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Quantitative and structural characteristics of mitochondrial DNA in varicose veins. / Smetanina, Mariya A.; Oscorbin, Igor P.; Shadrina, Alexandra S. et al.

In: Vascular Pharmacology, Vol. 145, 107021, 08.2022.

Research output: Contribution to journalArticlepeer-review

Harvard

Smetanina, MA, Oscorbin, IP, Shadrina, AS, Sevost'ianova, KS, Korolenya, VA, Gavrilov, KA, Shevela, AI, Shirshova, AN, Oskina, NA, Zolotukhin, IA & Filipenko, ML 2022, 'Quantitative and structural characteristics of mitochondrial DNA in varicose veins', Vascular Pharmacology, vol. 145, 107021. https://doi.org/10.1016/j.vph.2022.107021

APA

Smetanina, M. A., Oscorbin, I. P., Shadrina, A. S., Sevost'ianova, K. S., Korolenya, V. A., Gavrilov, K. A., Shevela, A. I., Shirshova, A. N., Oskina, N. A., Zolotukhin, I. A., & Filipenko, M. L. (2022). Quantitative and structural characteristics of mitochondrial DNA in varicose veins. Vascular Pharmacology, 145, [107021]. https://doi.org/10.1016/j.vph.2022.107021

Vancouver

Smetanina MA, Oscorbin IP, Shadrina AS, Sevost'ianova KS, Korolenya VA, Gavrilov KA et al. Quantitative and structural characteristics of mitochondrial DNA in varicose veins. Vascular Pharmacology. 2022 Aug;145:107021. Epub 2022 Jun 8. doi: 10.1016/j.vph.2022.107021

Author

Smetanina, Mariya A. ; Oscorbin, Igor P. ; Shadrina, Alexandra S. et al. / Quantitative and structural characteristics of mitochondrial DNA in varicose veins. In: Vascular Pharmacology. 2022 ; Vol. 145.

BibTeX

@article{43e4bc61232b4827b8effa60d87981c8,
title = "Quantitative and structural characteristics of mitochondrial DNA in varicose veins",
abstract = "Objective: We examined quantitative (in terms of mtDNA/nuclear DNA) and structural (in terms of common deletions in the MT-ND4 gene region) characteristics of mitochondrial DNA (mtDNA) in varicose veins (VVs) and venous wall layers by comparing mitochondrial genome parameters, as well as mitochondrial function (in terms of mitochondrial membrane potential (MtMP)), in varicose vein (VV) vs. non-varicose vein (NV) tissue samples. Methods: We analyzed paired great saphenous vein samples (VV vs. NV segments from each patient left after venous surgery) harvested from patients with VVs. Relative mtDNA level and the proportion of no-deletion mtDNA were determined by a multiplex quantitative PCR (qPCR), confirming the latter with a more sensitive method – droplet digital PCR (ddPCR). Mitochondria's functional state in VVs was assessed using fluorescent (dependent on MtMP) live-staining of mitochondria in venous tissues. Results: Total mtDNA level was lower in VV than in NV samples (predominantly in the t. media layer). ddPCR analysis showed lower proportion of no-deletion mtDNA in VVs. Because of the decrease in relative MtMP in VVs, our results suggest a possible reduction of mitochondrial function in VVs. Conclusion: Quantitative and structural changes (copy number and integrity) of mtDNA are plausibly involved in VV pathogenesis. Future clinical studies implementing the mitochondrial targeting may be eventually fostered after auxiliary mechanistic studies.",
keywords = "ddPCR, Mitochondrial DNA (mtDNA) copy number and deletions, Mitochondrial membrane potential, qPCR, Varicose veins",
author = "Smetanina, {Mariya A.} and Oscorbin, {Igor P.} and Shadrina, {Alexandra S.} and Sevost'ianova, {Kseniya S.} and Korolenya, {Valeria A.} and Gavrilov, {Konstantin A.} and Shevela, {Andrey I.} and Shirshova, {Arina N.} and Oskina, {Natalya A.} and Zolotukhin, {Igor A.} and Filipenko, {Maxim L.}",
note = "Funding Information: The work on mtDNA genotyping in the whole vein segments was supported by the Russian Science Foundation [grant number 17-75-20223 ]; the work on mtDNA genotyping and fluorescent live-staining of mitochondria in the vein wall layers, was supported by the Russian Science Foundation [grant number 22-25-00832 ]. Publisher Copyright: {\textcopyright} 2022 Elsevier Inc.",
year = "2022",
month = aug,
doi = "10.1016/j.vph.2022.107021",
language = "English",
volume = "145",
journal = "Vascular Pharmacology",
issn = "1537-1891",
publisher = "Elsevier Science Publishing Company, Inc.",

}

RIS

TY - JOUR

T1 - Quantitative and structural characteristics of mitochondrial DNA in varicose veins

AU - Smetanina, Mariya A.

