Research output: Contribution to journal › Article › peer-review
QCM-based rupture force measurement as a tool to study DNA dehybridization and duplex stability. / Dultsev, Fedor N.; Kolosovsky, Eugeny A.; Lomzov, Alexander A. et al.
In: Analytical and Bioanalytical Chemistry, Vol. 409, No. 4, 02.2017, p. 891-901.Research output: Contribution to journal › Article › peer-review
}
TY - JOUR
T1 - QCM-based rupture force measurement as a tool to study DNA dehybridization and duplex stability
AU - Dultsev, Fedor N.
AU - Kolosovsky, Eugeny A.
AU - Lomzov, Alexander A.
AU - Pyshnyi, Dmitrii V.
N1 - Publisher Copyright: © 2016, Springer-Verlag Berlin Heidelberg.
PY - 2017/2
Y1 - 2017/2
N2 - The stability of double-stranded DNA (dsDNA) was assessed on the basis of unwinding force measurement. Unwinding force was measured directly with a quartz crystal microbalance (QCM). The amplitude of its surface oscillations was controlled by supplying variable alternate voltage. Under smoothly increasing amplitude of QCM surface oscillations, dsDNA fixed on QCM surface through one of its ends got unwound. This procedure allows reliable measurement of rupture force as small as 5-10 pN. It was demonstrated that oscillations of the surface, with dsDNA bound through one of its ends to this surface, at a frequency of 14 MHz, cause helix unwinding to form two complementary parts due to viscous forces of the liquid medium. Unwinding starts at the upper end. This was proven using oligonucleotide duplexes containing mismatches in different positions. For duplexes containing complementary 20 base pairs, the helix unwinding force is equal to 30-40 pN, which is in agreement with the data obtained by means of atomic-force microscopy (AFM) for the case of unzipping mode.
AB - The stability of double-stranded DNA (dsDNA) was assessed on the basis of unwinding force measurement. Unwinding force was measured directly with a quartz crystal microbalance (QCM). The amplitude of its surface oscillations was controlled by supplying variable alternate voltage. Under smoothly increasing amplitude of QCM surface oscillations, dsDNA fixed on QCM surface through one of its ends got unwound. This procedure allows reliable measurement of rupture force as small as 5-10 pN. It was demonstrated that oscillations of the surface, with dsDNA bound through one of its ends to this surface, at a frequency of 14 MHz, cause helix unwinding to form two complementary parts due to viscous forces of the liquid medium. Unwinding starts at the upper end. This was proven using oligonucleotide duplexes containing mismatches in different positions. For duplexes containing complementary 20 base pairs, the helix unwinding force is equal to 30-40 pN, which is in agreement with the data obtained by means of atomic-force microscopy (AFM) for the case of unzipping mode.
KW - Bond rupture
KW - DNA duplex
KW - QCM
KW - MECHANICAL STABILITY
KW - SINGLE
KW - SPECTROSCOPY
KW - SURFACE
KW - HYBRIDIZATION
KW - MELTING TEMPERATURES
KW - OLIGOMERS
KW - MOLECULES
KW - Viscosity
KW - Nucleic Acid Denaturation
KW - Nucleic Acid Hybridization
KW - Quartz Crystal Microbalance Techniques/methods
KW - DNA/chemistry
KW - Base Sequence
UR - http://www.scopus.com/inward/record.url?scp=84994787762&partnerID=8YFLogxK
U2 - 10.1007/s00216-016-0035-6
DO - 10.1007/s00216-016-0035-6
M3 - Article
C2 - 27838753
AN - SCOPUS:84994787762
VL - 409
SP - 891
EP - 901
JO - Analytical and Bioanalytical Chemistry
JF - Analytical and Bioanalytical Chemistry
SN - 1618-2642
IS - 4
ER -
ID: 9032475