Research output: Contribution to journal › Article › peer-review
The case of X and Y localization of nucleolus organizer regions (NORs) in tragulus javanicus (Cetartiodactyla, Mammalia). / Proskuryakova, Anastasia A.; Kulemzina, Anastasia I.; Perelman, Polina L. et al.
In: Genes, Vol. 9, No. 6, 312, 20.06.2018.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - The case of X and Y localization of nucleolus organizer regions (NORs) in tragulus javanicus (Cetartiodactyla, Mammalia)
AU - Proskuryakova, Anastasia A.
AU - Kulemzina, Anastasia I.
AU - Perelman, Polina L.
AU - Serdukova, Natalia A.
AU - Ryder, Oliver A.
AU - Graphodatsky, Alexander S.
N1 - Publisher Copyright: © 2018 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2018/6/20
Y1 - 2018/6/20
N2 - There are differences in number and localization of nucleolus organizer regions (NORs) in genomes. In mammalian genomes, NORs are located on autosomes, which are often situated on short arms of acrocentric chromosomes and more rarely in telomeric, pericentromeric, or interstitial regions. In this work, we report the unique case of active NORs located on gonоsomes of a eutherian mammal, the Javan mouse-deer (Tragulus javanicus). We have investigated the position of NORs by FISH experiments with ribosomal DNA (rDNA) sequences (18S, 5.8S, and 28S) and show the presence of a single NOR site on the X and Y chromosomes. The NOR is localized interstitially on the p-arm of the X chromosome in close proximity with prominent C-positive heterochromatin blocks and in the pericentromeric area of mostly heterochromatic Y. The NOR sites are active on both the X and Y chromosomes in the studied individual and surrounded by GC enriched heterochromatin. We hypothesize that the surrounding heterochromatin might have played a role in the transfer of NORs from autosomes to sex chromosomes during the karyotype evolution of the Javan mouse-deer.
AB - There are differences in number and localization of nucleolus organizer regions (NORs) in genomes. In mammalian genomes, NORs are located on autosomes, which are often situated on short arms of acrocentric chromosomes and more rarely in telomeric, pericentromeric, or interstitial regions. In this work, we report the unique case of active NORs located on gonоsomes of a eutherian mammal, the Javan mouse-deer (Tragulus javanicus). We have investigated the position of NORs by FISH experiments with ribosomal DNA (rDNA) sequences (18S, 5.8S, and 28S) and show the presence of a single NOR site on the X and Y chromosomes. The NOR is localized interstitially on the p-arm of the X chromosome in close proximity with prominent C-positive heterochromatin blocks and in the pericentromeric area of mostly heterochromatic Y. The NOR sites are active on both the X and Y chromosomes in the studied individual and surrounded by GC enriched heterochromatin. We hypothesize that the surrounding heterochromatin might have played a role in the transfer of NORs from autosomes to sex chromosomes during the karyotype evolution of the Javan mouse-deer.
KW - Chevrotain
KW - Chromosome evolution
KW - Genome
KW - Karyotype
KW - Ribosomal DNA
KW - Sex chromosomes
KW - LOCATION
KW - chevrotain
KW - RECOMBINATION
KW - CHARACIFORMES
KW - ribosomal DNA
KW - chromosome evolution
KW - sex chromosomes
KW - RIBOSOMAL-RNA GENES
KW - PRIMATES
KW - CHROMOSOME EVOLUTION
KW - genome
KW - karyotype
KW - DNA
KW - ARTIODACTYLA
KW - DYNAMICS
KW - KARYOTYPIC ANALYSIS
UR - http://www.scopus.com/inward/record.url?scp=85048811302&partnerID=8YFLogxK
U2 - 10.3390/genes9060312
DO - 10.3390/genes9060312
M3 - Article
C2 - 29925822
AN - SCOPUS:85048811302
VL - 9
JO - Genes
JF - Genes
SN - 2073-4425
IS - 6
M1 - 312
ER -
ID: 14103078