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Selection of influenza virus resistant to the novel camphor-based antiviral camphecene results in loss of pathogenicity. / Zarubaev, Vladimir V.; Pushkina, Ekaterina A.; Borisevich, Sophia S. et al.

In: Virology, Vol. 524, 01.11.2018, p. 69-77.

Research output: Contribution to journal › Article › peer-review

Harvard

Zarubaev, VV, Pushkina, EA, Borisevich, SS, Galochkina, AV, Garshinina, AV, Shtro, AA, Egorova, AA, Sokolova, AS, Khursan, SL, Yarovaya, OI & Salakhutdinov, NF 2018, 'Selection of influenza virus resistant to the novel camphor-based antiviral camphecene results in loss of pathogenicity', Virology, vol. 524, pp. 69-77. https://doi.org/10.1016/j.virol.2018.08.011

APA

Zarubaev, V. V., Pushkina, E. A., Borisevich, S. S., Galochkina, A. V., Garshinina, A. V., Shtro, A. A., Egorova, A. A., Sokolova, A. S., Khursan, S. L., Yarovaya, O. I., & Salakhutdinov, N. F. (2018). Selection of influenza virus resistant to the novel camphor-based antiviral camphecene results in loss of pathogenicity. Virology, 524, 69-77. https://doi.org/10.1016/j.virol.2018.08.011

Vancouver

Zarubaev VV, Pushkina EA, Borisevich SS, Galochkina AV, Garshinina AV, Shtro AA et al. Selection of influenza virus resistant to the novel camphor-based antiviral camphecene results in loss of pathogenicity. Virology. 2018 Nov 1;524:69-77. doi: 10.1016/j.virol.2018.08.011

Author

Zarubaev, Vladimir V. ; Pushkina, Ekaterina A. ; Borisevich, Sophia S. et al. / Selection of influenza virus resistant to the novel camphor-based antiviral camphecene results in loss of pathogenicity. In: Virology. 2018 ; Vol. 524. pp. 69-77.

BibTeX

@article{5fe7f60622db41908fc7d540128b2882,
title = "Selection of influenza virus resistant to the novel camphor-based antiviral camphecene results in loss of pathogenicity",
abstract = "Due to the ability of influenza virus to develop drug resistance, the search for novel antivirals is an important goal of medical science and health care systems. We assessed the ability of the influenza virus to develop resistance to the hemagglutinin inhibitor camphecene and characterized laboratory-selected resistant strains. We showed by electron microscopy that camphecene decreases the number of virions fusing their envelopes with endosomal membranes. A 160-fold decrease in virus susceptibility was observed after six passages in cells. This was associated with the emergence of a V458L mutation in the HA2 subunit of HA and with a decrease in viral pathogenicity. Molecular modeling predicts that this substitution results in a more stable HA molecule compared to wild-type HA; and an altered camphecene-binding site. Therefore, despite the relatively rapid development of resistance, camphecene remains promising as a potential antiviral due to the low pathogenicity of resistant viruses that may arise.",
keywords = "Antivirals, Camphecene, Drug resistance, Hemagglutinin, Influenza virus, Pathogenicity",
author = "Zarubaev, {Vladimir V.} and Pushkina, {Ekaterina A.} and Borisevich, {Sophia S.} and Galochkina, {Anastasia V.} and Garshinina, {Angelica V.} and Shtro, {Anna A.} and Egorova, {Anna A.} and Sokolova, {Anastasia S.} and Khursan, {Sergey L.} and Yarovaya, {Olga I.} and Salakhutdinov, {Nariman F.}",
note = "Publisher Copyright: {\textcopyright} 2018 Elsevier Inc.",
year = "2018",
month = nov,
day = "1",
doi = "10.1016/j.virol.2018.08.011",
language = "English",
volume = "524",
pages = "69--77",
journal = "Virology",
issn = "0042-6822",
publisher = "Academic Press Inc.",

}

RIS

TY - JOUR

T1 - Selection of influenza virus resistant to the novel camphor-based antiviral camphecene results in loss of pathogenicity

AU - Zarubaev, Vladimir V.

AU - Pushkina, Ekaterina A.

AU - Borisevich, Sophia S.

AU - Galochkina, Anastasia V.

AU - Garshinina, Angelica V.

AU - Shtro, Anna A.

AU - Egorova, Anna A.

AU - Sokolova, Anastasia S.

AU - Khursan, Sergey L.

AU - Yarovaya, Olga I.

AU - Salakhutdinov, Nariman F.

N1 - Publisher Copyright: © 2018 Elsevier Inc.

PY - 2018/11/1

Y1 - 2018/11/1

N2 - Due to the ability of influenza virus to develop drug resistance, the search for novel antivirals is an important goal of medical science and health care systems. We assessed the ability of the influenza virus to develop resistance to the hemagglutinin inhibitor camphecene and characterized laboratory-selected resistant strains. We showed by electron microscopy that camphecene decreases the number of virions fusing their envelopes with endosomal membranes. A 160-fold decrease in virus susceptibility was observed after six passages in cells. This was associated with the emergence of a V458L mutation in the HA2 subunit of HA and with a decrease in viral pathogenicity. Molecular modeling predicts that this substitution results in a more stable HA molecule compared to wild-type HA; and an altered camphecene-binding site. Therefore, despite the relatively rapid development of resistance, camphecene remains promising as a potential antiviral due to the low pathogenicity of resistant viruses that may arise.

AB - Due to the ability of influenza virus to develop drug resistance, the search for novel antivirals is an important goal of medical science and health care systems. We assessed the ability of the influenza virus to develop resistance to the hemagglutinin inhibitor camphecene and characterized laboratory-selected resistant strains. We showed by electron microscopy that camphecene decreases the number of virions fusing their envelopes with endosomal membranes. A 160-fold decrease in virus susceptibility was observed after six passages in cells. This was associated with the emergence of a V458L mutation in the HA2 subunit of HA and with a decrease in viral pathogenicity. Molecular modeling predicts that this substitution results in a more stable HA molecule compared to wild-type HA; and an altered camphecene-binding site. Therefore, despite the relatively rapid development of resistance, camphecene remains promising as a potential antiviral due to the low pathogenicity of resistant viruses that may arise.

KW - Antivirals

KW - Camphecene

KW - Drug resistance

KW - Hemagglutinin

KW - Influenza virus

KW - Pathogenicity

UR - http://www.scopus.com/inward/record.url?scp=85052336245&partnerID=8YFLogxK

U2 - 10.1016/j.virol.2018.08.011

DO - 10.1016/j.virol.2018.08.011

M3 - Article

AN - SCOPUS:85052336245

VL - 524

SP - 69

EP - 77

JO - Virology

JF - Virology

SN - 0042-6822

ER -

ID: 16232666