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Novel peptide conjugates of modified oligonucleotides for inhibition of bacterial RNase P. / Novopashina, Darya; Vorobyeva, Mariya; Nazarov, Anton et al.

In: Frontiers in Pharmacology, Vol. 10, 813, 19.07.2019.

Research output: Contribution to journal › Article › peer-review

Harvard

Novopashina, D, Vorobyeva, M, Nazarov, A, Davydova, A, Danilin, N, Koroleva, L, Matveev, A, Bardasheva, A, Tikunova, N, Kupryushkin, M, Pyshnyi, D, Altman, S & Venyaminova, A 2019, 'Novel peptide conjugates of modified oligonucleotides for inhibition of bacterial RNase P', Frontiers in Pharmacology, vol. 10, 813. https://doi.org/10.3389/fphar.2019.00813

APA

Novopashina, D., Vorobyeva, M., Nazarov, A., Davydova, A., Danilin, N., Koroleva, L., Matveev, A., Bardasheva, A., Tikunova, N., Kupryushkin, M., Pyshnyi, D., Altman, S., & Venyaminova, A. (2019). Novel peptide conjugates of modified oligonucleotides for inhibition of bacterial RNase P. Frontiers in Pharmacology, 10, [813]. https://doi.org/10.3389/fphar.2019.00813

Vancouver

Novopashina D, Vorobyeva M, Nazarov A, Davydova A, Danilin N, Koroleva L et al. Novel peptide conjugates of modified oligonucleotides for inhibition of bacterial RNase P. Frontiers in Pharmacology. 2019 Jul 19;10:813. doi: 10.3389/fphar.2019.00813

Author

Novopashina, Darya ; Vorobyeva, Mariya ; Nazarov, Anton et al. / Novel peptide conjugates of modified oligonucleotides for inhibition of bacterial RNase P. In: Frontiers in Pharmacology. 2019 ; Vol. 10.

BibTeX

@article{76fa8ecdcae64d4ea87d2feaaedf6a07,
title = "Novel peptide conjugates of modified oligonucleotides for inhibition of bacterial RNase P",
abstract = "Novel alternatives to traditional antibiotics are now of great demand for the successful treatment of microbial infections. Here, we present the engineering and properties of new oligonucleotide inhibitors of RNase P, an essential bacterial enzyme. The series of 2{\textquoteright}-O-methyl RNA (2{\textquoteright}-OMe-RNA) and phosphoryl guanidine oligonucleotides were targeted to the substrate-binding region of M1 RNA subunit of the RNase P. Uniformly modified 2{\textquoteright}-OMe RNA and selectively modified phosphoryl guanidine oligonucleotides possessed good stability in biological media and effectively inhibited RNase P. Their conjugates with transporting peptides were shown to penetrate bacterial cells (Escherichia coli and Acinetobacter baumannii) and inhibit bacterial growth.",
keywords = "Antibacterial activity, Bacterial RNase P, Inhibition of RNase P, Modified oligonucleotides, Oligo(2{\textquoteright}-O-methylribonucleotides), Penetration into bacterial cells, Peptide conjugates of oligonucleotides, Phosphoryl guanidine oligonucleotides",
author = "Darya Novopashina and Mariya Vorobyeva and Anton Nazarov and Anna Davydova and Nikolay Danilin and Lyudmila Koroleva and Andrey Matveev and Alevtina Bardasheva and Nina Tikunova and Maxim Kupryushkin and Dmitrii Pyshnyi and Sidney Altman and Alya Venyaminova",
note = "Publisher Copyright: {\textcopyright} 2019 Novopashina, Vorobyeva, Nazarov, Davydova, Danilin, Koroleva, Matveev, Bardasheva, Tikunova, Kupryushkin, Pyshnyi, Altman and Venyaminova.",
year = "2019",
month = jul,
day = "19",
doi = "10.3389/fphar.2019.00813",
language = "English",
volume = "10",
journal = "Frontiers in Pharmacology",
issn = "1663-9812",
publisher = "Frontiers Media S.A.",

}

RIS

TY - JOUR

T1 - Novel peptide conjugates of modified oligonucleotides for inhibition of bacterial RNase P

AU - Novopashina, Darya

AU - Vorobyeva, Mariya

AU - Nazarov, Anton

AU - Davydova, Anna

AU - Danilin, Nikolay

AU - Koroleva, Lyudmila

AU - Matveev, Andrey

AU - Bardasheva, Alevtina

AU - Tikunova, Nina

AU - Kupryushkin, Maxim

AU - Pyshnyi, Dmitrii

AU - Altman, Sidney

AU - Venyaminova, Alya

N1 - Publisher Copyright: © 2019 Novopashina, Vorobyeva, Nazarov, Davydova, Danilin, Koroleva, Matveev, Bardasheva, Tikunova, Kupryushkin, Pyshnyi, Altman and Venyaminova.

PY - 2019/7/19

Y1 - 2019/7/19

N2 - Novel alternatives to traditional antibiotics are now of great demand for the successful treatment of microbial infections. Here, we present the engineering and properties of new oligonucleotide inhibitors of RNase P, an essential bacterial enzyme. The series of 2’-O-methyl RNA (2’-OMe-RNA) and phosphoryl guanidine oligonucleotides were targeted to the substrate-binding region of M1 RNA subunit of the RNase P. Uniformly modified 2’-OMe RNA and selectively modified phosphoryl guanidine oligonucleotides possessed good stability in biological media and effectively inhibited RNase P. Their conjugates with transporting peptides were shown to penetrate bacterial cells (Escherichia coli and Acinetobacter baumannii) and inhibit bacterial growth.

AB - Novel alternatives to traditional antibiotics are now of great demand for the successful treatment of microbial infections. Here, we present the engineering and properties of new oligonucleotide inhibitors of RNase P, an essential bacterial enzyme. The series of 2’-O-methyl RNA (2’-OMe-RNA) and phosphoryl guanidine oligonucleotides were targeted to the substrate-binding region of M1 RNA subunit of the RNase P. Uniformly modified 2’-OMe RNA and selectively modified phosphoryl guanidine oligonucleotides possessed good stability in biological media and effectively inhibited RNase P. Their conjugates with transporting peptides were shown to penetrate bacterial cells (Escherichia coli and Acinetobacter baumannii) and inhibit bacterial growth.

KW - Antibacterial activity

KW - Bacterial RNase P

KW - Inhibition of RNase P

KW - Modified oligonucleotides

KW - Oligo(2’-O-methylribonucleotides)

KW - Penetration into bacterial cells

KW - Peptide conjugates of oligonucleotides

KW - Phosphoryl guanidine oligonucleotides

UR - http://www.scopus.com/inward/record.url?scp=85083969058&partnerID=8YFLogxK

U2 - 10.3389/fphar.2019.00813

DO - 10.3389/fphar.2019.00813

M3 - Article

C2 - 31379580

VL - 10

JO - Frontiers in Pharmacology

JF - Frontiers in Pharmacology

SN - 1663-9812

M1 - 813

ER -

ID: 23290974