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Program freezing of diapausing embryos in the mouse. / Amstislavsky, Sergei; Okotrub, Svetlana; Rozhkova, Irina et al.

In: Theriogenology, Vol. 217, 1, 15.03.2024, p. 1-10.

Research output: Contribution to journal › Article › peer-review

Harvard

Amstislavsky, S, Okotrub, S, Rozhkova, I, Rakhmanova, T, Igonina, T, Brusentsev, E, Kozeneva, V, Lebedeva, D, Omelchenko, A & Okotrub, K 2024, 'Program freezing of diapausing embryos in the mouse', Theriogenology, vol. 217, 1, pp. 1-10. https://doi.org/10.1016/j.theriogenology.2024.01.006

APA

Amstislavsky, S., Okotrub, S., Rozhkova, I., Rakhmanova, T., Igonina, T., Brusentsev, E., Kozeneva, V., Lebedeva, D., Omelchenko, A., & Okotrub, K. (2024). Program freezing of diapausing embryos in the mouse. Theriogenology, 217, 1-10. [1]. https://doi.org/10.1016/j.theriogenology.2024.01.006

Vancouver

Amstislavsky S, Okotrub S, Rozhkova I, Rakhmanova T, Igonina T, Brusentsev E et al. Program freezing of diapausing embryos in the mouse. Theriogenology. 2024 Mar 15;217:1-10. 1. doi: 10.1016/j.theriogenology.2024.01.006

Author

Amstislavsky, Sergei ; Okotrub, Svetlana ; Rozhkova, Irina et al. / Program freezing of diapausing embryos in the mouse. In: Theriogenology. 2024 ; Vol. 217. pp. 1-10.

BibTeX

@article{eeef7f3391914c1fb7db47ed21d41c0d,
title = "Program freezing of diapausing embryos in the mouse",
abstract = "Embryonal diapause is a characteristic feature of about 130 mammalian species. However, very few studies have addressed cryopreservation of diapausing embryos. This work is aimed to apply program freezing to blastocysts obtained from CD1 mice, which were at diapause state after ovariectomy and the subsequent hormonal therapy. Blastocysts collected from non-operated mice of the same strain served as controls. Some diapausing as well as non-diapausing frozen-thawed blastocysts demonstrated blastocoel re-expansion after 24 h of in vitro culture (IVC) indicating their viability after cryopreservation. Raman spectroscopy assessment of phenylalanine accumulation revealed that the fraction of new synthesized proteins was lower for non-frozen as well as for frozen-thawed diapausing blastocysts compared to non-diapausing ones. Although protein metabolism was reduced in diapausing embryos, most of the protein synthesis remained active. Cell number increased after 24 h of IVC in non-frozen as well as in the frozen-thawed blastocysts of the control but not of the diapause group. However, cell numbers were increased in frozen-thawed diapausing blastocysts after 47 h of IVC in a medium supplemented with putrescine. This indicates viability of frozen-thawed diapausing embryos after cryopreservation. Besides, protein metabolism was not affected by cryopreservation in diapausing and non-diapausing murine embryos indicating their viability. Our results demonstrated the possibility of successful cryopreservation of diapausing murine embryos.",
keywords = "Cryopreservation, Deuterium-labeling, Diapause, Embryos, Mice, Ovariectomy, Protein synthesis, Raman spectroscopy, Female, Mice, Animals, Freezing, Blastocyst, Cryopreservation/veterinary, Embryo, Mammalian, Mice, Inbred Strains, Mammals",
author = "Sergei Amstislavsky and Svetlana Okotrub and Irina Rozhkova and Tamara Rakhmanova and Tatyana Igonina and Eugeny Brusentsev and Varvara Kozeneva and Daria Lebedeva and Anastasia Omelchenko and Konstantin Okotrub",
note = "This study was supported by the Russian Science Foundation , project No. 23-24-00313.",
year = "2024",
month = mar,
day = "15",
doi = "10.1016/j.theriogenology.2024.01.006",
language = "English",
volume = "217",
pages = "1--10",
journal = "Theriogenology",
issn = "0093-691X",
publisher = "Elsevier Science Publishing Company, Inc.",

}

RIS

TY - JOUR

T1 - Program freezing of diapausing embryos in the mouse

AU - Amstislavsky, Sergei

AU - Okotrub, Svetlana

AU - Rozhkova, Irina

AU - Rakhmanova, Tamara

AU - Igonina, Tatyana

AU - Brusentsev, Eugeny

AU - Kozeneva, Varvara

AU - Lebedeva, Daria

AU - Omelchenko, Anastasia

AU - Okotrub, Konstantin

N1 - This study was supported by the Russian Science Foundation , project No. 23-24-00313.

