TY - JOUR

T1 - Oncolytic effect of wild-type Newcastle disease virus isolates in cancer cell lines in vitro and in vivo on xenograft model

AU - Yurchenko, Kseniya S.

AU - Zhou, Peipei

AU - Kovner, Anna V.

AU - Zavjalov, Evgenii L.

AU - Shestopalova, Lidiya V.

AU - Shestopalov, Alexander M.

PY - 2018/4/5

Y1 - 2018/4/5

N2 - Oncolyic virotherapy is one of the modern experimental techniques to treat human cancers. Here we studied the antitumor activity of wild-type Newcastle disease virus (NDV) isolates from Russian migratory birds. We showed that NDV could selectively kill malignant cells without affecting healthy cells. We evaluated the oncolytic effect of 44 NDV isolates in 4 his-togenetically different human cell lines (HCT116, HeLa, A549, MCF7). The safety of the isolates was also tested in normal peripheral blood mononuclear (PBMC) cells. The viability of tumor cell lines after incubation with NDV isolates was evaluated by MTT. All cell lines, except for normal PBMC primary cells, had different degrees of susceptibility to NDV infection. Seven NDV strains had the highest oncolytic activity, and some NDV strains demonstrated oncolytic selectivity for different cell lines. In vivo, we described the intratumoral activity of NDV/Altai/pigeon/770/2011 against subcutaneous non-small cell lung carcinoma using xenograft SCID mice model. All animals were responsive to therapy. Histology confirmed therapy-induced destructive changes and growing necrotic bulk density in tumor tissue. Our findings indicate that wild-type NDV strains selectively kill tumor cells with no effect on healthy PBMC cells, and intratumoral virotherapy with NDV suppresses the subcutaneous tumor growth in SCID mice.

AB - Oncolyic virotherapy is one of the modern experimental techniques to treat human cancers. Here we studied the antitumor activity of wild-type Newcastle disease virus (NDV) isolates from Russian migratory birds. We showed that NDV could selectively kill malignant cells without affecting healthy cells. We evaluated the oncolytic effect of 44 NDV isolates in 4 his-togenetically different human cell lines (HCT116, HeLa, A549, MCF7). The safety of the isolates was also tested in normal peripheral blood mononuclear (PBMC) cells. The viability of tumor cell lines after incubation with NDV isolates was evaluated by MTT. All cell lines, except for normal PBMC primary cells, had different degrees of susceptibility to NDV infection. Seven NDV strains had the highest oncolytic activity, and some NDV strains demonstrated oncolytic selectivity for different cell lines. In vivo, we described the intratumoral activity of NDV/Altai/pigeon/770/2011 against subcutaneous non-small cell lung carcinoma using xenograft SCID mice model. All animals were responsive to therapy. Histology confirmed therapy-induced destructive changes and growing necrotic bulk density in tumor tissue. Our findings indicate that wild-type NDV strains selectively kill tumor cells with no effect on healthy PBMC cells, and intratumoral virotherapy with NDV suppresses the subcutaneous tumor growth in SCID mice.

KW - A549 Cells

KW - Animals

KW - Bird Diseases/virology

KW - Birds

KW - Carcinoma, Non-Small-Cell Lung/therapy

KW - Cell Line, Tumor

KW - HCT116 Cells

KW - HeLa Cells

KW - Humans

KW - Lung Neoplasms/therapy

KW - MCF-7 Cells

KW - Mice

KW - Mice, SCID

KW - Neoplasm Transplantation

KW - Newcastle disease virus/growth & development

KW - Oncolytic Virotherapy/methods

KW - Russia

KW - Siberia

KW - Transplantation, Heterologous

KW - APOPTOSIS

KW - DEATH

KW - PANCREATIC ADENOCARCINOMA

KW - VIROTHERAPY

KW - REPLICATION

KW - INFECTION

KW - GLIOMA

KW - TRIAL

KW - MTH-68/H

KW - RESPONSES

UR - http://www.scopus.com/inward/record.url?scp=85045037225&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0195425

DO - 10.1371/journal.pone.0195425

M3 - Article

C2 - 29621357

AN - SCOPUS:85045037225

VL - 13

JO - PLoS ONE

JF - PLoS ONE

SN - 1932-6203

IS - 4

M1 - e0195425

ER -