Quercetin Attenuates Benzo(α)pyrene-induced CYP1A Expression

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Quercetin Attenuates Benzo(α)pyrene-induced CYP1A Expression. / Perepechaeva, M. L.; Seredina, T. A.; Sidorova, Y. A. et al.

In: Biomedical and Environmental Sciences, Vol. 30, No. 4, 01.04.2017, p. 308-313.

Research output: Contribution to journal › Article › peer-review

Harvard

Perepechaeva, ML, Seredina, TA, Sidorova, YA, Pivovarova, EN, Markel, AL, Lyakhovich, VV & Grishanova, AY 2017, 'Quercetin Attenuates Benzo(α)pyrene-induced CYP1A Expression', Biomedical and Environmental Sciences, vol. 30, no. 4, pp. 308-313. https://doi.org/10.3967/bes2017.041

APA

Perepechaeva, M. L., Seredina, T. A., Sidorova, Y. A., Pivovarova, E. N., Markel, A. L., Lyakhovich, V. V., & Grishanova, A. Y. (2017). Quercetin Attenuates Benzo(α)pyrene-induced CYP1A Expression. Biomedical and Environmental Sciences, 30(4), 308-313. https://doi.org/10.3967/bes2017.041

Vancouver

Perepechaeva ML, Seredina TA, Sidorova YA, Pivovarova EN, Markel AL, Lyakhovich VV et al. Quercetin Attenuates Benzo(α)pyrene-induced CYP1A Expression. Biomedical and Environmental Sciences. 2017 Apr 1;30(4):308-313. doi: 10.3967/bes2017.041

Author

Perepechaeva, M. L. ; Seredina, T. A. ; Sidorova, Y. A. et al. / Quercetin Attenuates Benzo(α)pyrene-induced CYP1A Expression. In: Biomedical and Environmental Sciences. 2017 ; Vol. 30, No. 4. pp. 308-313.

BibTeX

@article{4eb25f1855ab425b9d62b79e7fbb8ed3,

title = "Quercetin Attenuates Benzo(α)pyrene-induced CYP1A Expression",

abstract = "We studied effects of nutrient quercetin on cytochromes' P450 1A (CYP1A) activities (measured spectrofluorimetrically using 7-ethoxy-resorufin for CYP1A1 and 7-methoxy-resorufin for CYP1A2 as substrates), on mRNA levels (measured by RT-PCR), and on DNA-binding activities (evaluated by an electrophoretic mobility shift assay) of proteins regulating CYP1A expression in untreated and benzo(α)pyrene (BaP)-treated rats. Wistar rats received quercetin, BaP, or both once daily for 1-3 days. Quercetin did not influence CYP1A1 in untreated rats but inhibited BaP-mediated CYP1A induction on the transcriptional level decreasing positive input (AhR functional activity) and increasing negative input (AhRR/ARNT expression and Oct-1 and C/EBP functional activities).",

keywords = "Animals, Antioxidants/pharmacology, Aryl Hydrocarbon Hydroxylases/genetics, Benzo(a)pyrene/toxicity, Carcinogens/toxicity, Environmental Pollutants/toxicity, Gene Expression Regulation, Male, Mutagens/toxicity, Quercetin/pharmacology, RNA, Messenger/genetics, Rats, Rats, Wistar, RAT-LIVER, PROTEIN, TRANSCRIPTION, INDUCTION, 2,3,7,8-TETRACHLORODIBENZO-P-DIOXIN, GENE, ARYL-HYDROCARBON RECEPTOR, AH-RECEPTOR, PROMOTER, BINDING",

author = "Perepechaeva, {M. L.} and Seredina, {T. A.} and Sidorova, {Y. A.} and Pivovarova, {E. N.} and Markel, {A. L.} and Lyakhovich, {V. V.} and Grishanova, {A. Y.}",

year = "2017",

month = apr,

day = "1",

doi = "10.3967/bes2017.041",

language = "English",

volume = "30",

pages = "308--313",

journal = "Biomedical and Environmental Sciences",

issn = "0895-3988",

publisher = "Elsevier",

number = "4",

}

RIS

TY - JOUR

T1 - Quercetin Attenuates Benzo(α)pyrene-induced CYP1A Expression

AU - Perepechaeva, M. L.

AU - Seredina, T. A.

AU - Sidorova, Y. A.

AU - Pivovarova, E. N.

AU - Markel, A. L.

AU - Lyakhovich, V. V.

AU - Grishanova, A. Y.

PY - 2017/4/1

Y1 - 2017/4/1

N2 - We studied effects of nutrient quercetin on cytochromes' P450 1A (CYP1A) activities (measured spectrofluorimetrically using 7-ethoxy-resorufin for CYP1A1 and 7-methoxy-resorufin for CYP1A2 as substrates), on mRNA levels (measured by RT-PCR), and on DNA-binding activities (evaluated by an electrophoretic mobility shift assay) of proteins regulating CYP1A expression in untreated and benzo(α)pyrene (BaP)-treated rats. Wistar rats received quercetin, BaP, or both once daily for 1-3 days. Quercetin did not influence CYP1A1 in untreated rats but inhibited BaP-mediated CYP1A induction on the transcriptional level decreasing positive input (AhR functional activity) and increasing negative input (AhRR/ARNT expression and Oct-1 and C/EBP functional activities).

AB - We studied effects of nutrient quercetin on cytochromes' P450 1A (CYP1A) activities (measured spectrofluorimetrically using 7-ethoxy-resorufin for CYP1A1 and 7-methoxy-resorufin for CYP1A2 as substrates), on mRNA levels (measured by RT-PCR), and on DNA-binding activities (evaluated by an electrophoretic mobility shift assay) of proteins regulating CYP1A expression in untreated and benzo(α)pyrene (BaP)-treated rats. Wistar rats received quercetin, BaP, or both once daily for 1-3 days. Quercetin did not influence CYP1A1 in untreated rats but inhibited BaP-mediated CYP1A induction on the transcriptional level decreasing positive input (AhR functional activity) and increasing negative input (AhRR/ARNT expression and Oct-1 and C/EBP functional activities).

KW - Animals

KW - Antioxidants/pharmacology

KW - Aryl Hydrocarbon Hydroxylases/genetics

KW - Benzo(a)pyrene/toxicity

KW - Carcinogens/toxicity

KW - Environmental Pollutants/toxicity

KW - Gene Expression Regulation

KW - Male

KW - Mutagens/toxicity

KW - Quercetin/pharmacology

KW - RNA, Messenger/genetics

KW - Rats

KW - Rats, Wistar

KW - RAT-LIVER

KW - PROTEIN

KW - TRANSCRIPTION

KW - INDUCTION

KW - 2,3,7,8-TETRACHLORODIBENZO-P-DIOXIN

KW - GENE

KW - ARYL-HYDROCARBON RECEPTOR

KW - AH-RECEPTOR

KW - PROMOTER

KW - BINDING

UR - http://www.scopus.com/inward/record.url?scp=85019497129&partnerID=8YFLogxK

U2 - 10.3967/bes2017.041

DO - 10.3967/bes2017.041

M3 - Article

C2 - 28494841

AN - SCOPUS:85019497129

VL - 30

SP - 308

EP - 313

JO - Biomedical and Environmental Sciences

JF - Biomedical and Environmental Sciences

SN - 0895-3988

IS - 4

ER -

ID: 10035967