Research output: Contribution to journal › Article › peer-review
Pathogenicity assessment of wild-type and mouseadapted influenza A(H1N1) pdm09 viruses in comparison with highly pathogenic influenza A(H5N1) virus. / Prokopyeva, Elena A.; Zaykovskaya, A. V.; Sharshov, K. A. et al.
In: Histology and Histopathology, Vol. 32, No. 10, 10.2017, p. 1057-1063.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Pathogenicity assessment of wild-type and mouseadapted influenza A(H1N1) pdm09 viruses in comparison with highly pathogenic influenza A(H5N1) virus
AU - Prokopyeva, Elena A.
AU - Zaykovskaya, A. V.
AU - Sharshov, K. A.
AU - Zaykovskaya, A. V.
AU - Alekseev, A. Yu
AU - Shestopalov, A. M.
PY - 2017/10
Y1 - 2017/10
N2 - Here we compare the results of pathological and virological examinations of mice experimentally infected with either wild-type or mouse-adapted pandemic A(H1N1) pdm09 viruses and highly pathogenic avian influenza (HPAI) virus A(H5N1). Mice were sacrificed on days 1, 3, 6, and 10 post infection or whenever morbidity was severe enough to justify euthanasia. Morbidity rates were calculated on the basis of clinical signs (weight loss, poor hair coat, hunched posture and paresis); virus-induced disease was characterised by the histopathology of lung; virus dissemination was determined by virus isolation on organ samples of lung, brain, liver, kidney and spleen. All mice infected with mouse-adapted A(H1N1) pdm09 died in the course of the experiment, whereas 20% of animals survived the infection with A(H5N1). Echinocyte formation changed the rheological properties of blood in animals infected with either mouse-adapted A(H1N1) pdm09 or A(H5N1). To sum up, the adaptation of pandemic A(H1N1) pdm09 virus can confer an enhanced virulence similar to or even exceeding that of HPAI A(H5N1) virus.
AB - Here we compare the results of pathological and virological examinations of mice experimentally infected with either wild-type or mouse-adapted pandemic A(H1N1) pdm09 viruses and highly pathogenic avian influenza (HPAI) virus A(H5N1). Mice were sacrificed on days 1, 3, 6, and 10 post infection or whenever morbidity was severe enough to justify euthanasia. Morbidity rates were calculated on the basis of clinical signs (weight loss, poor hair coat, hunched posture and paresis); virus-induced disease was characterised by the histopathology of lung; virus dissemination was determined by virus isolation on organ samples of lung, brain, liver, kidney and spleen. All mice infected with mouse-adapted A(H1N1) pdm09 died in the course of the experiment, whereas 20% of animals survived the infection with A(H5N1). Echinocyte formation changed the rheological properties of blood in animals infected with either mouse-adapted A(H1N1) pdm09 or A(H5N1). To sum up, the adaptation of pandemic A(H1N1) pdm09 virus can confer an enhanced virulence similar to or even exceeding that of HPAI A(H5N1) virus.
KW - A(H1N1) pdm09
KW - A(H5N1)
KW - Echinocyte
KW - Mouseadaptation
KW - Virulence
KW - Adaptation, Biological
KW - Male
KW - Virus Shedding
KW - Influenza A Virus, H1N1 Subtype/pathogenicity
KW - Influenza A Virus, H5N1 Subtype/pathogenicity
KW - Orthomyxoviridae Infections/pathology
KW - Animals
KW - Lung/pathology
KW - Mice
KW - Mice, Inbred BALB C
KW - Mouse adaptation
KW - REPLICATION
KW - MODELS
KW - A VIRUSES
KW - ADAPTATION
KW - DISSEMINATED INTRAVASCULAR COAGULATION
KW - INFECTION
UR - http://www.scopus.com/inward/record.url?scp=85021837040&partnerID=8YFLogxK
U2 - 10.14670/HH-11-866
DO - 10.14670/HH-11-866
M3 - Article
C2 - 28083862
AN - SCOPUS:85021837040
VL - 32
SP - 1057
EP - 1063
JO - Histology and Histopathology
JF - Histology and Histopathology
SN - 0213-3911
IS - 10
ER -
ID: 10095965