Parameters of TNF receptor co-expression in allergic and autoimmune processes: Differences and diagnostic significance. / Alshevskaya, Alina; Zhukova, Julia; Lopatnikova, Julia et al.
In: Journal of immunological methods, Vol. 520, 113525, 09.2023.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Parameters of TNF receptor co-expression in allergic and autoimmune processes: Differences and diagnostic significance
AU - Alshevskaya, Alina
AU - Zhukova, Julia
AU - Lopatnikova, Julia
AU - Shkaruba, Nadezhda
AU - Chumasova, Oksana
AU - Sizikov, Alexey
AU - Demina, Daria
AU - Nepomniashchikh, Vera
AU - Gladkikh, Viktor
AU - Sennikov, Sergey
N1 - The work was performed within a state task «The study of expression indicators of cytokine receptors and their ligands in the formation of functional properties and the type of response of cell populations of various genesis in normal and pathological conditions» registration number in USAIS RDTCW 122011800353-4. Copyright © 2023. Published by Elsevier B.V.
PY - 2023/9
Y1 - 2023/9
N2 - The authors used a method quantitative estimation density of TNFR1/TNFR2 on cells by flow cytometry with calibration particles, which allowed them to estimate the absolute number of receptors on cells regardless of the type of flow cytometer. The TNF receptor expression parameters were used to determine their association with the fact of disease and to build diagnostic models. The proposed methodological approach using a combination of flow cytometry and mathematical modeling techniques represents a promising direction for testing the diagnostic and prognostic significance of the studied biomarkers. The multifactorial regression analysis constructed on the basis of this approach made it possible to refine and supplement diagnostic schemes for determining the probability of rheumatoid arthritis and bronchial asthma in patients.
AB - The authors used a method quantitative estimation density of TNFR1/TNFR2 on cells by flow cytometry with calibration particles, which allowed them to estimate the absolute number of receptors on cells regardless of the type of flow cytometer. The TNF receptor expression parameters were used to determine their association with the fact of disease and to build diagnostic models. The proposed methodological approach using a combination of flow cytometry and mathematical modeling techniques represents a promising direction for testing the diagnostic and prognostic significance of the studied biomarkers. The multifactorial regression analysis constructed on the basis of this approach made it possible to refine and supplement diagnostic schemes for determining the probability of rheumatoid arthritis and bronchial asthma in patients.
UR - https://www.scopus.com/record/display.uri?eid=2-s2.0-85166749915&origin=inward&txGid=9641d39b21f50c783b14ecdcc03ee1f2
U2 - 10.1016/j.jim.2023.113525
DO - 10.1016/j.jim.2023.113525
M3 - Article
C2 - 37467883
VL - 520
JO - Journal of immunological methods
JF - Journal of immunological methods
SN - 0022-1759
M1 - 113525
ER -
ID: 52749863