Research output: Contribution to journal › Article › peer-review
Parallel Reaction Monitoring Mode for Atenolol Quantification in Dried Plasma Spots by Liquid Chromatography Coupled with High-Resolution Mass Spectrometry. / Aksenova, Liliya V.; Koval, Vladimir V.; Chernonosov, Alexander A.
In: Processes, Vol. 10, No. 7, 1240, 07.2022.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Parallel Reaction Monitoring Mode for Atenolol Quantification in Dried Plasma Spots by Liquid Chromatography Coupled with High-Resolution Mass Spectrometry
AU - Aksenova, Liliya V.
AU - Koval, Vladimir V.
AU - Chernonosov, Alexander A.
N1 - Funding Information: The work was supported by a publicly funded project for the Institute of Chemical Biology and Fundamental Medicine SB RAS (121031300045-2). Publisher Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2022/7
Y1 - 2022/7
N2 - In this study, we reported a rapid, sensitive, robust, and validated method for atenolol quantification in dried plasma spots (DPS) by liquid chromatography with high-resolution mass spectrometry (LC-HRMS) using parallel reaction monitoring mode (PRM). Aliquots of 25 µL human plasma were placed onto Whatman 903 Cards and air-dried. Disks (3.2 mm internal diameter) were punched, and a 100 µL working internal standard solution was added to each sample and then incubated on a shaker for 15 min at 40 °C, followed by rapid centrifugation (10,000× g, 10 s). The supernatant was transferred into 300 µL vials for subsequent LC–HRMS analysis. After chromatographic separation, atenolol and the internal standard were quantified in positive-ion parallel reaction monitoring mode by detection of all target product ions at 10 ppm tolerances. The total time of the analysis was 5 min. The calibration curve was linear in the range of 5–1000 ng/mL with interday and intraday precision levels and biases of <14.4%, and recovery was 62.9–81.0%. The atenolol in DPS was stable for ≥30 days at 25 and 4 °C. This fully validated method is selective and suitable for atenolol quantitation in DPS using LC–HRMS.
AB - In this study, we reported a rapid, sensitive, robust, and validated method for atenolol quantification in dried plasma spots (DPS) by liquid chromatography with high-resolution mass spectrometry (LC-HRMS) using parallel reaction monitoring mode (PRM). Aliquots of 25 µL human plasma were placed onto Whatman 903 Cards and air-dried. Disks (3.2 mm internal diameter) were punched, and a 100 µL working internal standard solution was added to each sample and then incubated on a shaker for 15 min at 40 °C, followed by rapid centrifugation (10,000× g, 10 s). The supernatant was transferred into 300 µL vials for subsequent LC–HRMS analysis. After chromatographic separation, atenolol and the internal standard were quantified in positive-ion parallel reaction monitoring mode by detection of all target product ions at 10 ppm tolerances. The total time of the analysis was 5 min. The calibration curve was linear in the range of 5–1000 ng/mL with interday and intraday precision levels and biases of <14.4%, and recovery was 62.9–81.0%. The atenolol in DPS was stable for ≥30 days at 25 and 4 °C. This fully validated method is selective and suitable for atenolol quantitation in DPS using LC–HRMS.
KW - atenolol
KW - DBS
KW - DPS
KW - dried plasma spot
KW - LC–HRMS
KW - parallel reaction monitoring
KW - PRM
UR - http://www.scopus.com/inward/record.url?scp=85133012501&partnerID=8YFLogxK
UR - https://www.mendeley.com/catalogue/53500b0f-2bbc-36ad-92d5-970aff18604c/
U2 - 10.3390/pr10071240
DO - 10.3390/pr10071240
M3 - Article
AN - SCOPUS:85133012501
VL - 10
JO - Processes
JF - Processes
SN - 2227-9717
IS - 7
M1 - 1240
ER -
ID: 36558779