Research output: Contribution to journal › Review article › peer-review
Optogenetic and Chemical Induction Systems for Regulation of Transgene Expression in Plants: Use in Basic and Applied Research. / Omelina, Evgeniya S.; Yushkova, Anastasiya A.; Motorina, Daria M. et al.
In: International Journal of Molecular Sciences, Vol. 23, No. 3, 1737, 01.02.2022.Research output: Contribution to journal › Review article › peer-review
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TY - JOUR
T1 - Optogenetic and Chemical Induction Systems for Regulation of Transgene Expression in Plants: Use in Basic and Applied Research
AU - Omelina, Evgeniya S.
AU - Yushkova, Anastasiya A.
AU - Motorina, Daria M.
AU - Volegov, Grigorii A.
AU - Kozhevnikova, Elena N.
AU - Pindyurin, Alexey V.
N1 - Funding Information: Funding: This research was supported by a Russian Science Foundation grant (20-74-00137). Publisher Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2022/2/1
Y1 - 2022/2/1
N2 - Continuous and ubiquitous expression of foreign genes sometimes results in harmful effects on the growth, development and metabolic activities of plants. Tissue-specific promoters help to overcome this disadvantage, but do not allow one to precisely control transgene expression over time. Thus, inducible transgene expression systems have obvious benefits. In plants, transcriptional regulation is usually driven by chemical agents under the control of chemically-inducible promoters. These systems are diverse, but usually contain two elements, the chimeric transcription factor and the reporter gene. The commonly used chemically-induced expression systems are tetracycline-, steroid-, insecticide-, copper-, and ethanol-regulated. Unlike chemical-inducible systems, optogenetic tools enable spatiotemporal, quantitative and reversible control over transgene expression with light, overcoming limitations of chemically-inducible systems. This review updates and summarizes optogenetic and chemical induction methods of transgene expression used in basic plant research and discusses their potential in field applications.
AB - Continuous and ubiquitous expression of foreign genes sometimes results in harmful effects on the growth, development and metabolic activities of plants. Tissue-specific promoters help to overcome this disadvantage, but do not allow one to precisely control transgene expression over time. Thus, inducible transgene expression systems have obvious benefits. In plants, transcriptional regulation is usually driven by chemical agents under the control of chemically-inducible promoters. These systems are diverse, but usually contain two elements, the chimeric transcription factor and the reporter gene. The commonly used chemically-induced expression systems are tetracycline-, steroid-, insecticide-, copper-, and ethanol-regulated. Unlike chemical-inducible systems, optogenetic tools enable spatiotemporal, quantitative and reversible control over transgene expression with light, overcoming limitations of chemically-inducible systems. This review updates and summarizes optogenetic and chemical induction methods of transgene expression used in basic plant research and discusses their potential in field applications.
KW - Agriculture
KW - Chemical induction
KW - Optogenetics
KW - Plants
KW - Transgene expression regulation
KW - Photoreceptors, Plant/chemistry
KW - Plants/genetics
KW - Plants, Genetically Modified
KW - Gene Expression Regulation, Plant
KW - Research
KW - Transgenes
UR - http://www.scopus.com/inward/record.url?scp=85123873839&partnerID=8YFLogxK
UR - https://www.mendeley.com/catalogue/77589944-0ab8-3208-962d-b53d7e96e971/
U2 - 10.3390/ijms23031737
DO - 10.3390/ijms23031737
M3 - Review article
C2 - 35163658
AN - SCOPUS:85123873839
VL - 23
JO - International Journal of Molecular Sciences
JF - International Journal of Molecular Sciences
SN - 1661-6596
IS - 3
M1 - 1737
ER -
ID: 35429824