Study of milk exosome nucleic acids

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Study of milk exosome nucleic acids. / Kuleshova, A. E.; Purvinsh, L. V.; Burkova, E. E. et al.

In: FEBS Open Bio, Vol. 11, 07.2021, p. 121-121.

Research output: Contribution to journal › Meeting Abstract › peer-review

Harvard

Kuleshova, AE, Purvinsh, LV, Burkova, EE, Sedykh, SE & Nevinsky, GA 2021, 'Study of milk exosome nucleic acids', FEBS Open Bio, vol. 11, pp. 121-121.

APA

Kuleshova, A. E., Purvinsh, L. V., Burkova, E. E., Sedykh, S. E., & Nevinsky, G. A. (2021). Study of milk exosome nucleic acids. FEBS Open Bio, 11, 121-121.

Vancouver

Kuleshova AE, Purvinsh LV, Burkova EE, Sedykh SE , Nevinsky GA. Study of milk exosome nucleic acids. FEBS Open Bio. 2021 Jul;11:121-121.

Author

Kuleshova, A. E. ; Purvinsh, L. V. ; Burkova, E. E. et al. / Study of milk exosome nucleic acids. In: FEBS Open Bio. 2021 ; Vol. 11. pp. 121-121.

BibTeX

@article{d26fe7bf66c04eada0428864d04e2024,

title = "Study of milk exosome nucleic acids",

abstract = "Exosomes are nanovesicles contained in various biological fluids and participating in intercellular communication. The biochemical compound of exosomes demonstrates the presence of various nucleic acids, in particular, microRNA and mRNA. In this regard, exosomes are promising candidates for developing new drug delivery vehicles in personalized medicine. Among all biological fluids containing exosomes, milk is the only available on an industrial scale; therefore, the analysis of nucleic acids in milk exosomes is an urgent task.A modified protocol was applied to isolate milk exosomes, including several stages of centrifugation, ultrafiltration, ultracentrifugation, and gel chromatography.Simultaneously, the absolute amount of 20 different types of microRNA was determined in the fat fraction of bovine, goat, and horse milk. For these microRNAs, specific patterns of relative expression were determined in each type of milk. Further studies of the miRNA composition of milk fractions will reveal specific miRNA markers for each of the studied species.",

author = "Kuleshova, {A. E.} and Purvinsh, {L. V.} and Burkova, {E. E.} and Sedykh, {S. E.} and Nevinsky, {G. A.}",

note = "This research was supported by the Russian Science Foundation grant 187410055.",

year = "2021",

month = jul,

language = "English",

volume = "11",

pages = "121--121",

journal = "FEBS Open Bio",

issn = "2211-5463",

publisher = "John Wiley & Sons Inc.",

}

RIS

TY - JOUR

T1 - Study of milk exosome nucleic acids

AU - Kuleshova, A. E.

AU - Purvinsh, L. V.

AU - Burkova, E. E.

AU - Sedykh, S. E.

AU - Nevinsky, G. A.

N1 - This research was supported by the Russian Science Foundation grant 187410055.

PY - 2021/7

Y1 - 2021/7

N2 - Exosomes are nanovesicles contained in various biological fluids and participating in intercellular communication. The biochemical compound of exosomes demonstrates the presence of various nucleic acids, in particular, microRNA and mRNA. In this regard, exosomes are promising candidates for developing new drug delivery vehicles in personalized medicine. Among all biological fluids containing exosomes, milk is the only available on an industrial scale; therefore, the analysis of nucleic acids in milk exosomes is an urgent task.A modified protocol was applied to isolate milk exosomes, including several stages of centrifugation, ultrafiltration, ultracentrifugation, and gel chromatography.Simultaneously, the absolute amount of 20 different types of microRNA was determined in the fat fraction of bovine, goat, and horse milk. For these microRNAs, specific patterns of relative expression were determined in each type of milk. Further studies of the miRNA composition of milk fractions will reveal specific miRNA markers for each of the studied species.

AB - Exosomes are nanovesicles contained in various biological fluids and participating in intercellular communication. The biochemical compound of exosomes demonstrates the presence of various nucleic acids, in particular, microRNA and mRNA. In this regard, exosomes are promising candidates for developing new drug delivery vehicles in personalized medicine. Among all biological fluids containing exosomes, milk is the only available on an industrial scale; therefore, the analysis of nucleic acids in milk exosomes is an urgent task.A modified protocol was applied to isolate milk exosomes, including several stages of centrifugation, ultrafiltration, ultracentrifugation, and gel chromatography.Simultaneously, the absolute amount of 20 different types of microRNA was determined in the fat fraction of bovine, goat, and horse milk. For these microRNAs, specific patterns of relative expression were determined in each type of milk. Further studies of the miRNA composition of milk fractions will reveal specific miRNA markers for each of the studied species.

M3 - Meeting Abstract

VL - 11

SP - 121

EP - 121

JO - FEBS Open Bio

JF - FEBS Open Bio

SN - 2211-5463

ER -

ID: 29190190