Gulyaeva, Marina A.
AU - Sharshov, Kirill A.
AU - Sobolev, Ivan A.
AU - Yurlov, Alexander K.
AU - Gadzhiev, Alimurad A.
AU - Rabazanov, Nukhkadi I.
AU - Shestopalova, Lidia V.
AU - Shestopalov, Alexander M.
PY - 2018/1/1
Y1 - 2018/1/1
N2 - Aim. In the present work we investigated the circulation of AIV in wild bird populations and studied the sorption of the influenza virus in the feathers of wild waterfowl nesting on reservoirs during the autumn mass migration. Material and methods. Sampling was carried out on the territory of the Novosibirsk region on Lake Chany during the period from August to September 2014-2016. Biological samples were collected from 188 wild waterfowl of various species. AIV isolation from cloacal swabs and swabs collected from feathers was carried out in the developing chick embryo system (RCC) as previously recommended. The isolated viruses were tested by HA/HI with specific sera, PCR analysis was carried out with subtyping primers. The genomes of the isolated viruses were sequenced for phylogenetic analysis. Results and discussion. As a result of monitoring, cloacal and feather swabs were collected from 188 individuals belonging to 13 species of the Anseriformes and Charadriiformes, whose representatives are the main natural reservoir of AIV. Fifteen new AI viruses were isolated from the collected samples. Four of them were isolated from plumage samples and the rate was more than 2 times lower, compared with virus isolation from cloacal swabs. Main conclusions. Thus, it can be assumed that avian influenza virus transmission by plumage during migration is not sufficiently taken into account. The key role in AIV ecology may play the virus spreading by its adsorption on bird feathers.
AB - Aim. In the present work we investigated the circulation of AIV in wild bird populations and studied the sorption of the influenza virus in the feathers of wild waterfowl nesting on reservoirs during the autumn mass migration. Material and methods. Sampling was carried out on the territory of the Novosibirsk region on Lake Chany during the period from August to September 2014-2016. Biological samples were collected from 188 wild waterfowl of various species. AIV isolation from cloacal swabs and swabs collected from feathers was carried out in the developing chick embryo system (RCC) as previously recommended. The isolated viruses were tested by HA/HI with specific sera, PCR analysis was carried out with subtyping primers. The genomes of the isolated viruses were sequenced for phylogenetic analysis. Results and discussion. As a result of monitoring, cloacal and feather swabs were collected from 188 individuals belonging to 13 species of the Anseriformes and Charadriiformes, whose representatives are the main natural reservoir of AIV. Fifteen new AI viruses were isolated from the collected samples. Four of them were isolated from plumage samples and the rate was more than 2 times lower, compared with virus isolation from cloacal swabs. Main conclusions. Thus, it can be assumed that avian influenza virus transmission by plumage during migration is not sufficiently taken into account. The key role in AIV ecology may play the virus spreading by its adsorption on bird feathers.
KW - Avian influenza virus
KW - Bird ecology
KW - Plumage
KW - Ways of virus transmission
KW - plumage
KW - avian influenza virus
KW - bird ecology
KW - ways of virus transmission
UR - http://www.scopus.com/inward/record.url?scp=85055885785&partnerID=8YFLogxK
UR - https://apps.webofknowledge.com/full_record.do?product=WOS&search_mode=GeneralSearch&qid=60&SID=F4q8HL5cWl26gLFxjdM
UR - https://www.elibrary.ru/item.asp?id=36276624
U2 - 10.18470/1992-1098-2018-3-134-141
DO - 10.18470/1992-1098-2018-3-134-141
M3 - Article
AN - SCOPUS:85055885785
VL - 13
SP - 134
EP - 141
JO - Юг России: экология, развитие
JF - Юг России: экология, развитие
SN - 1992-1098
IS - 3
ER -
ID: 17488639