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The activity of phenoloxidase in haemolymph plasma is not a predictor of Lymantria dispar resistance to its baculovirus. / Kasianov, Nikita S.; Belousova, Irina A.; Pavlushin, Sergey V. et al.

In: PLoS ONE, Vol. 12, No. 8, 0183940, 30.08.2017, p. e0183940.

Research output: Contribution to journalArticlepeer-review

Harvard

Kasianov, NS, Belousova, IA, Pavlushin, SV, Dubovskiy, IM, Podgwaite, JD, Martemyanov, VV & Bakhvalov, SA 2017, 'The activity of phenoloxidase in haemolymph plasma is not a predictor of Lymantria dispar resistance to its baculovirus', PLoS ONE, vol. 12, no. 8, 0183940, pp. e0183940. https://doi.org/10.1371/journal.pone.0183940

APA

Kasianov, N. S., Belousova, I. A., Pavlushin, S. V., Dubovskiy, I. M., Podgwaite, J. D., Martemyanov, V. V., & Bakhvalov, S. A. (2017). The activity of phenoloxidase in haemolymph plasma is not a predictor of Lymantria dispar resistance to its baculovirus. PLoS ONE, 12(8), e0183940. [0183940]. https://doi.org/10.1371/journal.pone.0183940

Vancouver

Kasianov NS, Belousova IA, Pavlushin SV, Dubovskiy IM, Podgwaite JD, Martemyanov VV et al. The activity of phenoloxidase in haemolymph plasma is not a predictor of Lymantria dispar resistance to its baculovirus. PLoS ONE. 2017 Aug 30;12(8):e0183940. 0183940. doi: 10.1371/journal.pone.0183940

Author

Kasianov, Nikita S. ; Belousova, Irina A. ; Pavlushin, Sergey V. et al. / The activity of phenoloxidase in haemolymph plasma is not a predictor of Lymantria dispar resistance to its baculovirus. In: PLoS ONE. 2017 ; Vol. 12, No. 8. pp. e0183940.

BibTeX

@article{cc5305c2a6ea421ca215a85329c87ccb,
title = "The activity of phenoloxidase in haemolymph plasma is not a predictor of Lymantria dispar resistance to its baculovirus",
abstract = "Host innate immunity is one of the factors that determines the resistance of insects to their entomopathogens. In the research reported here we studied whether or not phenoloxidase (PO), a key enzyme in the melanogenesis component of humoral immunity of insects, plays a role in the protection of Lymantria dispar larvae from infection by L. dispar multiple nucleopolyhedrovirus. We studied two types of viral infection: overt and covert. The following lines of investigation were tested: i) the intravital individual estimation of baseline PO activity in haemolymph plasma followed by virus challenging; ii) the specific inhibition of PO activity in vivo by peroral treatment of infected larvae with phenylthiourea (PTU), a competitive inhibitor of PO; iii) the evaluation of PO activity in the haemolymph plasma after larval starvation. Starvation is a stress that activates the covert infection to an overt form. All of these experiments did not show a relationship between PO activity in haemolymph plasma of L. dispar larvae and larval susceptibility to baculovirus. Moreover, starvation-induced activation of covert viral infection to an overt form occurred in 70 percent of virus-carrying larvae against the background of a dramatic increase of PO activity in haemolymph plasma in the insects studied. Our conclusion is that in L. dispar larvae PO activity is not a predictor of host resistance to baculovirus.",
keywords = "Animals, Hemolymph/enzymology, Host-Pathogen Interactions, Monophenol Monooxygenase/metabolism, Moths/enzymology, Nucleopolyhedrovirus/physiology, MANDUCA-SEXTA, ARMIGERES SUBALBATUS, CYTOTOXIC MOLECULES, INSECT VIRUS, GYPSY-MOTH, LIFE-HISTORY, NUCLEAR-POLYHEDROSIS-VIRUS, VERTICAL TRANSMISSION, IMMUNE FUNCTION, DISEASE RESISTANCE",
author = "Kasianov, {Nikita S.} and Belousova, {Irina A.} and Pavlushin, {Sergey V.} and Dubovskiy, {Ivan M.} and Podgwaite, {John D.} and Martemyanov, {Vyacheslav V.} and Bakhvalov, {Stanislav A.}",
year = "2017",
month = aug,
day = "30",
doi = "10.1371/journal.pone.0183940",
language = "English",
volume = "12",
pages = "e0183940",
journal = "PLoS ONE",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "8",

}

RIS

TY - JOUR

T1 - The activity of phenoloxidase in haemolymph plasma is not a predictor of Lymantria dispar resistance to its baculovirus

AU - Kasianov, Nikita S.

