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RGD Peptide–Albumin Conjugate for Endothelization of Electrospun Materials. / Cherepanova, A. V.; Akisheva, D.; Popova, T. V. et al.

In: Russian Journal of Bioorganic Chemistry, Vol. 45, No. 6, 01.11.2019, p. 793-802.

Research output: Contribution to journal › Article › peer-review

Harvard

Cherepanova, AV, Akisheva, D, Popova, TV, Chelobanov, BP, Chesalov, YA, Godovikova, TS, Karpenko, AA & Laktionov, PP 2019, 'RGD Peptide–Albumin Conjugate for Endothelization of Electrospun Materials', Russian Journal of Bioorganic Chemistry, vol. 45, no. 6, pp. 793-802. https://doi.org/10.1134/S1068162019060116

APA

Cherepanova, A. V., Akisheva, D., Popova, T. V., Chelobanov, B. P., Chesalov, Y. A., Godovikova, T. S., Karpenko, A. A., & Laktionov, P. P. (2019). RGD Peptide–Albumin Conjugate for Endothelization of Electrospun Materials. Russian Journal of Bioorganic Chemistry, 45(6), 793-802. https://doi.org/10.1134/S1068162019060116

Vancouver

Cherepanova AV, Akisheva D, Popova TV, Chelobanov BP, Chesalov YA, Godovikova TS et al. RGD Peptide–Albumin Conjugate for Endothelization of Electrospun Materials. Russian Journal of Bioorganic Chemistry. 2019 Nov 1;45(6):793-802. doi: 10.1134/S1068162019060116

Author

Cherepanova, A. V. ; Akisheva, D. ; Popova, T. V. et al. / RGD Peptide–Albumin Conjugate for Endothelization of Electrospun Materials. In: Russian Journal of Bioorganic Chemistry. 2019 ; Vol. 45, No. 6. pp. 793-802.

BibTeX

@article{12ba404424ce42bca5770c3eca3e124a,
title = "RGD Peptide–Albumin Conjugate for Endothelization of Electrospun Materials",
abstract = "To improve the endothelization of 3D matrices produced by electrospinning we propose adding cyclo(RGDfC) peptide conjugated to human serum albumin (HSA) (HSA-c(RGDfC)) to the electrospinning solution. HSA-c(RGDfC) has been prepared using a bifunctional linker with N-hydroxysuccinimide and maleimide groups, and the product has been confirmed by 1Н NMR spectroscopy. Electrospun matrices have been fabricated from the solutions of 3% polyurethane Tecoflex EG-80A with 10% HSA and 0.01–1.3% HSA–c(RGDfC) (relative to Tec) in 1,1,1,3,3,3-hexafluoro-2-propanol. The presence of HSA and the cyclo(RGDfC) peptide on the surface of electrospun matrices has been confirmed by FTIR spectroscopy. The structure of matrices has been examined by scanning electron microscopy. The ability of the matrices to facilitate the adhesion of primary human umbilical vein endotheliocytes (HUVECs) has been studied. The presence of HSA–c(RGDfC) conjugate in the matrices enhanced cellular adhesion in a dose dependent manner with a maximum effect at 1% HSA-c(RGDfC).",
keywords = "cell adhesion receptors, electrospinning, endothelization, human serum albumin, protein modification, RGD peptide, vascular grafts, VASCULAR GRAFTS, NANOFIBERS, SERUM-ALBUMIN, SURFACE MODIFICATION, CELL-ADHESION, IMMOBILIZATION, BIOMATERIALS",
author = "Cherepanova, {A. V.} and D. Akisheva and Popova, {T. V.} and Chelobanov, {B. P.} and Chesalov, {Yu A.} and Godovikova, {T. S.} and Karpenko, {A. A.} and Laktionov, {P. P.}",
note = "Publisher Copyright: {\textcopyright} 2019, Pleiades Publishing, Ltd.",
year = "2019",
month = nov,
day = "1",
doi = "10.1134/S1068162019060116",
language = "English",
volume = "45",
pages = "793--802",
journal = "Russian Journal of Bioorganic Chemistry",
issn = "1068-1620",
publisher = "MAIK NAUKA/INTERPERIODICA/SPRINGER",
number = "6",

}

RIS

TY - JOUR

T1 - RGD Peptide–Albumin Conjugate for Endothelization of Electrospun Materials

AU - Cherepanova, A. V.

