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Pathogenicity assessment of wild-type and mouseadapted influenza A(H1N1) pdm09 viruses in comparison with highly pathogenic influenza A(H5N1) virus. / Prokopyeva, Elena A.; Zaykovskaya, A. V.; Sharshov, K. A. et al.

In: Histology and Histopathology, Vol. 32, No. 10, 10.2017, p. 1057-1063.

Research output: Contribution to journalArticlepeer-review

Harvard

Prokopyeva, EA, Zaykovskaya, AV, Sharshov, KA, Zaykovskaya, AV, Alekseev, AY & Shestopalov, AM 2017, 'Pathogenicity assessment of wild-type and mouseadapted influenza A(H1N1) pdm09 viruses in comparison with highly pathogenic influenza A(H5N1) virus', Histology and Histopathology, vol. 32, no. 10, pp. 1057-1063. https://doi.org/10.14670/HH-11-866

APA

Prokopyeva, E. A., Zaykovskaya, A. V., Sharshov, K. A., Zaykovskaya, A. V., Alekseev, A. Y., & Shestopalov, A. M. (2017). Pathogenicity assessment of wild-type and mouseadapted influenza A(H1N1) pdm09 viruses in comparison with highly pathogenic influenza A(H5N1) virus. Histology and Histopathology, 32(10), 1057-1063. https://doi.org/10.14670/HH-11-866

Vancouver

Prokopyeva EA, Zaykovskaya AV, Sharshov KA, Zaykovskaya AV, Alekseev AY, Shestopalov AM. Pathogenicity assessment of wild-type and mouseadapted influenza A(H1N1) pdm09 viruses in comparison with highly pathogenic influenza A(H5N1) virus. Histology and Histopathology. 2017 Oct;32(10):1057-1063. doi: 10.14670/HH-11-866

Author

Prokopyeva, Elena A. ; Zaykovskaya, A. V. ; Sharshov, K. A. et al. / Pathogenicity assessment of wild-type and mouseadapted influenza A(H1N1) pdm09 viruses in comparison with highly pathogenic influenza A(H5N1) virus. In: Histology and Histopathology. 2017 ; Vol. 32, No. 10. pp. 1057-1063.

BibTeX

@article{76b84c405c304e1183ea30001f271ab4,
title = "Pathogenicity assessment of wild-type and mouseadapted influenza A(H1N1) pdm09 viruses in comparison with highly pathogenic influenza A(H5N1) virus",
abstract = "Here we compare the results of pathological and virological examinations of mice experimentally infected with either wild-type or mouse-adapted pandemic A(H1N1) pdm09 viruses and highly pathogenic avian influenza (HPAI) virus A(H5N1). Mice were sacrificed on days 1, 3, 6, and 10 post infection or whenever morbidity was severe enough to justify euthanasia. Morbidity rates were calculated on the basis of clinical signs (weight loss, poor hair coat, hunched posture and paresis); virus-induced disease was characterised by the histopathology of lung; virus dissemination was determined by virus isolation on organ samples of lung, brain, liver, kidney and spleen. All mice infected with mouse-adapted A(H1N1) pdm09 died in the course of the experiment, whereas 20% of animals survived the infection with A(H5N1). Echinocyte formation changed the rheological properties of blood in animals infected with either mouse-adapted A(H1N1) pdm09 or A(H5N1). To sum up, the adaptation of pandemic A(H1N1) pdm09 virus can confer an enhanced virulence similar to or even exceeding that of HPAI A(H5N1) virus.",
keywords = "A(H1N1) pdm09, A(H5N1), Echinocyte, Mouseadaptation, Virulence, Adaptation, Biological, Male, Virus Shedding, Influenza A Virus, H1N1 Subtype/pathogenicity, Influenza A Virus, H5N1 Subtype/pathogenicity, Orthomyxoviridae Infections/pathology, Animals, Lung/pathology, Mice, Mice, Inbred BALB C, Mouse adaptation, REPLICATION, MODELS, A VIRUSES, ADAPTATION, DISSEMINATED INTRAVASCULAR COAGULATION, INFECTION",
author = "Prokopyeva, {Elena A.} and Zaykovskaya, {A. V.} and Sharshov, {K. A.} and Zaykovskaya, {A. V.} and Alekseev, {A. Yu} and Shestopalov, {A. M.}",
year = "2017",
month = oct,
doi = "10.14670/HH-11-866",
language = "English",
volume = "32",
pages = "1057--1063",
journal = "Histology and Histopathology",
issn = "0213-3911",
publisher = "Histology and Histopathology",
number = "10",

