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Significance of MiR-146a quantification in human blood plasma for the diagnosis of colorectal cancer. / Shirshova, A. N.; Shamovskaya, D. A.; Boyarskikh, U. A. et al.

In: Bulletin of Russian State Medical University, Vol. 6, No. 4, 01.07.2017, p. 28-33.

Research output: Contribution to journal › Article › peer-review

Harvard

Shirshova, AN, Shamovskaya, DA, Boyarskikh, UA, Apalko, SV, Leskov, LS, Sokolov, AV, Kovalenko, SA, Scherbak, SG, Pikalov, IV, Kel, AE & Filipenko, ML 2017, 'Significance of MiR-146a quantification in human blood plasma for the diagnosis of colorectal cancer', Bulletin of Russian State Medical University, vol. 6, no. 4, pp. 28-33. https://doi.org/10.24075/brsmu.2017-04-05

APA

Shirshova, A. N., Shamovskaya, D. A., Boyarskikh, U. A., Apalko, S. V., Leskov, L. S., Sokolov, A. V., Kovalenko, S. A., Scherbak, S. G., Pikalov, I. V., Kel, A. E., & Filipenko, M. L. (2017). Significance of MiR-146a quantification in human blood plasma for the diagnosis of colorectal cancer. Bulletin of Russian State Medical University, 6(4), 28-33. https://doi.org/10.24075/brsmu.2017-04-05

Vancouver

Shirshova AN, Shamovskaya DA, Boyarskikh UA, Apalko SV, Leskov LS, Sokolov AV et al. Significance of MiR-146a quantification in human blood plasma for the diagnosis of colorectal cancer. Bulletin of Russian State Medical University. 2017 Jul 1;6(4):28-33. doi: 10.24075/brsmu.2017-04-05

Author

Shirshova, A. N. ; Shamovskaya, D. A. ; Boyarskikh, U. A. et al. / Significance of MiR-146a quantification in human blood plasma for the diagnosis of colorectal cancer. In: Bulletin of Russian State Medical University. 2017 ; Vol. 6, No. 4. pp. 28-33.

BibTeX

@article{3e0a6d91aca842348fa44189b71827f9,
title = "Significance of MiR-146a quantification in human blood plasma for the diagnosis of colorectal cancer",
abstract = "Colorectal cancer (CRC) is one of the most common cancer types in the world. Timely diagnosis of CRC and adenomatous polyps aided by effective screening techniques can considerably reduce mortality from this disease. MicroRNAs constitute a new class of promising biomarkers for a range of human diseases including cancer. The following article assesses the diagnostic significance of miR-146a concentrations in the blood plasma of patients with colorectal cancer. The main group included patients with stages I to III colorectal cancer (n = 102); the control group comprised patients with chronic colitis, nonspecific ulcerative colitis and Crohn{\textquoteright}s disease (n = 58) and healthy individuals (n = 42). MicroRNA levels were quantified by reverse-transcription real-time PCR, revealing significantly higher miR-146a concentrations in the samples of patients with CRC than in the controls (p < 0.0001). The optimal diagnostic sensitivity determined by ROC analysis was 47.3 %, specificity was 91.5 %, with AUC = 0.79 ± 0.018. Our findings demonstrate that the studied approach does not have sufficient specificity, but still suggest that miR-146a can be included into screening tests based on quantification of other microRNAs with improved specificity.",
keywords = "Biomarker, Cancer research, Cel-238, Colorectal cancer, MicroRNA, MiR-146a, Polymerase chain reaction, Reverse transcription, Screening",
author = "Shirshova, {A. N.} and Shamovskaya, {D. A.} and Boyarskikh, {U. A.} and Apalko, {S. V.} and Leskov, {L. S.} and Sokolov, {A. V.} and Kovalenko, {S. A.} and Scherbak, {S. G.} and Pikalov, {I. V.} and Kel, {A. E.} and Filipenko, {M. L.}",
year = "2017",
month = jul,
day = "1",
doi = "10.24075/brsmu.2017-04-05",
language = "English",
volume = "6",
pages = "28--33",
journal = "Bulletin of Russian State Medical University",
issn = "2500-1094",
publisher = "Pirogov Russian National Research Medical University",
number = "4",

}

RIS

TY - JOUR

T1 - Significance of MiR-146a quantification in human blood plasma for the diagnosis of colorectal cancer

AU - Shirshova, A. N.

AU - Shamovskaya, D. A.

AU - Boyarskikh, U. A.

AU - Apalko, S. V.

AU - Leskov, L. S.

AU - Sokolov, A. V.

AU - Kovalenko, S. A.

AU - Scherbak, S. G.

AU - Pikalov, I. V.

AU - Kel, A. E.

AU - Filipenko, M. L.

PY - 2017/7/1

Y1 - 2017/7/1

N2 - Colorectal cancer (CRC) is one of the most common cancer types in the world. Timely diagnosis of CRC and adenomatous polyps aided by effective screening techniques can considerably reduce mortality from this disease. MicroRNAs constitute a new class of promising biomarkers for a range of human diseases including cancer. The following article assesses the diagnostic significance of miR-146a concentrations in the blood plasma of patients with colorectal cancer. The main group included patients with stages I to III colorectal cancer (n = 102); the control group comprised patients with chronic colitis, nonspecific ulcerative colitis and Crohn’s disease (n = 58) and healthy individuals (n = 42). MicroRNA levels were quantified by reverse-transcription real-time PCR, revealing significantly higher miR-146a concentrations in the samples of patients with CRC than in the controls (p < 0.0001). The optimal diagnostic sensitivity determined by ROC analysis was 47.3 %, specificity was 91.5 %, with AUC = 0.79 ± 0.018. Our findings demonstrate that the studied approach does not have sufficient specificity, but still suggest that miR-146a can be included into screening tests based on quantification of other microRNAs with improved specificity.

AB - Colorectal cancer (CRC) is one of the most common cancer types in the world. Timely diagnosis of CRC and adenomatous polyps aided by effective screening techniques can considerably reduce mortality from this disease. MicroRNAs constitute a new class of promising biomarkers for a range of human diseases including cancer. The following article assesses the diagnostic significance of miR-146a concentrations in the blood plasma of patients with colorectal cancer. The main group included patients with stages I to III colorectal cancer (n = 102); the control group comprised patients with chronic colitis, nonspecific ulcerative colitis and Crohn’s disease (n = 58) and healthy individuals (n = 42). MicroRNA levels were quantified by reverse-transcription real-time PCR, revealing significantly higher miR-146a concentrations in the samples of patients with CRC than in the controls (p < 0.0001). The optimal diagnostic sensitivity determined by ROC analysis was 47.3 %, specificity was 91.5 %, with AUC = 0.79 ± 0.018. Our findings demonstrate that the studied approach does not have sufficient specificity, but still suggest that miR-146a can be included into screening tests based on quantification of other microRNAs with improved specificity.

KW - Biomarker

KW - Cancer research

KW - Cel-238

KW - Colorectal cancer

KW - MicroRNA

KW - MiR-146a

KW - Polymerase chain reaction

KW - Reverse transcription

KW - Screening

UR - http://www.scopus.com/inward/record.url?scp=85032825105&partnerID=8YFLogxK

U2 - 10.24075/brsmu.2017-04-05

DO - 10.24075/brsmu.2017-04-05

M3 - Article

AN - SCOPUS:85032825105

VL - 6

SP - 28

EP - 33

JO - Bulletin of Russian State Medical University

JF - Bulletin of Russian State Medical University

SN - 2500-1094

IS - 4

ER -

ID: 9753888