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Photoactivatable CRISPR/Cas9 System. / Akhmetova, E. A.; Golyshev, V. M.; Vokhtantcev, I. P. et al.

In: Russian Journal of Bioorganic Chemistry, Vol. 47, No. 2, 03.2021, p. 496-504.

Research output: Contribution to journal › Article › peer-review

Harvard

Akhmetova, EA, Golyshev, VM, Vokhtantcev, IP, Meschaninova, MI, Venyaminova, AG & Novopashina, DS 2021, 'Photoactivatable CRISPR/Cas9 System', Russian Journal of Bioorganic Chemistry, vol. 47, no. 2, pp. 496-504. https://doi.org/10.1134/S1068162021020023

APA

Akhmetova, E. A., Golyshev, V. M., Vokhtantcev, I. P., Meschaninova, M. I., Venyaminova, A. G., & Novopashina, D. S. (2021). Photoactivatable CRISPR/Cas9 System. Russian Journal of Bioorganic Chemistry, 47(2), 496-504. https://doi.org/10.1134/S1068162021020023

Vancouver

Akhmetova EA, Golyshev VM, Vokhtantcev IP, Meschaninova MI, Venyaminova AG, Novopashina DS. Photoactivatable CRISPR/Cas9 System. Russian Journal of Bioorganic Chemistry. 2021 Mar;47(2):496-504. doi: 10.1134/S1068162021020023

Author

Akhmetova, E. A. ; Golyshev, V. M. ; Vokhtantcev, I. P. et al. / Photoactivatable CRISPR/Cas9 System. In: Russian Journal of Bioorganic Chemistry. 2021 ; Vol. 47, No. 2. pp. 496-504.

BibTeX

@article{2b1f15bc0732485c8bba6d12d3c1537f,
title = "Photoactivatable CRISPR/Cas9 System",
abstract = "A photoactivatable CRISPR/Cas9 system consisting of the Cas9 protein, synthetic 102-nt sgRNA or a pair of guide crRNA/tracrRNA, and blocking photocleavable oligodeoxyribonucleotides has been designed. Photocleavable oligodeoxyribonucleotides contain two or three photodegradable linkers in their structure. The thermal stability of complexes of these modified oligonucleotides with guide crRNA decreases with increasing the number of linkers. The kinetics of the photomodified oligodeoxyribonucleotides cleavage has been investigated. It has been shown that UV irradiation of the CRISPR/Cas9 system in the presence of photomodified blocking oligodeoxyribonucleotides can result in photoactivatable gene editing in vitro.",
keywords = "guide RNAs for CRISPR/Cas9 system, photoactivatable gene editing in vitro, photoblocking oligodeoxyribonucleotide, photocleavable linker",
author = "Akhmetova, {E. A.} and Golyshev, {V. M.} and Vokhtantcev, {I. P.} and Meschaninova, {M. I.} and Venyaminova, {Aliya G.} and Novopashina, {D. S.}",
note = "Funding Information: The work was supported by the Russian Foundation of Basic Research (grant no. 19-34-51026). Optimization of the synthesis of extended oligodeoxyribonucleotides was supported by State-funded budget projects (Program of fundamental scientific research of the State Academies of Sciences for 2013–2020 [АААА-А17-117020210021-7]). Publisher Copyright: {\textcopyright} 2021, Pleiades Publishing, Ltd. Copyright: Copyright 2021 Elsevier B.V., All rights reserved.",
year = "2021",
month = mar,
doi = "10.1134/S1068162021020023",
language = "English",
volume = "47",
pages = "496--504",
journal = "Russian Journal of Bioorganic Chemistry",
issn = "1068-1620",
publisher = "MAIK NAUKA/INTERPERIODICA/SPRINGER",
number = "2",

}

RIS

TY - JOUR

T1 - Photoactivatable CRISPR/Cas9 System

AU - Akhmetova, E. A.

AU - Golyshev, V. M.

AU - Vokhtantcev, I. P.

AU - Meschaninova, M. I.

AU - Venyaminova, Aliya G.

AU - Novopashina, D. S.

N1 - Funding Information: The work was supported by the Russian Foundation of Basic Research (grant no. 19-34-51026). Optimization of the synthesis of extended oligodeoxyribonucleotides was supported by State-funded budget projects (Program of fundamental scientific research of the State Academies of Sciences for 2013–2020 [АААА-А17-117020210021-7]). Publisher Copyright: © 2021, Pleiades Publishing, Ltd. Copyright: Copyright 2021 Elsevier B.V., All rights reserved.

PY - 2021/3

Y1 - 2021/3

N2 - A photoactivatable CRISPR/Cas9 system consisting of the Cas9 protein, synthetic 102-nt sgRNA or a pair of guide crRNA/tracrRNA, and blocking photocleavable oligodeoxyribonucleotides has been designed. Photocleavable oligodeoxyribonucleotides contain two or three photodegradable linkers in their structure. The thermal stability of complexes of these modified oligonucleotides with guide crRNA decreases with increasing the number of linkers. The kinetics of the photomodified oligodeoxyribonucleotides cleavage has been investigated. It has been shown that UV irradiation of the CRISPR/Cas9 system in the presence of photomodified blocking oligodeoxyribonucleotides can result in photoactivatable gene editing in vitro.

AB - A photoactivatable CRISPR/Cas9 system consisting of the Cas9 protein, synthetic 102-nt sgRNA or a pair of guide crRNA/tracrRNA, and blocking photocleavable oligodeoxyribonucleotides has been designed. Photocleavable oligodeoxyribonucleotides contain two or three photodegradable linkers in their structure. The thermal stability of complexes of these modified oligonucleotides with guide crRNA decreases with increasing the number of linkers. The kinetics of the photomodified oligodeoxyribonucleotides cleavage has been investigated. It has been shown that UV irradiation of the CRISPR/Cas9 system in the presence of photomodified blocking oligodeoxyribonucleotides can result in photoactivatable gene editing in vitro.

KW - guide RNAs for CRISPR/Cas9 system

KW - photoactivatable gene editing in vitro

KW - photoblocking oligodeoxyribonucleotide

KW - photocleavable linker

UR - http://www.scopus.com/inward/record.url?scp=85104853571&partnerID=8YFLogxK

U2 - 10.1134/S1068162021020023

DO - 10.1134/S1068162021020023

M3 - Article

AN - SCOPUS:85104853571

VL - 47

SP - 496

EP - 504

JO - Russian Journal of Bioorganic Chemistry

JF - Russian Journal of Bioorganic Chemistry

SN - 1068-1620

IS - 2

ER -

ID: 28452776