PY - 2024/3/15

Y1 - 2024/3/15

N2 - Embryonal diapause is a characteristic feature of about 130 mammalian species. However, very few studies have addressed cryopreservation of diapausing embryos. This work is aimed to apply program freezing to blastocysts obtained from CD1 mice, which were at diapause state after ovariectomy and the subsequent hormonal therapy. Blastocysts collected from non-operated mice of the same strain served as controls. Some diapausing as well as non-diapausing frozen-thawed blastocysts demonstrated blastocoel re-expansion after 24 h of in vitro culture (IVC) indicating their viability after cryopreservation. Raman spectroscopy assessment of phenylalanine accumulation revealed that the fraction of new synthesized proteins was lower for non-frozen as well as for frozen-thawed diapausing blastocysts compared to non-diapausing ones. Although protein metabolism was reduced in diapausing embryos, most of the protein synthesis remained active. Cell number increased after 24 h of IVC in non-frozen as well as in the frozen-thawed blastocysts of the control but not of the diapause group. However, cell numbers were increased in frozen-thawed diapausing blastocysts after 47 h of IVC in a medium supplemented with putrescine. This indicates viability of frozen-thawed diapausing embryos after cryopreservation. Besides, protein metabolism was not affected by cryopreservation in diapausing and non-diapausing murine embryos indicating their viability. Our results demonstrated the possibility of successful cryopreservation of diapausing murine embryos.

AB - Embryonal diapause is a characteristic feature of about 130 mammalian species. However, very few studies have addressed cryopreservation of diapausing embryos. This work is aimed to apply program freezing to blastocysts obtained from CD1 mice, which were at diapause state after ovariectomy and the subsequent hormonal therapy. Blastocysts collected from non-operated mice of the same strain served as controls. Some diapausing as well as non-diapausing frozen-thawed blastocysts demonstrated blastocoel re-expansion after 24 h of in vitro culture (IVC) indicating their viability after cryopreservation. Raman spectroscopy assessment of phenylalanine accumulation revealed that the fraction of new synthesized proteins was lower for non-frozen as well as for frozen-thawed diapausing blastocysts compared to non-diapausing ones. Although protein metabolism was reduced in diapausing embryos, most of the protein synthesis remained active. Cell number increased after 24 h of IVC in non-frozen as well as in the frozen-thawed blastocysts of the control but not of the diapause group. However, cell numbers were increased in frozen-thawed diapausing blastocysts after 47 h of IVC in a medium supplemented with putrescine. This indicates viability of frozen-thawed diapausing embryos after cryopreservation. Besides, protein metabolism was not affected by cryopreservation in diapausing and non-diapausing murine embryos indicating their viability. Our results demonstrated the possibility of successful cryopreservation of diapausing murine embryos.

KW - Cryopreservation

KW - Deuterium-labeling

KW - Diapause

KW - Embryos

KW - Mice

KW - Ovariectomy

KW - Protein synthesis

KW - Raman spectroscopy

KW - Female

KW - Mice

KW - Animals

KW - Freezing

KW - Blastocyst

KW - Cryopreservation/veterinary

KW - Embryo, Mammalian

KW - Mice, Inbred Strains

KW - Mammals

UR - https://www.scopus.com/record/display.uri?eid=2-s2.0-85182379764&origin=inward&txGid=14a37eb143c2fd843ac8352eb1628d86

UR - https://www.elibrary.ru/item.asp?id=66026357

UR - https://www.mendeley.com/catalogue/f87c0cc7-d944-3e52-adba-b7847adbcd2b/

U2 - 10.1016/j.theriogenology.2024.01.006

DO - 10.1016/j.theriogenology.2024.01.006

M3 - Article

C2 - 38219408

VL - 217

SP - 1

EP - 10

JO - Theriogenology

JF - Theriogenology

SN - 0093-691X

M1 - 1

ER -

ID: 61086205