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Study of calcium signaling dynamics in single platelets using optical activation methods. / Spiryova, Darya V.; Vorob'ev, Alexei Yu; Moskalensky, Alexander E.

Biomedical Spectroscopy, Microscopy, and Imaging. ed. / Jurgen Popp; Csilla Gergely. SPIE, 2020. 113590U (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 11359).

Research output: Chapter in Book/Report/Conference proceedingConference contributionResearchpeer-review

Harvard

Spiryova, DV, Vorob'ev, AY & Moskalensky, AE 2020, Study of calcium signaling dynamics in single platelets using optical activation methods. in J Popp & C Gergely (eds), Biomedical Spectroscopy, Microscopy, and Imaging., 113590U, Proceedings of SPIE - The International Society for Optical Engineering, vol. 11359, SPIE, Biomedical Spectroscopy, Microscopy, and Imaging 2020, Virtual, Online, France, 06.04.2020. https://doi.org/10.1117/12.2559414

APA

Spiryova, D. V., Vorob'ev, A. Y., & Moskalensky, A. E. (2020). Study of calcium signaling dynamics in single platelets using optical activation methods. In J. Popp, & C. Gergely (Eds.), Biomedical Spectroscopy, Microscopy, and Imaging [113590U] (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 11359). SPIE. https://doi.org/10.1117/12.2559414

Vancouver

Spiryova DV, Vorob'ev AY, Moskalensky AE. Study of calcium signaling dynamics in single platelets using optical activation methods. In Popp J, Gergely C, editors, Biomedical Spectroscopy, Microscopy, and Imaging. SPIE. 2020. 113590U. (Proceedings of SPIE - The International Society for Optical Engineering). doi: 10.1117/12.2559414

Author

Spiryova, Darya V. ; Vorob'ev, Alexei Yu ; Moskalensky, Alexander E. / Study of calcium signaling dynamics in single platelets using optical activation methods. Biomedical Spectroscopy, Microscopy, and Imaging. editor / Jurgen Popp ; Csilla Gergely. SPIE, 2020. (Proceedings of SPIE - The International Society for Optical Engineering).

BibTeX

@inproceedings{b67484e991664b12b0b044588e05bdd7,
title = "Study of calcium signaling dynamics in single platelets using optical activation methods",
abstract = "Platelets are the most important participants in both normal hemostasis and pathological thrombotic process. Platelet activation needs to be studied today because management of this process is the key to progress in the treatment of atherosclerotic cardiovascular diseases. Evaluating platelet activation at the single-cell level is a promising approach for investigating platelet functions, as well as studying the action of various receptors. Previously such single-cell studies were conducted by the immobilization of platelets on the surface, which changes the platelet signaling significantly. In this paper, we describe several activation methods to overcome this limitation, in particular, by use of photolabile {"}caged{"} analogues of activation agonists. Activation can be initiated by optical pulse with the duration of tens of milliseconds. Therefore, the technique allows one to track the very early stage of activation in freely moving single platelets. In particular, it enables the assessment of the delay between the stimulus and the calcium response in platelets. The proposed method can be used for in-depth studies of platelet physiology.",
keywords = "Activation, Caged ADP, Caged Epinephrine, Calcium oscillations, Delay time, Microscope, Platelet, TrackMate",
author = "Spiryova, {Darya V.} and Vorob'ev, {Alexei Yu} and Moskalensky, {Alexander E.}",
year = "2020",
month = apr,
day = "1",
doi = "10.1117/12.2559414",
language = "English",
series = "Proceedings of SPIE - The International Society for Optical Engineering",
publisher = "SPIE",
editor = "Jurgen Popp and Csilla Gergely",
booktitle = "Biomedical Spectroscopy, Microscopy, and Imaging",
address = "United States",
note = "Biomedical Spectroscopy, Microscopy, and Imaging 2020 ; Conference date: 06-04-2020 Through 10-04-2020",

}

RIS

TY - GEN

T1 - Study of calcium signaling dynamics in single platelets using optical activation methods

AU - Spiryova, Darya V.

AU - Vorob'ev, Alexei Yu

AU - Moskalensky, Alexander E.

PY - 2020/4/1

Y1 - 2020/4/1

N2 - Platelets are the most important participants in both normal hemostasis and pathological thrombotic process. Platelet activation needs to be studied today because management of this process is the key to progress in the treatment of atherosclerotic cardiovascular diseases. Evaluating platelet activation at the single-cell level is a promising approach for investigating platelet functions, as well as studying the action of various receptors. Previously such single-cell studies were conducted by the immobilization of platelets on the surface, which changes the platelet signaling significantly. In this paper, we describe several activation methods to overcome this limitation, in particular, by use of photolabile "caged" analogues of activation agonists. Activation can be initiated by optical pulse with the duration of tens of milliseconds. Therefore, the technique allows one to track the very early stage of activation in freely moving single platelets. In particular, it enables the assessment of the delay between the stimulus and the calcium response in platelets. The proposed method can be used for in-depth studies of platelet physiology.

AB - Platelets are the most important participants in both normal hemostasis and pathological thrombotic process. Platelet activation needs to be studied today because management of this process is the key to progress in the treatment of atherosclerotic cardiovascular diseases. Evaluating platelet activation at the single-cell level is a promising approach for investigating platelet functions, as well as studying the action of various receptors. Previously such single-cell studies were conducted by the immobilization of platelets on the surface, which changes the platelet signaling significantly. In this paper, we describe several activation methods to overcome this limitation, in particular, by use of photolabile "caged" analogues of activation agonists. Activation can be initiated by optical pulse with the duration of tens of milliseconds. Therefore, the technique allows one to track the very early stage of activation in freely moving single platelets. In particular, it enables the assessment of the delay between the stimulus and the calcium response in platelets. The proposed method can be used for in-depth studies of platelet physiology.

KW - Activation

KW - Caged ADP

KW - Caged Epinephrine

KW - Calcium oscillations

KW - Delay time

KW - Microscope

KW - Platelet

KW - TrackMate

UR - http://www.scopus.com/inward/record.url?scp=85090380691&partnerID=8YFLogxK

U2 - 10.1117/12.2559414

DO - 10.1117/12.2559414

M3 - Conference contribution

AN - SCOPUS:85090380691

T3 - Proceedings of SPIE - The International Society for Optical Engineering

BT - Biomedical Spectroscopy, Microscopy, and Imaging

A2 - Popp, Jurgen

A2 - Gergely, Csilla

PB - SPIE

T2 - Biomedical Spectroscopy, Microscopy, and Imaging 2020

Y2 - 6 April 2020 through 10 April 2020

ER -

ID: 25290175