}

RIS

TY - JOUR

T1 - Pathogenicity assessment of wild-type and mouseadapted influenza A(H1N1) pdm09 viruses in comparison with highly pathogenic influenza A(H5N1) virus

AU - Prokopyeva, Elena A.

AU - Zaykovskaya, A. V.

AU - Sharshov, K. A.

AU - Zaykovskaya, A. V.

AU - Alekseev, A. Yu

AU - Shestopalov, A. M.

PY - 2017/10

Y1 - 2017/10

N2 - Here we compare the results of pathological and virological examinations of mice experimentally infected with either wild-type or mouse-adapted pandemic A(H1N1) pdm09 viruses and highly pathogenic avian influenza (HPAI) virus A(H5N1). Mice were sacrificed on days 1, 3, 6, and 10 post infection or whenever morbidity was severe enough to justify euthanasia. Morbidity rates were calculated on the basis of clinical signs (weight loss, poor hair coat, hunched posture and paresis); virus-induced disease was characterised by the histopathology of lung; virus dissemination was determined by virus isolation on organ samples of lung, brain, liver, kidney and spleen. All mice infected with mouse-adapted A(H1N1) pdm09 died in the course of the experiment, whereas 20% of animals survived the infection with A(H5N1). Echinocyte formation changed the rheological properties of blood in animals infected with either mouse-adapted A(H1N1) pdm09 or A(H5N1). To sum up, the adaptation of pandemic A(H1N1) pdm09 virus can confer an enhanced virulence similar to or even exceeding that of HPAI A(H5N1) virus.

AB - Here we compare the results of pathological and virological examinations of mice experimentally infected with either wild-type or mouse-adapted pandemic A(H1N1) pdm09 viruses and highly pathogenic avian influenza (HPAI) virus A(H5N1). Mice were sacrificed on days 1, 3, 6, and 10 post infection or whenever morbidity was severe enough to justify euthanasia. Morbidity rates were calculated on the basis of clinical signs (weight loss, poor hair coat, hunched posture and paresis); virus-induced disease was characterised by the histopathology of lung; virus dissemination was determined by virus isolation on organ samples of lung, brain, liver, kidney and spleen. All mice infected with mouse-adapted A(H1N1) pdm09 died in the course of the experiment, whereas 20% of animals survived the infection with A(H5N1). Echinocyte formation changed the rheological properties of blood in animals infected with either mouse-adapted A(H1N1) pdm09 or A(H5N1). To sum up, the adaptation of pandemic A(H1N1) pdm09 virus can confer an enhanced virulence similar to or even exceeding that of HPAI A(H5N1) virus.

KW - A(H1N1) pdm09

KW - A(H5N1)

KW - Echinocyte

KW - Mouseadaptation

KW - Virulence

KW - Adaptation, Biological

KW - Male

KW - Virus Shedding

KW - Influenza A Virus, H1N1 Subtype/pathogenicity

KW - Influenza A Virus, H5N1 Subtype/pathogenicity

KW - Orthomyxoviridae Infections/pathology

KW - Animals

KW - Lung/pathology

KW - Mice

KW - Mice, Inbred BALB C

KW - Mouse adaptation

KW - REPLICATION

KW - MODELS

KW - A VIRUSES

KW - ADAPTATION

KW - DISSEMINATED INTRAVASCULAR COAGULATION

KW - INFECTION

UR - http://www.scopus.com/inward/record.url?scp=85021837040&partnerID=8YFLogxK

U2 - 10.14670/HH-11-866

DO - 10.14670/HH-11-866

M3 - Article

C2 - 28083862

AN - SCOPUS:85021837040

VL - 32

SP - 1057

EP - 1063

JO - Histology and Histopathology

JF - Histology and Histopathology

SN - 0213-3911

IS - 10

ER -

ID: 10095965