Research output: Contribution to journal › Review article › peer-review
Spectrally encoded microspheres for immunofluorescence analysis. / Sankova, Natalya; Shalaev, Pavel; Semeykina, Viktoriya et al.
In: Journal of Applied Polymer Science, Vol. 138, No. 8, 49890, 20.02.2021.Research output: Contribution to journal › Review article › peer-review
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TY - JOUR
T1 - Spectrally encoded microspheres for immunofluorescence analysis
AU - Sankova, Natalya
AU - Shalaev, Pavel
AU - Semeykina, Viktoriya
AU - Dolgushin, Sergey
AU - Odintsova, Elena
AU - Parkhomchuk, Ekaterina
N1 - Publisher Copyright: © 2020 Wiley Periodicals LLC Copyright: Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2021/2/20
Y1 - 2021/2/20
N2 - A modern immunofluorescence analysis based on spectrally encoded microspheres has found numerous and constantly growing applications in disease diagnosis, environmental supervision, and fundamental science. Here we present an overview of microsphere-based methods of multiplex immunofluorescence analysis and consider such important parameters of beads, that are crucial in most microsphere-based immunoassays, as size distribution, fluorescence stability, magnetic properties, and particle material. The preparation methods of the microspheres with tunable diameter, the introduction of various types of fluorochromes, and magnetic particles into the microspheres are discussed in details. This review also addresses the advantages and disadvantages of different approaches to implement technically bead-based immunofluorescence analysis.
AB - A modern immunofluorescence analysis based on spectrally encoded microspheres has found numerous and constantly growing applications in disease diagnosis, environmental supervision, and fundamental science. Here we present an overview of microsphere-based methods of multiplex immunofluorescence analysis and consider such important parameters of beads, that are crucial in most microsphere-based immunoassays, as size distribution, fluorescence stability, magnetic properties, and particle material. The preparation methods of the microspheres with tunable diameter, the introduction of various types of fluorochromes, and magnetic particles into the microspheres are discussed in details. This review also addresses the advantages and disadvantages of different approaches to implement technically bead-based immunofluorescence analysis.
KW - bioengineering
KW - biomedical applications
KW - functionalization of polymers
KW - synthesis and processing techniques
KW - MOLECULAR-WEIGHT
KW - DISPERSION POLYMERIZATION
KW - EMULSION
KW - IRON-OXIDE
KW - NANOPARTICLES
KW - SUSPENSION
KW - SEEDED POLYMERIZATION
KW - MONODISPERSE POLYSTYRENE MICROSPHERES
KW - REACTION PARAMETERS
KW - PARTICLE-SIZE
UR - http://www.scopus.com/inward/record.url?scp=85091614537&partnerID=8YFLogxK
UR - https://www.mendeley.com/catalogue/c8ec8e3d-1e45-3e29-9af7-b97ca1b42c80/
U2 - 10.1002/app.49890
DO - 10.1002/app.49890
M3 - Review article
AN - SCOPUS:85091614537
VL - 138
JO - Journal of Applied Polymer Science
JF - Journal of Applied Polymer Science
SN - 0021-8995
IS - 8
M1 - 49890
ER -
ID: 25687675