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Normal vibrations of ternary DOPC/DPPC/cholesterol lipid bilayers by low-frequency Raman spectroscopy. / Leonov, Dmitry V.; Dzuba, Sergei A.; Surovtsev, Nikolay V.

In: RSC Advances, Vol. 9, No. 59, 27.10.2019, p. 34451-34456.

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@article{7a88b915d7104e39991750389627e510,
title = "Normal vibrations of ternary DOPC/DPPC/cholesterol lipid bilayers by low-frequency Raman spectroscopy",
abstract = "A lipid bilayer containing a ternary mixture of low- A nd high-melting lipids and cholesterol (Chol) can give rise to domain formation, referred to as lipid rafts. Low-frequency Raman spectroscopy at reduced temperatures allows detection of normal membrane mechanical vibrations. In this work, Raman spectra were obtained in the spectral range between 5 and 90 cm-1 for bilayers prepared from dioleoyl-glycero-phosphocholine (DOPC), dipalmitoyl-glycero-phosphocholine (DPPC) and Chol. A narrow peak detected between 13 and 16 cm-1 was attributed to the vibrational eigenmode of a lipid monolayer (a leaflet). For the equimolar DOPC/DPPC ratio, the Chol concentration dependence for the peak position, width and amplitude may be divided into three distinct ranges: Below 9 mol%, the intermediate range between 9 mol% and 38 mol%, and above 38 mol%. In the intermediate range the peak position attains its minimum, and the peak width drops approximately by a factor of two as compared with the Chol-free bilayers. Meanwhile, this range is known for raft formation in a fluid state. The obtained results may be interpreted as evidence that bilayer structures in the raft-containing fluid state may be frozen at low temperatures. The drop of peak width indicates that at the spatial scale of the experiment (∼2.5 nm) the intermolecular bilayer structure with raft formation becomes more homogeneous and more cohesive.",
keywords = "MODEL MEMBRANES, PHASE-DIAGRAM, DYNAMICS, RAFTS, PHOSPHATIDYLCHOLINE, FLUCTUATIONS",
author = "Leonov, {Dmitry V.} and Dzuba, {Sergei A.} and Surovtsev, {Nikolay V.}",
note = "This journal is {\textcopyright} The Royal Society of Chemistry.",
year = "2019",
month = oct,
day = "27",
doi = "10.1039/c9ra06114b",
language = "English",
volume = "9",
pages = "34451--34456",
journal = "RSC Advances",
issn = "2046-2069",
publisher = "ROYAL SOC CHEMISTRY",
number = "59",

}

RIS

TY - JOUR

T1 - Normal vibrations of ternary DOPC/DPPC/cholesterol lipid bilayers by low-frequency Raman spectroscopy

AU - Leonov, Dmitry V.

AU - Dzuba, Sergei A.

AU - Surovtsev, Nikolay V.

N1 - This journal is © The Royal Society of Chemistry.

PY - 2019/10/27

Y1 - 2019/10/27

N2 - A lipid bilayer containing a ternary mixture of low- A nd high-melting lipids and cholesterol (Chol) can give rise to domain formation, referred to as lipid rafts. Low-frequency Raman spectroscopy at reduced temperatures allows detection of normal membrane mechanical vibrations. In this work, Raman spectra were obtained in the spectral range between 5 and 90 cm-1 for bilayers prepared from dioleoyl-glycero-phosphocholine (DOPC), dipalmitoyl-glycero-phosphocholine (DPPC) and Chol. A narrow peak detected between 13 and 16 cm-1 was attributed to the vibrational eigenmode of a lipid monolayer (a leaflet). For the equimolar DOPC/DPPC ratio, the Chol concentration dependence for the peak position, width and amplitude may be divided into three distinct ranges: Below 9 mol%, the intermediate range between 9 mol% and 38 mol%, and above 38 mol%. In the intermediate range the peak position attains its minimum, and the peak width drops approximately by a factor of two as compared with the Chol-free bilayers. Meanwhile, this range is known for raft formation in a fluid state. The obtained results may be interpreted as evidence that bilayer structures in the raft-containing fluid state may be frozen at low temperatures. The drop of peak width indicates that at the spatial scale of the experiment (∼2.5 nm) the intermolecular bilayer structure with raft formation becomes more homogeneous and more cohesive.

AB - A lipid bilayer containing a ternary mixture of low- A nd high-melting lipids and cholesterol (Chol) can give rise to domain formation, referred to as lipid rafts. Low-frequency Raman spectroscopy at reduced temperatures allows detection of normal membrane mechanical vibrations. In this work, Raman spectra were obtained in the spectral range between 5 and 90 cm-1 for bilayers prepared from dioleoyl-glycero-phosphocholine (DOPC), dipalmitoyl-glycero-phosphocholine (DPPC) and Chol. A narrow peak detected between 13 and 16 cm-1 was attributed to the vibrational eigenmode of a lipid monolayer (a leaflet). For the equimolar DOPC/DPPC ratio, the Chol concentration dependence for the peak position, width and amplitude may be divided into three distinct ranges: Below 9 mol%, the intermediate range between 9 mol% and 38 mol%, and above 38 mol%. In the intermediate range the peak position attains its minimum, and the peak width drops approximately by a factor of two as compared with the Chol-free bilayers. Meanwhile, this range is known for raft formation in a fluid state. The obtained results may be interpreted as evidence that bilayer structures in the raft-containing fluid state may be frozen at low temperatures. The drop of peak width indicates that at the spatial scale of the experiment (∼2.5 nm) the intermolecular bilayer structure with raft formation becomes more homogeneous and more cohesive.

KW - MODEL MEMBRANES

KW - PHASE-DIAGRAM

KW - DYNAMICS

KW - RAFTS

KW - PHOSPHATIDYLCHOLINE

KW - FLUCTUATIONS

UR - http://www.scopus.com/inward/record.url?scp=85074542997&partnerID=8YFLogxK

U2 - 10.1039/c9ra06114b

DO - 10.1039/c9ra06114b

M3 - Article

C2 - 35530012

AN - SCOPUS:85074542997

VL - 9

SP - 34451

EP - 34456

JO - RSC Advances

JF - RSC Advances

SN - 2046-2069

IS - 59

ER -

ID: 22320827