The Blood of the HIV-Infected Patients Contains κ-IgG, λ-IgG, and Bispecific κλ-IgG, Which Possess DNase and Amylolytic Activity

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The Blood of the HIV-Infected Patients Contains κ-IgG, λ-IgG, and Bispecific κλ-IgG, Which Possess DNase and Amylolytic Activity. / Timofeeva, Anna; Sedykh, Sergey; Maksimenko, Lada et al.

In: Life, Vol. 12, No. 2, 304, 02.2022.

Research output: Contribution to journal › Article › peer-review

BibTeX

@article{29e816aef6d847759c49ee7d157d42dd,

title = "The Blood of the HIV-Infected Patients Contains κ-IgG, λ-IgG, and Bispecific κλ-IgG, Which Possess DNase and Amylolytic Activity",

abstract = "Though hundreds of thousands of papers are currently being published on HIV/AIDS, only tens of hundreds of them are devoted to the antibodies generated during the disease. Most of these papers discuss antibodies in HIV/AIDS as a diagnostic tool, and some articles describe neutralizing antibodies as a promising treatment. In this paper, we used affinity chromatography and ELISA to isolate natural IgG from the blood of 26 HIV-infected patients. IgG preparations were separated into the subfractions containing different types of light chains, and catalytic activities of subfractions were analyzed. Here, we show for the first time that the blood of HIV patients contains ~20% of bispecific κλ-IgG, presented with all IgG subclasses. Analysis of DNA-hydrolyzing and amylolytic activity show that most IgG preparations and subfractions are catalytically active. Our results expand the possible biological functions of natural IgG in HIV infection.",

keywords = "Abzymes, Amylolytic activity, Bispecific antibodies, Catalytic activity, DNA hydrolysis, DNAse activity, ELISA, HIV, IgG, Immunoglobulin G",

author = "Anna Timofeeva and Sergey Sedykh and Lada Maksimenko and Tatyana Sedykh and Sergey Skudarnov and Tatyana Ostapova and Svetlana Yaschenko and Natalya Gashnikova and Georgy Nevinsky",

note = "Funding Information: Funding: This research was possible by a grant from the RFBR 20-34-70115 to Sergey Sedykh (isolation and biochemical analysis of antibodies); RSF 21-75-10105 to Anna Timofeeva (analysis of bispecific antibodies); 0245-2021-0009 (121031300041-4) to Georgy Nevinsky (correlation and statistical analysis); and State Research and Development Program 5-21 to Natalya Gashnikova (work with the blood of HIV-infected patients). Publisher Copyright: {\textcopyright} 2022 by the authors. Licensee MDPI, Basel, Switzerland.",

year = "2022",

month = feb,

doi = "10.3390/life12020304",

language = "English",

volume = "12",

journal = "Life",

issn = "2075-1729",

publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",

number = "2",

}

RIS

TY - JOUR

T1 - The Blood of the HIV-Infected Patients Contains κ-IgG, λ-IgG, and Bispecific κλ-IgG, Which Possess DNase and Amylolytic Activity

AU - Timofeeva, Anna

AU - Sedykh, Sergey

AU - Maksimenko, Lada

AU - Sedykh, Tatyana

AU - Skudarnov, Sergey

AU - Ostapova, Tatyana

AU - Yaschenko, Svetlana

AU - Gashnikova, Natalya

AU - Nevinsky, Georgy

N1 - Funding Information: Funding: This research was possible by a grant from the RFBR 20-34-70115 to Sergey Sedykh (isolation and biochemical analysis of antibodies); RSF 21-75-10105 to Anna Timofeeva (analysis of bispecific antibodies); 0245-2021-0009 (121031300041-4) to Georgy Nevinsky (correlation and statistical analysis); and State Research and Development Program 5-21 to Natalya Gashnikova (work with the blood of HIV-infected patients). Publisher Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland.

PY - 2022/2

Y1 - 2022/2

N2 - Though hundreds of thousands of papers are currently being published on HIV/AIDS, only tens of hundreds of them are devoted to the antibodies generated during the disease. Most of these papers discuss antibodies in HIV/AIDS as a diagnostic tool, and some articles describe neutralizing antibodies as a promising treatment. In this paper, we used affinity chromatography and ELISA to isolate natural IgG from the blood of 26 HIV-infected patients. IgG preparations were separated into the subfractions containing different types of light chains, and catalytic activities of subfractions were analyzed. Here, we show for the first time that the blood of HIV patients contains ~20% of bispecific κλ-IgG, presented with all IgG subclasses. Analysis of DNA-hydrolyzing and amylolytic activity show that most IgG preparations and subfractions are catalytically active. Our results expand the possible biological functions of natural IgG in HIV infection.

AB - Though hundreds of thousands of papers are currently being published on HIV/AIDS, only tens of hundreds of them are devoted to the antibodies generated during the disease. Most of these papers discuss antibodies in HIV/AIDS as a diagnostic tool, and some articles describe neutralizing antibodies as a promising treatment. In this paper, we used affinity chromatography and ELISA to isolate natural IgG from the blood of 26 HIV-infected patients. IgG preparations were separated into the subfractions containing different types of light chains, and catalytic activities of subfractions were analyzed. Here, we show for the first time that the blood of HIV patients contains ~20% of bispecific κλ-IgG, presented with all IgG subclasses. Analysis of DNA-hydrolyzing and amylolytic activity show that most IgG preparations and subfractions are catalytically active. Our results expand the possible biological functions of natural IgG in HIV infection.

KW - Abzymes

KW - Amylolytic activity

KW - Bispecific antibodies

KW - Catalytic activity

KW - DNA hydrolysis

KW - DNAse activity

KW - ELISA

KW - HIV

KW - IgG

KW - Immunoglobulin G

UR - http://www.scopus.com/inward/record.url?scp=85125074090&partnerID=8YFLogxK

UR - https://www.mendeley.com/catalogue/38252432-b6d5-3220-bbda-95fc48600ffa/

U2 - 10.3390/life12020304

DO - 10.3390/life12020304

M3 - Article

C2 - 35207591

AN - SCOPUS:85125074090

VL - 12

JO - Life

JF - Life

SN - 2075-1729

IS - 2

M1 - 304

ER -

ID: 35549325