Novel approach to the delivery into the cells and light-activation of the guide RNA for the genome editing CRISPR/Cas9 system. / Яковлев, А.; Ахметова, Е.; Данилин, Николай Александрович et al.
2021. 122 Abstract from FEBS 2021. The 45th FEBS Congress.Research output: Contribution to conference › Abstract › peer-review
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TY - CONF
T1 - Novel approach to the delivery into the cells and light-activation of the guide RNA for the genome editing CRISPR/Cas9 system
AU - Яковлев, А.
AU - Ахметова, Е.
AU - Данилин, Николай Александрович
AU - Семиколенова, Ольга Андреевна
AU - Вохтанцев, Иван Павлович
AU - Ким, Дарья Вячеславовна
AU - Жарков, Дмитрий Олегович
AU - Веньяминова, Алия Гусейновна
AU - Новопашина, Дарья Сергеевна
N1 - The reported study was funded by RFBR, project number 19-34-51026.
PY - 2021/7
Y1 - 2021/7
N2 - Use of the CRISPR/Cas9 system for molecular biological and gene engineering purposes is the issue of the day. Effective delivery of the components of system in cells is obligatory for their successful application. The main goal of our study is the development of novel approach for the delivery of guide RNA (crRNA) in the cells. The proposed approach is based on the usage of additional photocleavable oligodeoxyribonucleotide complementary to guide RNA and bearing ligands facilitating the penetration of the whole construction through the cell membrane.
AB - Use of the CRISPR/Cas9 system for molecular biological and gene engineering purposes is the issue of the day. Effective delivery of the components of system in cells is obligatory for their successful application. The main goal of our study is the development of novel approach for the delivery of guide RNA (crRNA) in the cells. The proposed approach is based on the usage of additional photocleavable oligodeoxyribonucleotide complementary to guide RNA and bearing ligands facilitating the penetration of the whole construction through the cell membrane.
M3 - Abstract
SP - 122
T2 - FEBS 2021. The 45th FEBS Congress
Y2 - 3 July 2021 through 8 July 2021
ER -
ID: 35168149