Standard

Silencing of Nicotiana benthamiana phytoendesaturase using dsRNA synthesized in vivo. / Cherenko, V. A.; Filipenko, E. A.; Golubeva, T. S.

In: IOP Conference Series: Earth and Environmental Science, Vol. 723, No. 2, 022041, 12.04.2021.

Research output: Contribution to journal › Conference article › peer-review

Harvard

Cherenko, VA, Filipenko, EA & Golubeva, TS 2021, 'Silencing of Nicotiana benthamiana phytoendesaturase using dsRNA synthesized in vivo', IOP Conference Series: Earth and Environmental Science, vol. 723, no. 2, 022041. https://doi.org/10.1088/1755-1315/723/2/022041

APA

Cherenko, V. A., Filipenko, E. A., & Golubeva, T. S. (2021). Silencing of Nicotiana benthamiana phytoendesaturase using dsRNA synthesized in vivo. IOP Conference Series: Earth and Environmental Science, 723(2), [022041]. https://doi.org/10.1088/1755-1315/723/2/022041

Vancouver

Cherenko VA, Filipenko EA, Golubeva TS. Silencing of Nicotiana benthamiana phytoendesaturase using dsRNA synthesized in vivo. IOP Conference Series: Earth and Environmental Science. 2021 Apr 12;723(2):022041. doi: 10.1088/1755-1315/723/2/022041

Author

Cherenko, V. A. ; Filipenko, E. A. ; Golubeva, T. S. / Silencing of Nicotiana benthamiana phytoendesaturase using dsRNA synthesized in vivo. In: IOP Conference Series: Earth and Environmental Science. 2021 ; Vol. 723, No. 2.

BibTeX

@article{778c00f7fa174ad0a956b2336852a4d9,
title = "Silencing of Nicotiana benthamiana phytoendesaturase using dsRNA synthesized in vivo",
abstract = "RNA interference (RNAi) using exogenous double-stranded RNA (dsRNA) has been used to silence the model gene of Nicotiana benthamiana phytoendesaturase. Here we report on an efficient technique for dsRNA synthesis using E. coli HT115 strain. This strain is deficient in RNase III, an enzyme that normally destroys most dsRNA in a bacterial cell and has been engineered to produce big quantities of dsRNA. We also used root treatment for dsRNA delivery to N. benthamiana plants. We found this method to be one of the most efficient ways to deliver dsRNA for plants. ",
author = "Cherenko, {V. A.} and Filipenko, {E. A.} and Golubeva, {T. S.}",
note = "Funding Information: This work is supported by the Russian Science Foundation under grant № 19-74-00067. Publisher Copyright: {\textcopyright} Published under licence by IOP Publishing Ltd. Copyright: Copyright 2021 Elsevier B.V., All rights reserved.; 2021 International Scientific and Practical Conference on Ensuring Sustainable Development in the Context of Agriculture, Green Energy, Ecology and Earth Science, ESDCA 2021 ; Conference date: 25-01-2021",
year = "2021",
month = apr,
day = "12",
doi = "10.1088/1755-1315/723/2/022041",
language = "English",
volume = "723",
journal = "IOP Conference Series: Earth and Environmental Science",
issn = "1755-1307",
publisher = "IOP Publishing Ltd.",
number = "2",

}

RIS

TY - JOUR

T1 - Silencing of Nicotiana benthamiana phytoendesaturase using dsRNA synthesized in vivo

AU - Cherenko, V. A.

AU - Filipenko, E. A.

AU - Golubeva, T. S.

N1 - Funding Information: This work is supported by the Russian Science Foundation under grant № 19-74-00067. Publisher Copyright: © Published under licence by IOP Publishing Ltd. Copyright: Copyright 2021 Elsevier B.V., All rights reserved.

PY - 2021/4/12

Y1 - 2021/4/12

N2 - RNA interference (RNAi) using exogenous double-stranded RNA (dsRNA) has been used to silence the model gene of Nicotiana benthamiana phytoendesaturase. Here we report on an efficient technique for dsRNA synthesis using E. coli HT115 strain. This strain is deficient in RNase III, an enzyme that normally destroys most dsRNA in a bacterial cell and has been engineered to produce big quantities of dsRNA. We also used root treatment for dsRNA delivery to N. benthamiana plants. We found this method to be one of the most efficient ways to deliver dsRNA for plants.

AB - RNA interference (RNAi) using exogenous double-stranded RNA (dsRNA) has been used to silence the model gene of Nicotiana benthamiana phytoendesaturase. Here we report on an efficient technique for dsRNA synthesis using E. coli HT115 strain. This strain is deficient in RNase III, an enzyme that normally destroys most dsRNA in a bacterial cell and has been engineered to produce big quantities of dsRNA. We also used root treatment for dsRNA delivery to N. benthamiana plants. We found this method to be one of the most efficient ways to deliver dsRNA for plants.

UR - http://www.scopus.com/inward/record.url?scp=85104855437&partnerID=8YFLogxK

U2 - 10.1088/1755-1315/723/2/022041

DO - 10.1088/1755-1315/723/2/022041

M3 - Conference article

AN - SCOPUS:85104855437

VL - 723

JO - IOP Conference Series: Earth and Environmental Science

JF - IOP Conference Series: Earth and Environmental Science

SN - 1755-1307

IS - 2

M1 - 022041

T2 - 2021 International Scientific and Practical Conference on Ensuring Sustainable Development in the Context of Agriculture, Green Energy, Ecology and Earth Science, ESDCA 2021

Y2 - 25 January 2021

ER -

ID: 28465821