Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Research › peer-review
Study of calcium signaling dynamics in single platelets using optical activation methods. / Spiryova, Darya V.; Vorob'ev, Alexei Yu; Moskalensky, Alexander E.
Biomedical Spectroscopy, Microscopy, and Imaging. ed. / Jurgen Popp; Csilla Gergely. SPIE, 2020. 113590U (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 11359).Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Research › peer-review
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TY - GEN
T1 - Study of calcium signaling dynamics in single platelets using optical activation methods
AU - Spiryova, Darya V.
AU - Vorob'ev, Alexei Yu
AU - Moskalensky, Alexander E.
PY - 2020/4/1
Y1 - 2020/4/1
N2 - Platelets are the most important participants in both normal hemostasis and pathological thrombotic process. Platelet activation needs to be studied today because management of this process is the key to progress in the treatment of atherosclerotic cardiovascular diseases. Evaluating platelet activation at the single-cell level is a promising approach for investigating platelet functions, as well as studying the action of various receptors. Previously such single-cell studies were conducted by the immobilization of platelets on the surface, which changes the platelet signaling significantly. In this paper, we describe several activation methods to overcome this limitation, in particular, by use of photolabile "caged" analogues of activation agonists. Activation can be initiated by optical pulse with the duration of tens of milliseconds. Therefore, the technique allows one to track the very early stage of activation in freely moving single platelets. In particular, it enables the assessment of the delay between the stimulus and the calcium response in platelets. The proposed method can be used for in-depth studies of platelet physiology.
AB - Platelets are the most important participants in both normal hemostasis and pathological thrombotic process. Platelet activation needs to be studied today because management of this process is the key to progress in the treatment of atherosclerotic cardiovascular diseases. Evaluating platelet activation at the single-cell level is a promising approach for investigating platelet functions, as well as studying the action of various receptors. Previously such single-cell studies were conducted by the immobilization of platelets on the surface, which changes the platelet signaling significantly. In this paper, we describe several activation methods to overcome this limitation, in particular, by use of photolabile "caged" analogues of activation agonists. Activation can be initiated by optical pulse with the duration of tens of milliseconds. Therefore, the technique allows one to track the very early stage of activation in freely moving single platelets. In particular, it enables the assessment of the delay between the stimulus and the calcium response in platelets. The proposed method can be used for in-depth studies of platelet physiology.
KW - Activation
KW - Caged ADP
KW - Caged Epinephrine
KW - Calcium oscillations
KW - Delay time
KW - Microscope
KW - Platelet
KW - TrackMate
UR - http://www.scopus.com/inward/record.url?scp=85090380691&partnerID=8YFLogxK
U2 - 10.1117/12.2559414
DO - 10.1117/12.2559414
M3 - Conference contribution
AN - SCOPUS:85090380691
T3 - Proceedings of SPIE - The International Society for Optical Engineering
BT - Biomedical Spectroscopy, Microscopy, and Imaging
A2 - Popp, Jurgen
A2 - Gergely, Csilla
PB - SPIE
T2 - Biomedical Spectroscopy, Microscopy, and Imaging 2020
Y2 - 6 April 2020 through 10 April 2020
ER -
ID: 25290175