AU - Akisheva, D.

AU - Popova, T. V.

AU - Chelobanov, B. P.

AU - Chesalov, Yu A.

AU - Godovikova, T. S.

AU - Karpenko, A. A.

AU - Laktionov, P. P.

N1 - Publisher Copyright: © 2019, Pleiades Publishing, Ltd.

PY - 2019/11/1

Y1 - 2019/11/1

N2 - To improve the endothelization of 3D matrices produced by electrospinning we propose adding cyclo(RGDfC) peptide conjugated to human serum albumin (HSA) (HSA-c(RGDfC)) to the electrospinning solution. HSA-c(RGDfC) has been prepared using a bifunctional linker with N-hydroxysuccinimide and maleimide groups, and the product has been confirmed by 1Н NMR spectroscopy. Electrospun matrices have been fabricated from the solutions of 3% polyurethane Tecoflex EG-80A with 10% HSA and 0.01–1.3% HSA–c(RGDfC) (relative to Tec) in 1,1,1,3,3,3-hexafluoro-2-propanol. The presence of HSA and the cyclo(RGDfC) peptide on the surface of electrospun matrices has been confirmed by FTIR spectroscopy. The structure of matrices has been examined by scanning electron microscopy. The ability of the matrices to facilitate the adhesion of primary human umbilical vein endotheliocytes (HUVECs) has been studied. The presence of HSA–c(RGDfC) conjugate in the matrices enhanced cellular adhesion in a dose dependent manner with a maximum effect at 1% HSA-c(RGDfC).

AB - To improve the endothelization of 3D matrices produced by electrospinning we propose adding cyclo(RGDfC) peptide conjugated to human serum albumin (HSA) (HSA-c(RGDfC)) to the electrospinning solution. HSA-c(RGDfC) has been prepared using a bifunctional linker with N-hydroxysuccinimide and maleimide groups, and the product has been confirmed by 1Н NMR spectroscopy. Electrospun matrices have been fabricated from the solutions of 3% polyurethane Tecoflex EG-80A with 10% HSA and 0.01–1.3% HSA–c(RGDfC) (relative to Tec) in 1,1,1,3,3,3-hexafluoro-2-propanol. The presence of HSA and the cyclo(RGDfC) peptide on the surface of electrospun matrices has been confirmed by FTIR spectroscopy. The structure of matrices has been examined by scanning electron microscopy. The ability of the matrices to facilitate the adhesion of primary human umbilical vein endotheliocytes (HUVECs) has been studied. The presence of HSA–c(RGDfC) conjugate in the matrices enhanced cellular adhesion in a dose dependent manner with a maximum effect at 1% HSA-c(RGDfC).

KW - cell adhesion receptors

KW - electrospinning

KW - endothelization

KW - human serum albumin

KW - protein modification

KW - RGD peptide

KW - vascular grafts

KW - VASCULAR GRAFTS

KW - NANOFIBERS

KW - SERUM-ALBUMIN

KW - SURFACE MODIFICATION

KW - CELL-ADHESION

KW - IMMOBILIZATION

KW - BIOMATERIALS

UR - http://www.scopus.com/inward/record.url?scp=85078596876&partnerID=8YFLogxK

U2 - 10.1134/S1068162019060116

DO - 10.1134/S1068162019060116

M3 - Article

AN - SCOPUS:85078596876

VL - 45

SP - 793

EP - 802

JO - Russian Journal of Bioorganic Chemistry

JF - Russian Journal of Bioorganic Chemistry

SN - 1068-1620

IS - 6

ER -

ID: 23257380