Research output: Contribution to journal › Article › peer-review
Sperm cryopreservation in the Far-Eastern wildcat (Prionailurus bengalensis euptilurus). / Amstislavsky, Sergei; Brusentsev, Eugeny; Kizilova, Elena et al.
In: Reproduction in Domestic Animals, Vol. 53, No. 5, 01.10.2018, p. 1219-1226.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Sperm cryopreservation in the Far-Eastern wildcat (Prionailurus bengalensis euptilurus)
AU - Amstislavsky, Sergei
AU - Brusentsev, Eugeny
AU - Kizilova, Elena
AU - Mokrousova, Valentina
AU - Kozhevnikova, Valeria
AU - Abramova, Tatyana
AU - Rozhkova, Irina
AU - Naidenko, Sergey
N1 - Publisher Copyright: © 2018 Blackwell Verlag GmbH
PY - 2018/10/1
Y1 - 2018/10/1
N2 - The Far-Eastern wildcat (Prionailurus bengalensis euptilurus) is a rare and poorly investigated nondomestic felid species. An attempt of freezing and cryopreserving Far-Eastern wildcat spermatozoa in CaniPlus Freeze (CPF) medium is reported. Sperm was collected by electroejaculation from five adult Far-Eastern wildcat captive-born males. Epididymal spermatozoa from five adult randomly bred domestic cat males were used as a reference. The viability of frozen-thawed spermatozoa evaluated by double staining with SYBR Green I and PI followed by the subsequent confocal laser scanning microscopy (CLSM) was 38.2% ± 3.0% for the domestic cat and 38.0% ± 10.2% for the Far-Eastern wildcat. The motility of frozen-thawed spermatozoa was 30.8% ± 9.8% for the domestic cat and 33.7% ± 15.1% for the Far-Eastern wildcat. Sperm morphology was assessed by light microscopy. The total percentage of normal spermatozoa after freezing and thawing was 51.9 ± 5.9 for the domestic cat and 55.0% ± 6.4% for the Far-Eastern wildcat. Defects of flagella were the most frequently observed abnormalities in both species (32.2% ± 4.8% and 30.8% ± 4.4% of all reported anomalies for the domestic cat and Far-Eastern wildcat, respectively). Domestic cat epididymal and Far-Eastern ejaculatory spermatozoa fertilized in vitro-matured oocytes of the domestic cat (30.0% ± 5.5% and 35.5% ± 15.0%, respectively). Taken together, these results suggest that the freezing of Far-Eastern wildcat spermatozoa with CPF medium is a suitable method for Felidae cryopreservation.
AB - The Far-Eastern wildcat (Prionailurus bengalensis euptilurus) is a rare and poorly investigated nondomestic felid species. An attempt of freezing and cryopreserving Far-Eastern wildcat spermatozoa in CaniPlus Freeze (CPF) medium is reported. Sperm was collected by electroejaculation from five adult Far-Eastern wildcat captive-born males. Epididymal spermatozoa from five adult randomly bred domestic cat males were used as a reference. The viability of frozen-thawed spermatozoa evaluated by double staining with SYBR Green I and PI followed by the subsequent confocal laser scanning microscopy (CLSM) was 38.2% ± 3.0% for the domestic cat and 38.0% ± 10.2% for the Far-Eastern wildcat. The motility of frozen-thawed spermatozoa was 30.8% ± 9.8% for the domestic cat and 33.7% ± 15.1% for the Far-Eastern wildcat. Sperm morphology was assessed by light microscopy. The total percentage of normal spermatozoa after freezing and thawing was 51.9 ± 5.9 for the domestic cat and 55.0% ± 6.4% for the Far-Eastern wildcat. Defects of flagella were the most frequently observed abnormalities in both species (32.2% ± 4.8% and 30.8% ± 4.4% of all reported anomalies for the domestic cat and Far-Eastern wildcat, respectively). Domestic cat epididymal and Far-Eastern ejaculatory spermatozoa fertilized in vitro-matured oocytes of the domestic cat (30.0% ± 5.5% and 35.5% ± 15.0%, respectively). Taken together, these results suggest that the freezing of Far-Eastern wildcat spermatozoa with CPF medium is a suitable method for Felidae cryopreservation.
KW - Cryopreservation
KW - Far-Eastern wildcat
KW - Heterologous IVF
KW - Homologous IVF
KW - Spermatozoa
KW - spermatozoa
KW - heterologous IVF
KW - homologous IVF
KW - cryopreservation
KW - EXTENDERS
KW - SPERMATOZOA
KW - SEMEN
KW - IN-VITRO FERTILIZATION
KW - DOMESTIC CAT
KW - Spermatozoa/physiology
KW - Cell Membrane
KW - Male
KW - Cryopreservation/veterinary
KW - Epididymis
KW - Ejaculation
KW - Sperm Motility/physiology
KW - Felidae
KW - Semen Preservation/veterinary
KW - Animals
KW - Fertilization in Vitro
UR - http://www.scopus.com/inward/record.url?scp=85050923722&partnerID=8YFLogxK
U2 - 10.1111/rda.13230
DO - 10.1111/rda.13230
M3 - Article
C2 - 29968363
AN - SCOPUS:85050923722
VL - 53
SP - 1219
EP - 1226
JO - Reproduction in Domestic Animals
JF - Reproduction in Domestic Animals
SN - 0936-6768
IS - 5
ER -
ID: 16113602