AU - Oscorbin, Igor P.

AU - Shadrina, Alexandra S.

AU - Sevost'ianova, Kseniya S.

AU - Korolenya, Valeria A.

AU - Gavrilov, Konstantin A.

AU - Shevela, Andrey I.

AU - Shirshova, Arina N.

AU - Oskina, Natalya A.

AU - Zolotukhin, Igor A.

AU - Filipenko, Maxim L.

N1 - Funding Information: The work on mtDNA genotyping in the whole vein segments was supported by the Russian Science Foundation [grant number 17-75-20223 ]; the work on mtDNA genotyping and fluorescent live-staining of mitochondria in the vein wall layers, was supported by the Russian Science Foundation [grant number 22-25-00832 ]. Publisher Copyright: © 2022 Elsevier Inc.

PY - 2022/8

Y1 - 2022/8

N2 - Objective: We examined quantitative (in terms of mtDNA/nuclear DNA) and structural (in terms of common deletions in the MT-ND4 gene region) characteristics of mitochondrial DNA (mtDNA) in varicose veins (VVs) and venous wall layers by comparing mitochondrial genome parameters, as well as mitochondrial function (in terms of mitochondrial membrane potential (MtMP)), in varicose vein (VV) vs. non-varicose vein (NV) tissue samples. Methods: We analyzed paired great saphenous vein samples (VV vs. NV segments from each patient left after venous surgery) harvested from patients with VVs. Relative mtDNA level and the proportion of no-deletion mtDNA were determined by a multiplex quantitative PCR (qPCR), confirming the latter with a more sensitive method – droplet digital PCR (ddPCR). Mitochondria's functional state in VVs was assessed using fluorescent (dependent on MtMP) live-staining of mitochondria in venous tissues. Results: Total mtDNA level was lower in VV than in NV samples (predominantly in the t. media layer). ddPCR analysis showed lower proportion of no-deletion mtDNA in VVs. Because of the decrease in relative MtMP in VVs, our results suggest a possible reduction of mitochondrial function in VVs. Conclusion: Quantitative and structural changes (copy number and integrity) of mtDNA are plausibly involved in VV pathogenesis. Future clinical studies implementing the mitochondrial targeting may be eventually fostered after auxiliary mechanistic studies.

AB - Objective: We examined quantitative (in terms of mtDNA/nuclear DNA) and structural (in terms of common deletions in the MT-ND4 gene region) characteristics of mitochondrial DNA (mtDNA) in varicose veins (VVs) and venous wall layers by comparing mitochondrial genome parameters, as well as mitochondrial function (in terms of mitochondrial membrane potential (MtMP)), in varicose vein (VV) vs. non-varicose vein (NV) tissue samples. Methods: We analyzed paired great saphenous vein samples (VV vs. NV segments from each patient left after venous surgery) harvested from patients with VVs. Relative mtDNA level and the proportion of no-deletion mtDNA were determined by a multiplex quantitative PCR (qPCR), confirming the latter with a more sensitive method – droplet digital PCR (ddPCR). Mitochondria's functional state in VVs was assessed using fluorescent (dependent on MtMP) live-staining of mitochondria in venous tissues. Results: Total mtDNA level was lower in VV than in NV samples (predominantly in the t. media layer). ddPCR analysis showed lower proportion of no-deletion mtDNA in VVs. Because of the decrease in relative MtMP in VVs, our results suggest a possible reduction of mitochondrial function in VVs. Conclusion: Quantitative and structural changes (copy number and integrity) of mtDNA are plausibly involved in VV pathogenesis. Future clinical studies implementing the mitochondrial targeting may be eventually fostered after auxiliary mechanistic studies.

KW - ddPCR

KW - Mitochondrial DNA (mtDNA) copy number and deletions

KW - Mitochondrial membrane potential

KW - qPCR

KW - Varicose veins

UR - http://www.scopus.com/inward/record.url?scp=85132429981&partnerID=8YFLogxK

UR - https://www.mendeley.com/catalogue/464ec87e-fc9f-354a-a123-c69b7d9a5ba1/

U2 - 10.1016/j.vph.2022.107021

DO - 10.1016/j.vph.2022.107021

M3 - Article

C2 - 35690235

AN - SCOPUS:85132429981

VL - 145

JO - Vascular Pharmacology

JF - Vascular Pharmacology

SN - 1537-1891

M1 - 107021

ER -

ID: 36428668