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Reporter-recruiting bifunctional aptasensor for bioluminescent analytical assays. / Davydova, Anna; Krasitskaya, Vasilisa; Vorobjev, Pavel et al.

In: RSC Advances, Vol. 10, No. 54, 01.09.2020, p. 32393-32399.

Research output: Contribution to journal › Article › peer-review

Harvard

Davydova, A, Krasitskaya, V, Vorobjev, P, Timoshenko, V, Tupikin, A, Kabilov, M, Frank, L, Venyaminova, A & Vorobyeva, M 2020, 'Reporter-recruiting bifunctional aptasensor for bioluminescent analytical assays', RSC Advances, vol. 10, no. 54, pp. 32393-32399. https://doi.org/10.1039/d0ra05117a

APA

Davydova, A., Krasitskaya, V., Vorobjev, P., Timoshenko, V., Tupikin, A., Kabilov, M., Frank, L., Venyaminova, A., & Vorobyeva, M. (2020). Reporter-recruiting bifunctional aptasensor for bioluminescent analytical assays. RSC Advances, 10(54), 32393-32399. https://doi.org/10.1039/d0ra05117a

Vancouver

Davydova A, Krasitskaya V, Vorobjev P, Timoshenko V, Tupikin A, Kabilov M et al. Reporter-recruiting bifunctional aptasensor for bioluminescent analytical assays. RSC Advances. 2020 Sept 1;10(54):32393-32399. doi: 10.1039/d0ra05117a

Author

Davydova, Anna ; Krasitskaya, Vasilisa ; Vorobjev, Pavel et al. / Reporter-recruiting bifunctional aptasensor for bioluminescent analytical assays. In: RSC Advances. 2020 ; Vol. 10, No. 54. pp. 32393-32399.

BibTeX

@article{d6e3b79810b348fb9d1d9d8d3a2ed2ea,
title = "Reporter-recruiting bifunctional aptasensor for bioluminescent analytical assays",
abstract = "We report a novel bioluminescent aptasensor, which consists of 2′-F-RNA aptamer modules joined into a bi-specific aptamer construct. One aptamer module binds the analyte, then after structural rearrangement the second module recruits non-covalently Ca2+-dependent photoprotein obelin from the solution, thus providing a bioluminescent signal. This concept allows using free protein as a reporter, which brings such advantages as no need for aptamer-protein conjugation, a possibility of thermal re-folding of aptamer component with no harm to a protein, and simpler detection protocol. We developed the new 2′-F-RNA aptamer for obelin, and proposed the strategy for engineering structure-switching bi-modular aptamer constructs which bind the analyte and the obelin in a sequential manner. With the use of hemoglobin as a model analyte, we showed the feasibility of utilizing the aptasensor in a fast and straightforward bioluminescent microplate assay. With a proper design of a secondary structure, this strategy of aptasensor engineering might be further extended to bi-specific aptamer-based bioluminescent sensors for other analytes of interest.",
keywords = "DNA APTAMER, RNA APTAMER, OBELIN, PURIFICATION, EXPRESSION, SEQUENCES, CLONING, TARGET",
author = "Anna Davydova and Vasilisa Krasitskaya and Pavel Vorobjev and Valentina Timoshenko and Alexey Tupikin and Marsel Kabilov and Ludmila Frank and Alya Venyaminova and Mariya Vorobyeva",
year = "2020",
month = sep,
day = "1",
doi = "10.1039/d0ra05117a",
language = "English",
volume = "10",
pages = "32393--32399",
journal = "RSC Advances",
issn = "2046-2069",
publisher = "ROYAL SOC CHEMISTRY",
number = "54",

}

RIS

TY - JOUR

T1 - Reporter-recruiting bifunctional aptasensor for bioluminescent analytical assays

AU - Davydova, Anna

AU - Krasitskaya, Vasilisa

AU - Vorobjev, Pavel

AU - Timoshenko, Valentina

AU - Tupikin, Alexey

AU - Kabilov, Marsel

AU - Frank, Ludmila

AU - Venyaminova, Alya

AU - Vorobyeva, Mariya

PY - 2020/9/1

Y1 - 2020/9/1

N2 - We report a novel bioluminescent aptasensor, which consists of 2′-F-RNA aptamer modules joined into a bi-specific aptamer construct. One aptamer module binds the analyte, then after structural rearrangement the second module recruits non-covalently Ca2+-dependent photoprotein obelin from the solution, thus providing a bioluminescent signal. This concept allows using free protein as a reporter, which brings such advantages as no need for aptamer-protein conjugation, a possibility of thermal re-folding of aptamer component with no harm to a protein, and simpler detection protocol. We developed the new 2′-F-RNA aptamer for obelin, and proposed the strategy for engineering structure-switching bi-modular aptamer constructs which bind the analyte and the obelin in a sequential manner. With the use of hemoglobin as a model analyte, we showed the feasibility of utilizing the aptasensor in a fast and straightforward bioluminescent microplate assay. With a proper design of a secondary structure, this strategy of aptasensor engineering might be further extended to bi-specific aptamer-based bioluminescent sensors for other analytes of interest.

AB - We report a novel bioluminescent aptasensor, which consists of 2′-F-RNA aptamer modules joined into a bi-specific aptamer construct. One aptamer module binds the analyte, then after structural rearrangement the second module recruits non-covalently Ca2+-dependent photoprotein obelin from the solution, thus providing a bioluminescent signal. This concept allows using free protein as a reporter, which brings such advantages as no need for aptamer-protein conjugation, a possibility of thermal re-folding of aptamer component with no harm to a protein, and simpler detection protocol. We developed the new 2′-F-RNA aptamer for obelin, and proposed the strategy for engineering structure-switching bi-modular aptamer constructs which bind the analyte and the obelin in a sequential manner. With the use of hemoglobin as a model analyte, we showed the feasibility of utilizing the aptasensor in a fast and straightforward bioluminescent microplate assay. With a proper design of a secondary structure, this strategy of aptasensor engineering might be further extended to bi-specific aptamer-based bioluminescent sensors for other analytes of interest.

KW - DNA APTAMER

KW - RNA APTAMER

KW - OBELIN

KW - PURIFICATION

KW - EXPRESSION

KW - SEQUENCES

KW - CLONING

KW - TARGET

UR - http://www.scopus.com/inward/record.url?scp=85090775893&partnerID=8YFLogxK

U2 - 10.1039/d0ra05117a

DO - 10.1039/d0ra05117a

M3 - Article

C2 - 35516485

AN - SCOPUS:85090775893

VL - 10

SP - 32393

EP - 32399

JO - RSC Advances

JF - RSC Advances

SN - 2046-2069

IS - 54

ER -

ID: 25303870