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The Impact of SNP-Induced Amino Acid Substitutions L19P and G66R in the dRP-Lyase Domain of Human DNA Polymerase β on Enzyme Activities. / Kladova, Olga A.; Tyugashev, Timofey E.; Yakimov, Denis V. et al.

In: International Journal of Molecular Sciences, Vol. 25, No. 8, 4182, 04.2024.

Research output: Contribution to journalArticlepeer-review

Harvard

Kladova, OA, Tyugashev, TE, Yakimov, DV, Mikushina, ES, Novopashina, DS, Kuznetsov, NA & Kuznetsova, AA 2024, 'The Impact of SNP-Induced Amino Acid Substitutions L19P and G66R in the dRP-Lyase Domain of Human DNA Polymerase β on Enzyme Activities', International Journal of Molecular Sciences, vol. 25, no. 8, 4182. https://doi.org/10.3390/ijms25084182

APA

Kladova, O. A., Tyugashev, T. E., Yakimov, D. V., Mikushina, E. S., Novopashina, D. S., Kuznetsov, N. A., & Kuznetsova, A. A. (2024). The Impact of SNP-Induced Amino Acid Substitutions L19P and G66R in the dRP-Lyase Domain of Human DNA Polymerase β on Enzyme Activities. International Journal of Molecular Sciences, 25(8), [4182]. https://doi.org/10.3390/ijms25084182

Vancouver

Kladova OA, Tyugashev TE, Yakimov DV, Mikushina ES, Novopashina DS, Kuznetsov NA et al. The Impact of SNP-Induced Amino Acid Substitutions L19P and G66R in the dRP-Lyase Domain of Human DNA Polymerase β on Enzyme Activities. International Journal of Molecular Sciences. 2024 Apr;25(8):4182. doi: 10.3390/ijms25084182

Author

Kladova, Olga A. ; Tyugashev, Timofey E. ; Yakimov, Denis V. et al. / The Impact of SNP-Induced Amino Acid Substitutions L19P and G66R in the dRP-Lyase Domain of Human DNA Polymerase β on Enzyme Activities. In: International Journal of Molecular Sciences. 2024 ; Vol. 25, No. 8.

BibTeX

@article{0a4b76ac843148f5b17b7f8c8851fccc,
title = "The Impact of SNP-Induced Amino Acid Substitutions L19P and G66R in the dRP-Lyase Domain of Human DNA Polymerase β on Enzyme Activities",
abstract = "Base excision repair (BER), which involves the sequential activity of DNA glycosylases, apurinic/apyrimidinic endonucleases, DNA polymerases, and DNA ligases, is one of the enzymatic systems that preserve the integrity of the genome. Normal BER is effective, but due to single-nucleotide polymorphisms (SNPs), the enzymes themselves—whose main function is to identify and eliminate damaged bases—can undergo amino acid changes. One of the enzymes in BER is DNA polymerase β (Polβ), whose function is to fill gaps in DNA. SNPs can significantly affect the catalytic activity of an enzyme by causing an amino acid substitution. In this work, pre-steady-state kinetic analyses and molecular dynamics simulations were used to examine the activity of naturally occurring variants of Polβ that have the substitutions L19P and G66R in the dRP-lyase domain. Despite the substantial distance between the dRP-lyase domain and the nucleotidyltransferase active site, it was found that the capacity to form a complex with DNA and with an incoming dNTP is significantly altered by these substitutions. Therefore, the lower activity of the tested polymorphic variants may be associated with a greater number of unrepaired DNA lesions.",
keywords = "DNA polymerase beta, DNA repair, enzymatic activity, single-nucleotide polymorphism",
author = "Kladova, {Olga A.} and Tyugashev, {Timofey E.} and Yakimov, {Denis V.} and Mikushina, {Elena S.} and Novopashina, {Daria S.} and Kuznetsov, {Nikita A.} and Kuznetsova, {Aleksandra A.}",
note = "The part of this work involving mutagenesis, enzyme purification, and kinetic analysis was specifically funded by Russian Science Foundation grant no. 21-74-10103. Partial governmental support for equipment use was received from Russian Ministry of Science and Higher Education project no. 121031300041-4.",
year = "2024",
month = apr,
doi = "10.3390/ijms25084182",
language = "English",
volume = "25",
journal = "International Journal of Molecular Sciences",
issn = "1661-6596",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
number = "8",

}

RIS

TY - JOUR

T1 - The Impact of SNP-Induced Amino Acid Substitutions L19P and G66R in the dRP-Lyase Domain of Human DNA Polymerase β on Enzyme Activities

AU - Kladova, Olga A.

