Research output: Contribution to journal › Article › peer-review
The Disruption of the HIV-1 Gag Start Codon via Editing Using MmCas12m-Dual Base Editor-Loaded Virus-like Particles. / Aliev, Timur; Imatdinov, Almaz; Prudnikova, Elena et al.
In: Current issues in molecular biology, Vol. 48, No. 3, 241, 25.02.2026.Research output: Contribution to journal › Article › peer-review
}
TY - JOUR
T1 - The Disruption of the HIV-1 Gag Start Codon via Editing Using MmCas12m-Dual Base Editor-Loaded Virus-like Particles
AU - Aliev, Timur
AU - Imatdinov, Almaz
AU - Prudnikova, Elena
AU - Taranov, Oleg
AU - Emtsova, Ksenia
AU - Imatdinov, Ilnaz
AU - Agafonov, Alexander
N1 - The Disruption of the HIV-1 Gag Start Codon via Editing Using MmCas12m-Dual Base Editor-Loaded Virus-like Particles / T. Aliev, A. Imatdinov, E. Prudnikova [et al.] // Current Issues in Molecular Biology. – 2026. – Vol. 48, No. 3. – P. 241. – DOI 10.3390/cimb48030241. – EDN ODNMJT. This research was funded by the Ministry of Science and Higher Education of the Russian Federation (The Federal Scientific-technical programme for genetic technologies development for 2019–2030, agreement No. 075-15-2025-526).
PY - 2026/2/25
Y1 - 2026/2/25
N2 - Approaches to delivering gene editing tools in the form of ribonucleoproteins may provide a safety advantage over the delivery of nucleic acids encoding ribonucleoproteins. Virus-based vectors are widely used as a delivery platform. However, the persistence of viral exogenous nucleic acids can cause increased genotoxicity. Virus-like particles (VLPs) do not contain an expression cassette and can act as a platform for the delivery of ready-made ribonucleoprotein complexes. The absence of nucleic acids in VLPs eliminates the risk of insertional mutagenesis compared to widely used lentiviruses or adeno-associated viruses. Therefore, we used VLPs to deliver the ribonucleoprotein complex MmCas12m–TadDE to disrupt the HIV-1 gag gene start codon. We detected VLP morphogenesis using electron microscopy. We confirmed the incorporation of MmCas12m–TadDE into VLPs. We achieved an editing efficiency of about 9% in some cases with minimal off-target effects, which confirms the prospect of using VLPs as a platform for delivering genomic editing tools.
AB - Approaches to delivering gene editing tools in the form of ribonucleoproteins may provide a safety advantage over the delivery of nucleic acids encoding ribonucleoproteins. Virus-based vectors are widely used as a delivery platform. However, the persistence of viral exogenous nucleic acids can cause increased genotoxicity. Virus-like particles (VLPs) do not contain an expression cassette and can act as a platform for the delivery of ready-made ribonucleoprotein complexes. The absence of nucleic acids in VLPs eliminates the risk of insertional mutagenesis compared to widely used lentiviruses or adeno-associated viruses. Therefore, we used VLPs to deliver the ribonucleoprotein complex MmCas12m–TadDE to disrupt the HIV-1 gag gene start codon. We detected VLP morphogenesis using electron microscopy. We confirmed the incorporation of MmCas12m–TadDE into VLPs. We achieved an editing efficiency of about 9% in some cases with minimal off-target effects, which confirms the prospect of using VLPs as a platform for delivering genomic editing tools.
KW - CRISPR/Cas
KW - HIV
KW - MmCas12m
KW - base editing
KW - gene therapy
KW - virus-like particles
KW - ВИЧ
KW - генная терапия
KW - редактирование оснований
KW - CRISPR/Cas
KW - MmCas12m
KW - вирусоподобные частицы
UR - https://www.mendeley.com/catalogue/f907f14c-6fef-36e3-9eba-c0008f3fab2b/
UR - https://www.scopus.com/pages/publications/105033710250
UR - https://elibrary.ru/item.asp?id=89957759
U2 - 10.3390/cimb48030241
DO - 10.3390/cimb48030241
M3 - Article
C2 - 41899394
VL - 48
JO - Current issues in molecular biology
JF - Current issues in molecular biology
SN - 1467-3037
IS - 3
M1 - 241
ER -
ID: 85125650