TY - JOUR

T1 - Phenotypic and Genomic Analysis of Enterobacter ludwigii Strains

T2 - Insights into Mechanisms Enhancing Plant Growth Both Under Normal Conditions and in Response to Supplementation with Mineral Fertilizers and Exposure to Stress Factors

AU - Sokolova, Ekaterina Alexeevna

AU - Mishukova, Olga Viktorovna

AU - Hlistun, Inna Viktorovna

AU - Tromenschleger, Irina Nikolaevna

AU - Chumanova, Evgeniya Vladimirovna

AU - Voronina, Elena Nikolaevna

N1 - финансирование: Ministry of Education and Science of the Russian Federation 075-15-2021-1085

PY - 2024/12/19

Y1 - 2024/12/19

N2 - In this research study, we investigated four strains of Enterobacter ludwigii that showed promising properties for plant growth. These strains were tested for their ability to mobilize phosphorus and produce ammonium, siderophores, and phytohormones. The strains exhibited different values of PGP traits; however, the analysis of the complete genomes failed to reveal any significant differences in known genes associated with the expression of beneficial plant traits. One of the strains, GMG_278, demonstrated the best potential for promoting wheat growth in pot experiments. All morphological parameters of wheat were improved, both when GMG_278 was applied alone and when combined with mineral fertilizer. The combined effect we observed may suggest various mechanisms through which these treatments influence plants. The amount of pigments and proline suggests that bacterial introduction operates through pathways likely related to stress resilience. A study on the genetic mechanisms behind plant resilience to stress has revealed a significant upregulation of genes related to reactive oxygen species (ROS) defense after bacterial exposure. It is important to note that, in the initial experiments, the strain showed a significant production of salicylic acid, which is a potent inducer of oxidative stress. In addition, the synthesis of some phytohormones has been restructured, which may affect root growth and the architecture of root hairs. When combined with additional mineral fertilizers, these changes result in a significant increase in plant biomass.

AB - In this research study, we investigated four strains of Enterobacter ludwigii that showed promising properties for plant growth. These strains were tested for their ability to mobilize phosphorus and produce ammonium, siderophores, and phytohormones. The strains exhibited different values of PGP traits; however, the analysis of the complete genomes failed to reveal any significant differences in known genes associated with the expression of beneficial plant traits. One of the strains, GMG_278, demonstrated the best potential for promoting wheat growth in pot experiments. All morphological parameters of wheat were improved, both when GMG_278 was applied alone and when combined with mineral fertilizer. The combined effect we observed may suggest various mechanisms through which these treatments influence plants. The amount of pigments and proline suggests that bacterial introduction operates through pathways likely related to stress resilience. A study on the genetic mechanisms behind plant resilience to stress has revealed a significant upregulation of genes related to reactive oxygen species (ROS) defense after bacterial exposure. It is important to note that, in the initial experiments, the strain showed a significant production of salicylic acid, which is a potent inducer of oxidative stress. In addition, the synthesis of some phytohormones has been restructured, which may affect root growth and the architecture of root hairs. When combined with additional mineral fertilizers, these changes result in a significant increase in plant biomass.

KW - Enterobacter ludwigii

KW - Triticum aestivum

KW - drought

KW - mineral fertilizer

KW - plant growth-promoting bacteria

KW - reactive oxygen species

KW - salinization

UR - https://www.mendeley.com/catalogue/a9d56c4a-704b-30fd-b668-c04c0e657f32/

UR - https://www.scopus.com/record/display.uri?eid=2-s2.0-85213206091&origin=inward&txGid=6baaa206d56fb108556c849321a97005

U2 - 10.3390/plants13243551

DO - 10.3390/plants13243551

M3 - Article

C2 - 39771249

VL - 13

JO - Plants

JF - Plants

SN - 2223-7747

IS - 24

M1 - 3551

ER -