Polymorphisms of GSTM1, GSTT1, GSTP1 genes and chromosomal aberrations in lung cancer patients. / Minina, Varvara I.; Soboleva, Olga A.; Glushkov, Andrey N. et al.
In: Journal of Cancer Research and Clinical Oncology, Vol. 143, No. 11, 01.11.2017, p. 2235-2243.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Polymorphisms of GSTM1, GSTT1, GSTP1 genes and chromosomal aberrations in lung cancer patients
AU - Minina, Varvara I.
AU - Soboleva, Olga A.
AU - Glushkov, Andrey N.
AU - Voronina, Elena N.
AU - Sokolova, Ekaterina A.
AU - Bakanova, Marina L.
AU - Savchenko, Yana A.
AU - Ryzhkova, Anastasia V.
AU - Titov, Ruslan A.
AU - Druzhinin, Vladimir G.
AU - Sinitsky, Maxim Yu
AU - Asanov, Maxim A.
N1 - Publisher Copyright: © 2017, Springer-Verlag GmbH Germany.
PY - 2017/11/1
Y1 - 2017/11/1
N2 - Purpose: To study the potential links between genetic polymorphisms in the GSTT1, GSTM1, GSTP1 genes and the frequency of chromosomal aberrations (CAs) in lung cancer patients and healthy residents in Russian Federation. Methods: 200 cells in well-spread metaphase with 46 chromosomes were examined for 353 newly diagnosed lung cancer patients (males) who received medical treatment in the Kemerovo Regional Oncology Center (Kemerovo, Russian Federation), and 300 healthy males from Kemerovo, Russian Federation. The polymorphisms of the GSTM1 del and GSTT1 del genes were analysed by multiplex PCR. Genotyping of the polymorphic variants in the GSTP1 (A313G, T341C) gene was performed using Real-time PCR with competing TaqMan probes complementary to the polymorphic DNA sites. The data analysis was performed using software STATISTICA 8.0 (StatSoft Inc., USA). Results: We discovered that a GSTM1 del polymorphism increases the frequency of chromosomal damage in smoking patients with lung cancer, a general group of lung cancer patients, donors with non-small cell lung cancer and patients in the latest stages of the malignant process. The synergetic effects of occupational exposure and the malignant process can induce some modifications in the cytogenetic status in lung cancer patients harbouring the GSTM1 del polymorphism. Conclusions: CAs in peripheral blood lymphocytes can be used as biomarkers of the early biological effects of exposure to genotoxic carcinogens and may predict future cancer incidence in several epidemiologic studies. Genetic changes in genes encoding phase II detoxification enzymes are linked to decreases in the metabolic detoxification of environmentally derived genotoxic carcinogens.
AB - Purpose: To study the potential links between genetic polymorphisms in the GSTT1, GSTM1, GSTP1 genes and the frequency of chromosomal aberrations (CAs) in lung cancer patients and healthy residents in Russian Federation. Methods: 200 cells in well-spread metaphase with 46 chromosomes were examined for 353 newly diagnosed lung cancer patients (males) who received medical treatment in the Kemerovo Regional Oncology Center (Kemerovo, Russian Federation), and 300 healthy males from Kemerovo, Russian Federation. The polymorphisms of the GSTM1 del and GSTT1 del genes were analysed by multiplex PCR. Genotyping of the polymorphic variants in the GSTP1 (A313G, T341C) gene was performed using Real-time PCR with competing TaqMan probes complementary to the polymorphic DNA sites. The data analysis was performed using software STATISTICA 8.0 (StatSoft Inc., USA). Results: We discovered that a GSTM1 del polymorphism increases the frequency of chromosomal damage in smoking patients with lung cancer, a general group of lung cancer patients, donors with non-small cell lung cancer and patients in the latest stages of the malignant process. The synergetic effects of occupational exposure and the malignant process can induce some modifications in the cytogenetic status in lung cancer patients harbouring the GSTM1 del polymorphism. Conclusions: CAs in peripheral blood lymphocytes can be used as biomarkers of the early biological effects of exposure to genotoxic carcinogens and may predict future cancer incidence in several epidemiologic studies. Genetic changes in genes encoding phase II detoxification enzymes are linked to decreases in the metabolic detoxification of environmentally derived genotoxic carcinogens.
KW - Carcinogenesis
KW - Chromosomal aberrations
KW - Gene polymorphisms
KW - GST supergene family
KW - Lung cancer
KW - Xenobiotic biotransformation
KW - S-TRANSFERASE M1
KW - INDIVIDUAL SUSCEPTIBILITY
KW - CYTOGENETIC BIOMARKERS
KW - HEALTHY HUMANS
KW - RISK
KW - DAMAGE
KW - AIR-POLLUTION
KW - MOLECULAR EPIDEMIOLOGY
KW - DNA-REPAIR
KW - LYMPHOCYTES
UR - http://www.scopus.com/inward/record.url?scp=85026766650&partnerID=8YFLogxK
U2 - 10.1007/s00432-017-2486-3
DO - 10.1007/s00432-017-2486-3
M3 - Article
C2 - 28770368
AN - SCOPUS:85026766650
VL - 143
SP - 2235
EP - 2243
JO - Journal of Cancer Research and Clinical Oncology
JF - Journal of Cancer Research and Clinical Oncology
SN - 0171-5216
IS - 11
ER -
ID: 9560351