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Raman Spectra of Proteins Deposited from Biofluids. / Pudova, S. S.; Zaytseva, Yu V.; Surovtsev, N. V.

In: Bulletin of the Russian Academy of Sciences: Physics, Vol. 88, No. Suppl 2, 20.02.2025, p. S229-S233.

Research output: Contribution to journal › Article › peer-review

Harvard

Pudova, SS, Zaytseva, YV & Surovtsev, NV 2025, 'Raman Spectra of Proteins Deposited from Biofluids', Bulletin of the Russian Academy of Sciences: Physics, vol. 88, no. Suppl 2, pp. S229-S233. https://doi.org/10.1134/S1062873824709334

APA

Pudova, S. S., Zaytseva, Y. V., & Surovtsev, N. V. (2025). Raman Spectra of Proteins Deposited from Biofluids. Bulletin of the Russian Academy of Sciences: Physics, 88(Suppl 2), S229-S233. https://doi.org/10.1134/S1062873824709334

Vancouver

Pudova SS, Zaytseva YV, Surovtsev NV. Raman Spectra of Proteins Deposited from Biofluids. Bulletin of the Russian Academy of Sciences: Physics. 2025 Feb 20;88(Suppl 2):S229-S233. doi: 10.1134/S1062873824709334

Author

Pudova, S. S. ; Zaytseva, Yu V. ; Surovtsev, N. V. / Raman Spectra of Proteins Deposited from Biofluids. In: Bulletin of the Russian Academy of Sciences: Physics. 2025 ; Vol. 88, No. Suppl 2. pp. S229-S233.

BibTeX

@article{0a41e4f9eeca4ded95437340b7121690,
title = "Raman Spectra of Proteins Deposited from Biofluids",
abstract = "Abstract: We developed the method for detecting and identifying low concentrations of protein in biofluid solutions using Raman spectroscopy. The method was tested on a solution of egg white in DMEM F-12 growth medium and applied to conditioned media after fibroblast cultivation. It is proposed to precipitate the protein from the solution with trichloroacetic acid to remove the contribution of non-protein components and add a reporter protein for quantitative determination of concentration.",
keywords = "Raman scattering, conditioned medium, protein precipitation, proteins, quantitative analysis",
author = "Pudova, {S. S.} and Zaytseva, {Yu V.} and Surovtsev, {N. V.}",
note = "This work was supported by Ministry of Science and Higher Education of the Russian Federation, grant no. FWNG-2024-0023.",
year = "2025",
month = feb,
day = "20",
doi = "10.1134/S1062873824709334",
language = "English",
volume = "88",
pages = "S229--S233",
journal = "Bulletin of the Russian Academy of Sciences: Physics",
issn = "1062-8738",
publisher = "Pleiades Publishing",
number = "Suppl 2",

}

RIS

TY - JOUR

T1 - Raman Spectra of Proteins Deposited from Biofluids

AU - Pudova, S. S.

AU - Zaytseva, Yu V.

AU - Surovtsev, N. V.

N1 - This work was supported by Ministry of Science and Higher Education of the Russian Federation, grant no. FWNG-2024-0023.

PY - 2025/2/20

Y1 - 2025/2/20

N2 - Abstract: We developed the method for detecting and identifying low concentrations of protein in biofluid solutions using Raman spectroscopy. The method was tested on a solution of egg white in DMEM F-12 growth medium and applied to conditioned media after fibroblast cultivation. It is proposed to precipitate the protein from the solution with trichloroacetic acid to remove the contribution of non-protein components and add a reporter protein for quantitative determination of concentration.

AB - Abstract: We developed the method for detecting and identifying low concentrations of protein in biofluid solutions using Raman spectroscopy. The method was tested on a solution of egg white in DMEM F-12 growth medium and applied to conditioned media after fibroblast cultivation. It is proposed to precipitate the protein from the solution with trichloroacetic acid to remove the contribution of non-protein components and add a reporter protein for quantitative determination of concentration.

KW - Raman scattering

KW - conditioned medium

KW - protein precipitation

KW - proteins

KW - quantitative analysis

UR - https://www.mendeley.com/catalogue/8933cc3d-4500-3775-83ac-5b49ba9395d0/

UR - https://www.scopus.com/record/display.uri?eid=2-s2.0-85218639634&origin=inward&txGid=7ca2316e1836d74ae19510c71c525382

U2 - 10.1134/S1062873824709334

DO - 10.1134/S1062873824709334

M3 - Article

VL - 88

SP - S229-S233

JO - Bulletin of the Russian Academy of Sciences: Physics

JF - Bulletin of the Russian Academy of Sciences: Physics

SN - 1062-8738

IS - Suppl 2

ER -

ID: 64917415