Research output: Contribution to journal › Article › peer-review
The consensus rye microsatellite map with EST-SSRs transferred from wheat. / Vidakovic, D. O.; Perovic, D.; Semilet, T. V. et al.
In: Вавиловский журнал генетики и селекции, Vol. 24, No. 5, 2020, p. 459-464.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - The consensus rye microsatellite map with EST-SSRs transferred from wheat
AU - Vidakovic, D. O.
AU - Perovic, D.
AU - Semilet, T. V.
AU - Börner, A.
AU - Khlestkina, E. K.
N1 - Видакович Д.О., Перович Д., Семилет Т.В., Бернер А., Хлесткина Е.К. Консенсусная микросателлитная карта ржи с интегрированными EST-SSR маркерами пшеницы // Вавиловский журнал генетики и селекции. - 2020. - Т. 24. - № 5. - С. 459-464
PY - 2020
Y1 - 2020
N2 - Microsatellite (SSR) markers with known precise intrachromosomal locations are widely used for mapping genes in rye and for the investigation of wheat-rye translocation lines and triticale highly demanded for mapping economically important genes and QTL-analysis. One of the sources of novel SSR markers in rye are microsatellites transferable from the wheat genome. Broadening the list of available SSRs in rye mapped to chromosomes is still needed, since some rye chromosome maps still have just a few microsatellite loci mapped. The goal of the current study was to integrate wheat EST-SSRs into the existing rye genetic maps and to construct a consensus rye microsatellite map. Four rye mapping populations (P87/P105, N6/N2, N7/N2 and N7/N6) were tested with CFE (EST-SSRs) primers. A total of 23 Xcfe loci were mapped on rye chromosomes: Xcfe023, -136 and -266 on chromosome 1R, Xcfe006, -067, -175 and -187 on 2R, Xcfe029 and -282 on 3R, Xcfe004, -100, -152, -224 and -260 on 4R, Xcfe037, -208 and -270 on 5R, Xcfe124, -159 and -277 on 6R, Xcfe010, -143 and -228 on 7R. With the exception of Xcfe159 and Xcfe224, all the Xcfe loci mapped were found in orthologous positions considering multiple evolutionary translocations in the rye genome relative to those of common wheat. The consensus map was constructed using mapping data from the four bi-parental populations. It contains a total of 123 microsatellites, 12 SNPs, 118 RFLPs and 2 isozyme loci.
AB - Microsatellite (SSR) markers with known precise intrachromosomal locations are widely used for mapping genes in rye and for the investigation of wheat-rye translocation lines and triticale highly demanded for mapping economically important genes and QTL-analysis. One of the sources of novel SSR markers in rye are microsatellites transferable from the wheat genome. Broadening the list of available SSRs in rye mapped to chromosomes is still needed, since some rye chromosome maps still have just a few microsatellite loci mapped. The goal of the current study was to integrate wheat EST-SSRs into the existing rye genetic maps and to construct a consensus rye microsatellite map. Four rye mapping populations (P87/P105, N6/N2, N7/N2 and N7/N6) were tested with CFE (EST-SSRs) primers. A total of 23 Xcfe loci were mapped on rye chromosomes: Xcfe023, -136 and -266 on chromosome 1R, Xcfe006, -067, -175 and -187 on 2R, Xcfe029 and -282 on 3R, Xcfe004, -100, -152, -224 and -260 on 4R, Xcfe037, -208 and -270 on 5R, Xcfe124, -159 and -277 on 6R, Xcfe010, -143 and -228 on 7R. With the exception of Xcfe159 and Xcfe224, all the Xcfe loci mapped were found in orthologous positions considering multiple evolutionary translocations in the rye genome relative to those of common wheat. The consensus map was constructed using mapping data from the four bi-parental populations. It contains a total of 123 microsatellites, 12 SNPs, 118 RFLPs and 2 isozyme loci.
KW - Genetic mapping
KW - Microsatellite markers
KW - Secale cereale
KW - SSR
KW - Triticum aestivum
KW - Secale cereale
KW - SSR
KW - Triticum aestivum
KW - microsatellite markers
KW - genetic mapping
KW - SECALE-CEREALE L.
KW - GENETIC-MAP
KW - HOMOLOGOUS SYNAPSIS
KW - ALUMINUM TOLERANCE
KW - MEIOTIC GENES
KW - LOCALIZATION
KW - ISOZYME
KW - TRANSLOCATIONS
KW - IDENTIFICATION
KW - SEQUENCE
UR - http://www.scopus.com/inward/record.url?scp=85092028046&partnerID=8YFLogxK
UR - https://www.elibrary.ru/item.asp?id=43842533
U2 - 10.18699/VJ20.48-O
DO - 10.18699/VJ20.48-O
M3 - Article
C2 - 33659829
AN - SCOPUS:85092028046
VL - 24
SP - 459
EP - 464
JO - Вавиловский журнал генетики и селекции
JF - Вавиловский журнал генетики и селекции
SN - 2500-0462
IS - 5
ER -
ID: 25679146