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SDS-PAGE procedure : Application for characterization of new entirely uncharged nucleic acids analogs. / Pavlova, Anna S.; Dyudeeva, Evgeniya S.; Kupryushkin, Maxim S. et al.

In: Electrophoresis, Vol. 39, No. 4, 01.02.2018, p. 670-674.

Research output: Contribution to journal › Article › peer-review

Harvard

Pavlova, AS, Dyudeeva, ES, Kupryushkin, MS, Amirkhanov, NV, Pyshnyi, DV & Pyshnaya, IA 2018, 'SDS-PAGE procedure: Application for characterization of new entirely uncharged nucleic acids analogs', Electrophoresis, vol. 39, no. 4, pp. 670-674. https://doi.org/10.1002/elps.201700415

APA

Pavlova, A. S., Dyudeeva, E. S., Kupryushkin, M. S., Amirkhanov, N. V., Pyshnyi, D. V., & Pyshnaya, I. A. (2018). SDS-PAGE procedure: Application for characterization of new entirely uncharged nucleic acids analogs. Electrophoresis, 39(4), 670-674. https://doi.org/10.1002/elps.201700415

Vancouver

Pavlova AS, Dyudeeva ES, Kupryushkin MS, Amirkhanov NV, Pyshnyi DV, Pyshnaya IA. SDS-PAGE procedure: Application for characterization of new entirely uncharged nucleic acids analogs. Electrophoresis. 2018 Feb 1;39(4):670-674. doi: 10.1002/elps.201700415

Author

Pavlova, Anna S. ; Dyudeeva, Evgeniya S. ; Kupryushkin, Maxim S. et al. / SDS-PAGE procedure : Application for characterization of new entirely uncharged nucleic acids analogs. In: Electrophoresis. 2018 ; Vol. 39, No. 4. pp. 670-674.

BibTeX

@article{ec7f2afb40c447149b8e9087a19026fa,
title = "SDS-PAGE procedure: Application for characterization of new entirely uncharged nucleic acids analogs",
abstract = "SDS-PAGE is considered to be a universal method for size-based separation and analysis of proteins. In this study, we applied the principle of SDS-PAGE to the analysis of new entirely uncharged nucleic acid (NA) analogues, - phosphoryl guanidine oligonucleotides (PGOs). The procedure was also shown to be suitable for morpholino oligonucleotides (PMOs) and peptide nucleic acids (PNAs). It was demonstrated that SDS can establish hydrophobic interactions with these types of synthetic NAs, giving them a net negative charge and thus making these molecules mobile in polyacrylamide slab gels under the influence of an electric field.",
keywords = "Phosphoryl guanidine, PMO, SDS-PAGE, Uncharged nucleic acid analogs, DNA, PROTEINS, OLIGONUCLEOTIDES, DELIVERY, Oligonucleotides/analysis, Nucleic Acid Conformation, Electrophoresis, Polyacrylamide Gel/methods",
author = "Pavlova, {Anna S.} and Dyudeeva, {Evgeniya S.} and Kupryushkin, {Maxim S.} and Amirkhanov, {Nariman V.} and Pyshnyi, {Dmitrii V.} and Pyshnaya, {Inna A.}",
note = "Publisher Copyright: {\textcopyright} 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim",
year = "2018",
month = feb,
day = "1",
doi = "10.1002/elps.201700415",
language = "English",
volume = "39",
pages = "670--674",
journal = "Electrophoresis",
issn = "0173-0835",
publisher = "Wiley-VCH Verlag",
number = "4",

}

RIS

TY - JOUR

T1 - SDS-PAGE procedure

T2 - Application for characterization of new entirely uncharged nucleic acids analogs

AU - Pavlova, Anna S.

AU - Dyudeeva, Evgeniya S.

AU - Kupryushkin, Maxim S.

AU - Amirkhanov, Nariman V.

AU - Pyshnyi, Dmitrii V.

AU - Pyshnaya, Inna A.

N1 - Publisher Copyright: © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim

PY - 2018/2/1

Y1 - 2018/2/1

N2 - SDS-PAGE is considered to be a universal method for size-based separation and analysis of proteins. In this study, we applied the principle of SDS-PAGE to the analysis of new entirely uncharged nucleic acid (NA) analogues, - phosphoryl guanidine oligonucleotides (PGOs). The procedure was also shown to be suitable for morpholino oligonucleotides (PMOs) and peptide nucleic acids (PNAs). It was demonstrated that SDS can establish hydrophobic interactions with these types of synthetic NAs, giving them a net negative charge and thus making these molecules mobile in polyacrylamide slab gels under the influence of an electric field.

AB - SDS-PAGE is considered to be a universal method for size-based separation and analysis of proteins. In this study, we applied the principle of SDS-PAGE to the analysis of new entirely uncharged nucleic acid (NA) analogues, - phosphoryl guanidine oligonucleotides (PGOs). The procedure was also shown to be suitable for morpholino oligonucleotides (PMOs) and peptide nucleic acids (PNAs). It was demonstrated that SDS can establish hydrophobic interactions with these types of synthetic NAs, giving them a net negative charge and thus making these molecules mobile in polyacrylamide slab gels under the influence of an electric field.

KW - Phosphoryl guanidine

KW - PMO

KW - SDS-PAGE

KW - Uncharged nucleic acid analogs

KW - DNA

KW - PROTEINS

KW - OLIGONUCLEOTIDES

KW - DELIVERY

KW - Oligonucleotides/analysis

KW - Nucleic Acid Conformation

KW - Electrophoresis, Polyacrylamide Gel/methods

UR - http://www.scopus.com/inward/record.url?scp=85036539679&partnerID=8YFLogxK

U2 - 10.1002/elps.201700415

DO - 10.1002/elps.201700415

M3 - Article

C2 - 29112277

AN - SCOPUS:85036539679

VL - 39

SP - 670

EP - 674

JO - Electrophoresis

JF - Electrophoresis

SN - 0173-0835

IS - 4

ER -

ID: 9648962