AU - Belousova, Irina A.

AU - Pavlushin, Sergey V.

AU - Dubovskiy, Ivan M.

AU - Podgwaite, John D.

AU - Martemyanov, Vyacheslav V.

AU - Bakhvalov, Stanislav A.

PY - 2017/8/30

Y1 - 2017/8/30

N2 - Host innate immunity is one of the factors that determines the resistance of insects to their entomopathogens. In the research reported here we studied whether or not phenoloxidase (PO), a key enzyme in the melanogenesis component of humoral immunity of insects, plays a role in the protection of Lymantria dispar larvae from infection by L. dispar multiple nucleopolyhedrovirus. We studied two types of viral infection: overt and covert. The following lines of investigation were tested: i) the intravital individual estimation of baseline PO activity in haemolymph plasma followed by virus challenging; ii) the specific inhibition of PO activity in vivo by peroral treatment of infected larvae with phenylthiourea (PTU), a competitive inhibitor of PO; iii) the evaluation of PO activity in the haemolymph plasma after larval starvation. Starvation is a stress that activates the covert infection to an overt form. All of these experiments did not show a relationship between PO activity in haemolymph plasma of L. dispar larvae and larval susceptibility to baculovirus. Moreover, starvation-induced activation of covert viral infection to an overt form occurred in 70 percent of virus-carrying larvae against the background of a dramatic increase of PO activity in haemolymph plasma in the insects studied. Our conclusion is that in L. dispar larvae PO activity is not a predictor of host resistance to baculovirus.

AB - Host innate immunity is one of the factors that determines the resistance of insects to their entomopathogens. In the research reported here we studied whether or not phenoloxidase (PO), a key enzyme in the melanogenesis component of humoral immunity of insects, plays a role in the protection of Lymantria dispar larvae from infection by L. dispar multiple nucleopolyhedrovirus. We studied two types of viral infection: overt and covert. The following lines of investigation were tested: i) the intravital individual estimation of baseline PO activity in haemolymph plasma followed by virus challenging; ii) the specific inhibition of PO activity in vivo by peroral treatment of infected larvae with phenylthiourea (PTU), a competitive inhibitor of PO; iii) the evaluation of PO activity in the haemolymph plasma after larval starvation. Starvation is a stress that activates the covert infection to an overt form. All of these experiments did not show a relationship between PO activity in haemolymph plasma of L. dispar larvae and larval susceptibility to baculovirus. Moreover, starvation-induced activation of covert viral infection to an overt form occurred in 70 percent of virus-carrying larvae against the background of a dramatic increase of PO activity in haemolymph plasma in the insects studied. Our conclusion is that in L. dispar larvae PO activity is not a predictor of host resistance to baculovirus.

KW - Animals

KW - Hemolymph/enzymology

KW - Host-Pathogen Interactions

KW - Monophenol Monooxygenase/metabolism

KW - Moths/enzymology

KW - Nucleopolyhedrovirus/physiology

KW - MANDUCA-SEXTA

KW - ARMIGERES SUBALBATUS

KW - CYTOTOXIC MOLECULES

KW - INSECT VIRUS

KW - GYPSY-MOTH

KW - LIFE-HISTORY

KW - NUCLEAR-POLYHEDROSIS-VIRUS

KW - VERTICAL TRANSMISSION

KW - IMMUNE FUNCTION

KW - DISEASE RESISTANCE

UR - http://www.scopus.com/inward/record.url?scp=85028530390&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0183940

DO - 10.1371/journal.pone.0183940

M3 - Article

C2 - 28854240

AN - SCOPUS:85028530390

VL - 12

SP - e0183940

JO - PLoS ONE

JF - PLoS ONE

SN - 1932-6203

IS - 8

M1 - 0183940

ER -

ID: 10068430