Nuclear delivery of oligonucleotides via nanocomposites based on TiO2 nanoparticles and polylysine

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Nuclear delivery of oligonucleotides via nanocomposites based on TiO2 nanoparticles and polylysine. / Chelobanov, B. P.; Repkova, M. N.; Baiborodin, S. I. et al.

In: Molekuliarnaia biologiia, Vol. 51, No. 5, 09.11.2017, p. 797-808.

Research output: Contribution to journal › Article › peer-review

Author

Chelobanov, B. P. ; Repkova, M. N. ; Baiborodin, S. I. et al. / Nuclear delivery of oligonucleotides via nanocomposites based on TiO2 nanoparticles and polylysine. In: Molekuliarnaia biologiia. 2017 ; Vol. 51, No. 5. pp. 797-808.

BibTeX

@article{2358c934db9944108760cf528dd695c1,

title = "Nuclear delivery of oligonucleotides via nanocomposites based on TiO2 nanoparticles and polylysine",

abstract = "The nuclear delivery of nucleic acid derivatives is an essential prerequisite for successful antisense therapy. Using laser confocal and electron microscopy, we have studied the uptake of fluorescently labeled oligonucleotides in the form of nanocomposites with polylysine and TiO2 nanoparticles into Caco2, MDCK, and HeLa cells. In all three cell lines, bright fluorescence has been detected after 30 min in the nuclei (excluding the nucleoli) of the interphase cells; no substantial increase in the intensity of the signal was observed for next 24 hours. In all cells undergoing mitosis, the signal was localized in the cytoplasm with zones of higher intensity around chromatin. In some cells, at the beginning of interphase (G-1 phase), fluorescence was not detected at all. The latter may be explained by the brief moment in the cell cycle when oligonucleotides delivered in the nanocomposite cannot be taken up by cells. The studied nanocomposites are prone to aggregation. The degree of aggregation increases with the storage time up to complete loss of the ability of the nanocomposites to penetrate the cells.",

keywords = "cell delivery, deoxyribozyme (DNAzyme), electron microscopy, scanning confocal laser microscopy, Animals, Caco-2 Cells, Cell Nucleus/metabolism, Dogs, Drug Delivery Systems/methods, HeLa Cells, Humans, Madin Darby Canine Kidney Cells, Nanocomposites/chemistry, Oligonucleotides/pharmacokinetics, Polylysine/chemistry, Titanium/chemistry",

author = "Chelobanov, {B. P.} and Repkova, {M. N.} and Baiborodin, {S. I.} and Ryabchikova, {E. I.} and Stetsenko, {D. A.}",

year = "2017",

month = nov,

day = "9",

doi = "10.7868/S0026898417050068",

language = "English",

volume = "51",

pages = "797--808",

journal = "Molekulyarnaya Biologiya",

issn = "0026-8984",

publisher = "Russian Academy of Sciences",

number = "5",

}

RIS

TY - JOUR

T1 - Nuclear delivery of oligonucleotides via nanocomposites based on TiO2 nanoparticles and polylysine

AU - Chelobanov, B. P.

AU - Repkova, M. N.

AU - Baiborodin, S. I.

AU - Ryabchikova, E. I.

AU - Stetsenko, D. A.

PY - 2017/11/9

Y1 - 2017/11/9

N2 - The nuclear delivery of nucleic acid derivatives is an essential prerequisite for successful antisense therapy. Using laser confocal and electron microscopy, we have studied the uptake of fluorescently labeled oligonucleotides in the form of nanocomposites with polylysine and TiO2 nanoparticles into Caco2, MDCK, and HeLa cells. In all three cell lines, bright fluorescence has been detected after 30 min in the nuclei (excluding the nucleoli) of the interphase cells; no substantial increase in the intensity of the signal was observed for next 24 hours. In all cells undergoing mitosis, the signal was localized in the cytoplasm with zones of higher intensity around chromatin. In some cells, at the beginning of interphase (G-1 phase), fluorescence was not detected at all. The latter may be explained by the brief moment in the cell cycle when oligonucleotides delivered in the nanocomposite cannot be taken up by cells. The studied nanocomposites are prone to aggregation. The degree of aggregation increases with the storage time up to complete loss of the ability of the nanocomposites to penetrate the cells.

AB - The nuclear delivery of nucleic acid derivatives is an essential prerequisite for successful antisense therapy. Using laser confocal and electron microscopy, we have studied the uptake of fluorescently labeled oligonucleotides in the form of nanocomposites with polylysine and TiO2 nanoparticles into Caco2, MDCK, and HeLa cells. In all three cell lines, bright fluorescence has been detected after 30 min in the nuclei (excluding the nucleoli) of the interphase cells; no substantial increase in the intensity of the signal was observed for next 24 hours. In all cells undergoing mitosis, the signal was localized in the cytoplasm with zones of higher intensity around chromatin. In some cells, at the beginning of interphase (G-1 phase), fluorescence was not detected at all. The latter may be explained by the brief moment in the cell cycle when oligonucleotides delivered in the nanocomposite cannot be taken up by cells. The studied nanocomposites are prone to aggregation. The degree of aggregation increases with the storage time up to complete loss of the ability of the nanocomposites to penetrate the cells.

KW - cell delivery

KW - deoxyribozyme (DNAzyme)

KW - electron microscopy

KW - scanning confocal laser microscopy

KW - Animals

KW - Caco-2 Cells

KW - Cell Nucleus/metabolism

KW - Dogs

KW - Drug Delivery Systems/methods

KW - HeLa Cells

KW - Humans

KW - Madin Darby Canine Kidney Cells

KW - Nanocomposites/chemistry

KW - Oligonucleotides/pharmacokinetics

KW - Polylysine/chemistry

KW - Titanium/chemistry

UR - http://www.scopus.com/inward/record.url?scp=85049090134&partnerID=8YFLogxK

U2 - 10.7868/S0026898417050068

DO - 10.7868/S0026898417050068

M3 - Article

C2 - 29116066

AN - SCOPUS:85049090134

VL - 51

SP - 797

EP - 808

JO - Molekulyarnaya Biologiya

JF - Molekulyarnaya Biologiya

SN - 0026-8984

IS - 5

ER -

ID: 14279754