Research output: Contribution to journal › Article › peer-review
The in‐silico development of dna markers for breeding of spring barley varieties that are resistant to spot blotch in russia. / Rozanova, Irina V.; Lashina, Nina M.; Efimov, Vadim M. et al.
In: Agriculture (Switzerland), Vol. 10, No. 11, 505, 11.2020, p. 1-16.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - The in‐silico development of dna markers for breeding of spring barley varieties that are resistant to spot blotch in russia
AU - Rozanova, Irina V.
AU - Lashina, Nina M.
AU - Efimov, Vadim M.
AU - Afanasenko, Olga S.
AU - Khlestkina, Elena K.
PY - 2020/11
Y1 - 2020/11
N2 - The fungal pathogen Cochliobolus sativus Drechs. Ex Dastur, anamorph Bipolaris sorokiniana (Sacc.) Shoemaker is one of the most common barley pathogens worldwide and causes spot blotch and root rot in barley. Spot blotch is considered to be the major biotic stress hampering the commercial production of barley. During high disease severity, which occurs in the northwestern region of Russia once every three to four years, yield losses for barley may reach 40%. An increase in common root rot severity results in yield losses that can reach 80%. The goal of the current study was to identify significant markers that can be employed as diagnostic DNA markers to breed C. sativus pathogen‐resistant varieties of barley. In 94 spring barley cultivars and lines, the resistance of seedlings and adult plants to the impact of C. sativus on their leaves and roots was investigated. Five genomic regions associated with resistance to Spot blotch were identified (on chromosome 1H (50–61.2 cM), 2H (68.7–69.68 cM), 3H (18.72–26.18 cM), 7H (7.52–15.44 cM)). No significant loci were determined to be associated with root rot. According to obtained data, 11 significant SNPs were converted into KASP markers and 6 markers located on chromosome 3H were determined to possess good accuracy and the potential to be employed in marker‐assisted selection.
AB - The fungal pathogen Cochliobolus sativus Drechs. Ex Dastur, anamorph Bipolaris sorokiniana (Sacc.) Shoemaker is one of the most common barley pathogens worldwide and causes spot blotch and root rot in barley. Spot blotch is considered to be the major biotic stress hampering the commercial production of barley. During high disease severity, which occurs in the northwestern region of Russia once every three to four years, yield losses for barley may reach 40%. An increase in common root rot severity results in yield losses that can reach 80%. The goal of the current study was to identify significant markers that can be employed as diagnostic DNA markers to breed C. sativus pathogen‐resistant varieties of barley. In 94 spring barley cultivars and lines, the resistance of seedlings and adult plants to the impact of C. sativus on their leaves and roots was investigated. Five genomic regions associated with resistance to Spot blotch were identified (on chromosome 1H (50–61.2 cM), 2H (68.7–69.68 cM), 3H (18.72–26.18 cM), 7H (7.52–15.44 cM)). No significant loci were determined to be associated with root rot. According to obtained data, 11 significant SNPs were converted into KASP markers and 6 markers located on chromosome 3H were determined to possess good accuracy and the potential to be employed in marker‐assisted selection.
KW - Association analysis
KW - KASP markers
KW - PLS analysis
KW - Root rot
KW - Spot blotch
KW - spot blotch
KW - COMMON ROOT-ROT
KW - COCHLIOBOLUS-SATIVUS
KW - IDENTIFICATION
KW - root rot
KW - association analysis
KW - GENETICS
KW - DISEASE
UR - http://www.scopus.com/inward/record.url?scp=85094608684&partnerID=8YFLogxK
U2 - 10.3390/agriculture10110505
DO - 10.3390/agriculture10110505
M3 - Article
AN - SCOPUS:85094608684
VL - 10
SP - 1
EP - 16
JO - Agriculture (Switzerland)
JF - Agriculture (Switzerland)
SN - 2077-0472
IS - 11
M1 - 505
ER -
ID: 25862616