Temperature dependence of unwinding forces between complementary oligonucleotides

Standard

Temperature dependence of unwinding forces between complementary oligonucleotides. / Dultsev, Fedor N.; Kurus, Nina N.

In: Journal of Microbiological Methods, Vol. 143, 01.12.2017, p. 94-97.

Research output: Contribution to journal › Article › peer-review

Harvard

Dultsev, FN & Kurus, NN 2017, 'Temperature dependence of unwinding forces between complementary oligonucleotides', Journal of Microbiological Methods, vol. 143, pp. 94-97. https://doi.org/10.1016/j.mimet.2017.10.011

APA

Dultsev, F. N., & Kurus, N. N. (2017). Temperature dependence of unwinding forces between complementary oligonucleotides. Journal of Microbiological Methods, 143, 94-97. https://doi.org/10.1016/j.mimet.2017.10.011

Vancouver

Dultsev FN, Kurus NN. Temperature dependence of unwinding forces between complementary oligonucleotides. Journal of Microbiological Methods. 2017 Dec 1;143:94-97. doi: 10.1016/j.mimet.2017.10.011

Author

Dultsev, Fedor N. ; Kurus, Nina N. / Temperature dependence of unwinding forces between complementary oligonucleotides. In: Journal of Microbiological Methods. 2017 ; Vol. 143. pp. 94-97.

BibTeX

@article{99c14170dcd244709d56a069c54fee98,

title = "Temperature dependence of unwinding forces between complementary oligonucleotides",

abstract = "Rupture Event Scanning (REVS) was used to study oligonucleotide unwinding under mechanical load. Oligonucleotide melting temperature was successfully estimated using this method. To estimate the enthalpy of reaction, we represented denaturation process as a unimolecular reaction. This gave us the possibility to recover the force profile from the experimental data obtained in force measurements at different scanning time (reaction time) for different temperatures.",

keywords = "Bond rupture, DNA duplex, QCM, Oligonucleotides/metabolism, Nucleic Acid Conformation/radiation effects, Base Pairing, Nucleic Acid Denaturation/radiation effects, Transition Temperature",

author = "Dultsev, {Fedor N.} and Kurus, {Nina N.}",

year = "2017",

month = dec,

day = "1",

doi = "10.1016/j.mimet.2017.10.011",

language = "English",

volume = "143",

pages = "94--97",

journal = "Journal of Microbiological Methods",

issn = "0167-7012",

publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Temperature dependence of unwinding forces between complementary oligonucleotides

AU - Dultsev, Fedor N.

AU - Kurus, Nina N.

PY - 2017/12/1

Y1 - 2017/12/1

N2 - Rupture Event Scanning (REVS) was used to study oligonucleotide unwinding under mechanical load. Oligonucleotide melting temperature was successfully estimated using this method. To estimate the enthalpy of reaction, we represented denaturation process as a unimolecular reaction. This gave us the possibility to recover the force profile from the experimental data obtained in force measurements at different scanning time (reaction time) for different temperatures.

AB - Rupture Event Scanning (REVS) was used to study oligonucleotide unwinding under mechanical load. Oligonucleotide melting temperature was successfully estimated using this method. To estimate the enthalpy of reaction, we represented denaturation process as a unimolecular reaction. This gave us the possibility to recover the force profile from the experimental data obtained in force measurements at different scanning time (reaction time) for different temperatures.

KW - Bond rupture

KW - DNA duplex

KW - QCM

KW - Oligonucleotides/metabolism

KW - Nucleic Acid Conformation/radiation effects

KW - Base Pairing

KW - Nucleic Acid Denaturation/radiation effects

KW - Transition Temperature

UR - http://www.scopus.com/inward/record.url?scp=85032353757&partnerID=8YFLogxK

U2 - 10.1016/j.mimet.2017.10.011

DO - 10.1016/j.mimet.2017.10.011

M3 - Article

C2 - 29079297

AN - SCOPUS:85032353757

VL - 143

SP - 94

EP - 97

JO - Journal of Microbiological Methods

JF - Journal of Microbiological Methods

SN - 0167-7012

ER -

ID: 9443543