AU - Tyugashev, Timofey E.

AU - Yakimov, Denis V.

AU - Mikushina, Elena S.

AU - Novopashina, Daria S.

AU - Kuznetsov, Nikita A.

AU - Kuznetsova, Aleksandra A.

N1 - The part of this work involving mutagenesis, enzyme purification, and kinetic analysis was specifically funded by Russian Science Foundation grant no. 21-74-10103. Partial governmental support for equipment use was received from Russian Ministry of Science and Higher Education project no. 121031300041-4.

PY - 2024/4

Y1 - 2024/4

N2 - Base excision repair (BER), which involves the sequential activity of DNA glycosylases, apurinic/apyrimidinic endonucleases, DNA polymerases, and DNA ligases, is one of the enzymatic systems that preserve the integrity of the genome. Normal BER is effective, but due to single-nucleotide polymorphisms (SNPs), the enzymes themselves—whose main function is to identify and eliminate damaged bases—can undergo amino acid changes. One of the enzymes in BER is DNA polymerase β (Polβ), whose function is to fill gaps in DNA. SNPs can significantly affect the catalytic activity of an enzyme by causing an amino acid substitution. In this work, pre-steady-state kinetic analyses and molecular dynamics simulations were used to examine the activity of naturally occurring variants of Polβ that have the substitutions L19P and G66R in the dRP-lyase domain. Despite the substantial distance between the dRP-lyase domain and the nucleotidyltransferase active site, it was found that the capacity to form a complex with DNA and with an incoming dNTP is significantly altered by these substitutions. Therefore, the lower activity of the tested polymorphic variants may be associated with a greater number of unrepaired DNA lesions.

AB - Base excision repair (BER), which involves the sequential activity of DNA glycosylases, apurinic/apyrimidinic endonucleases, DNA polymerases, and DNA ligases, is one of the enzymatic systems that preserve the integrity of the genome. Normal BER is effective, but due to single-nucleotide polymorphisms (SNPs), the enzymes themselves—whose main function is to identify and eliminate damaged bases—can undergo amino acid changes. One of the enzymes in BER is DNA polymerase β (Polβ), whose function is to fill gaps in DNA. SNPs can significantly affect the catalytic activity of an enzyme by causing an amino acid substitution. In this work, pre-steady-state kinetic analyses and molecular dynamics simulations were used to examine the activity of naturally occurring variants of Polβ that have the substitutions L19P and G66R in the dRP-lyase domain. Despite the substantial distance between the dRP-lyase domain and the nucleotidyltransferase active site, it was found that the capacity to form a complex with DNA and with an incoming dNTP is significantly altered by these substitutions. Therefore, the lower activity of the tested polymorphic variants may be associated with a greater number of unrepaired DNA lesions.

KW - DNA polymerase beta

KW - DNA repair

KW - enzymatic activity

KW - single-nucleotide polymorphism

UR - https://www.scopus.com/record/display.uri?eid=2-s2.0-85191382247&origin=inward&txGid=681cb653fa9eabb14a9dbe8f8a4416ab

UR - https://www.mendeley.com/catalogue/b6d5cabe-4445-3658-ae07-366a888171f4/

U2 - 10.3390/ijms25084182

DO - 10.3390/ijms25084182

M3 - Article

C2 - 38673769

VL - 25

JO - International Journal of Molecular Sciences

JF - International Journal of Molecular Sciences

SN - 1661-6596

IS - 8

M1 - 4182

ER -

ID: 61084811