Research output: Contribution to journal › Article › peer-review
Raman spectroscopy for quantification of water-to-lipid ratio in phospholipid suspensions. / Adichtchev, S. V.; Surovtsev, N. V.
In: Vibrational Spectroscopy, Vol. 97, 01.07.2018, p. 102-105.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Raman spectroscopy for quantification of water-to-lipid ratio in phospholipid suspensions
AU - Adichtchev, S. V.
AU - Surovtsev, N. V.
PY - 2018/7/1
Y1 - 2018/7/1
N2 - In this paper, a way of quantification of the water-to-lipid ratio in aqueous suspensions of phospholipid vesicles by Raman spectroscopy is suggested. We measured the ratio of Raman intensities of the OH stretching band and the symmetric CH2-stretching mode for series of water suspensions of different phosphatidylcholines with varied water-to-phospholipid ratio. It is shown that there is a simple linear relation between the ratio of Raman intensities and the water-to-lipid ratio if the number of CH2 groups in the hydrocarbon chains is taken into account. The proportionality coefficient of this relation is determined for the gel and fluid phases of phospholipids.
AB - In this paper, a way of quantification of the water-to-lipid ratio in aqueous suspensions of phospholipid vesicles by Raman spectroscopy is suggested. We measured the ratio of Raman intensities of the OH stretching band and the symmetric CH2-stretching mode for series of water suspensions of different phosphatidylcholines with varied water-to-phospholipid ratio. It is shown that there is a simple linear relation between the ratio of Raman intensities and the water-to-lipid ratio if the number of CH2 groups in the hydrocarbon chains is taken into account. The proportionality coefficient of this relation is determined for the gel and fluid phases of phospholipids.
KW - Phospholipids
KW - Raman spectroscopy
KW - Vesicles
KW - Water concentration
UR - http://www.scopus.com/inward/record.url?scp=85048279028&partnerID=8YFLogxK
U2 - 10.1016/j.vibspec.2018.06.004
DO - 10.1016/j.vibspec.2018.06.004
M3 - Article
AN - SCOPUS:85048279028
VL - 97
SP - 102
EP - 105
JO - Vibrational Spectroscopy
JF - Vibrational Spectroscopy
SN - 0924-2031
ER -
ID: 13925368