TY - JOUR

T1 - The development of a liquid chromatography high‐resolution mass spectrometric method for apixaban quantification in dried plasma spots in parallel reaction monitoring mode

AU - Chernonosov, Alexander

AU - Aksenova, Liliya

AU - Koval, Vladimir

N1 - Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland. Copyright: Copyright 2021 Elsevier B.V., All rights reserved.

PY - 2021/3

Y1 - 2021/3

N2 - This work aimed at developing and validating a rapid, sensitive, and robust method of liquid chromatography with high‐resolution mass spectrometry (LC–HRMS) in parallel reaction monitoring (PRM) mode for apixaban quantification in dried plasma spots (DPSs) with a simple extraction procedure. A 25 μL sample of human plasma was placed onto Whatman 903 Protein Saver Cards and allowed to dry; 3.2 mm diameter disks were cut out from DPSs using a puncher, and 100 μL of a working internal standard solution was added to each sample. After this, they were vortexed on a shaker for 15 min at 800 rpm and 40 °C and quick centrifugation (10,000× g, 10 s), and then the extracts were transferred into a 300 μL vial for LC–HRMS. Data were acquired in PRM mode via detection of all target product ions with 10 ppm tolerance. Total analysis time was 5 min. The LC–HRMS method was validated for the 10–400 ng/mL range with R2 > 0.99. Within this range, intra‐ and interday variability of precision and accuracy was <10%, and recovery was 69.7–85.1%. Apixaban was stable after brief storage at room tem-perature, and at 4 °C for up to a month. The method development and validation results proved that this LC–HRMS assay of apixaban in DPSs is selective and robust.

AB - This work aimed at developing and validating a rapid, sensitive, and robust method of liquid chromatography with high‐resolution mass spectrometry (LC–HRMS) in parallel reaction monitoring (PRM) mode for apixaban quantification in dried plasma spots (DPSs) with a simple extraction procedure. A 25 μL sample of human plasma was placed onto Whatman 903 Protein Saver Cards and allowed to dry; 3.2 mm diameter disks were cut out from DPSs using a puncher, and 100 μL of a working internal standard solution was added to each sample. After this, they were vortexed on a shaker for 15 min at 800 rpm and 40 °C and quick centrifugation (10,000× g, 10 s), and then the extracts were transferred into a 300 μL vial for LC–HRMS. Data were acquired in PRM mode via detection of all target product ions with 10 ppm tolerance. Total analysis time was 5 min. The LC–HRMS method was validated for the 10–400 ng/mL range with R2 > 0.99. Within this range, intra‐ and interday variability of precision and accuracy was <10%, and recovery was 69.7–85.1%. Apixaban was stable after brief storage at room tem-perature, and at 4 °C for up to a month. The method development and validation results proved that this LC–HRMS assay of apixaban in DPSs is selective and robust.

KW - Apixaban

KW - DPS

KW - Dried blood spot

KW - Dried plasma spot

KW - High‐resolution mass spectrometry

KW - LC–HRMS

KW - Parallel reaction monitoring

UR - http://www.scopus.com/inward/record.url?scp=85102530418&partnerID=8YFLogxK

U2 - 10.3390/pr9030450

DO - 10.3390/pr9030450

M3 - Article

AN - SCOPUS:85102530418

VL - 9

SP - 1

EP - 12

JO - Processes

JF - Processes

SN - 2227-9717

IS - 3

M1 - 450

ER -