Standard

New Two-Component Pyrene Probes Based on Oligo(2'-O-Methylribonucleotides) for microRNA Detection. / Semikolenova, O. A.; Golyshev, V. M.; Kim, B. H. et al.

In: Russian Journal of Bioorganic Chemistry, Vol. 47, No. 2, 03.2021, p. 432-440.

Research output: Contribution to journal › Article › peer-review

Harvard

Semikolenova, OA, Golyshev, VM, Kim, BH, Venyaminova, AG & Novopashina, DS 2021, 'New Two-Component Pyrene Probes Based on Oligo(2'-O-Methylribonucleotides) for microRNA Detection', Russian Journal of Bioorganic Chemistry, vol. 47, no. 2, pp. 432-440. https://doi.org/10.1134/S1068162021020230

APA

Semikolenova, O. A., Golyshev, V. M., Kim, B. H., Venyaminova, A. G., & Novopashina, D. S. (2021). New Two-Component Pyrene Probes Based on Oligo(2'-O-Methylribonucleotides) for microRNA Detection. Russian Journal of Bioorganic Chemistry, 47(2), 432-440. https://doi.org/10.1134/S1068162021020230

Vancouver

Semikolenova OA, Golyshev VM, Kim BH, Venyaminova AG, Novopashina DS. New Two-Component Pyrene Probes Based on Oligo(2'-O-Methylribonucleotides) for microRNA Detection. Russian Journal of Bioorganic Chemistry. 2021 Mar;47(2):432-440. doi: 10.1134/S1068162021020230

Author

Semikolenova, O. A. ; Golyshev, V. M. ; Kim, B. H. et al. / New Two-Component Pyrene Probes Based on Oligo(2'-O-Methylribonucleotides) for microRNA Detection. In: Russian Journal of Bioorganic Chemistry. 2021 ; Vol. 47, No. 2. pp. 432-440.

BibTeX

@article{918e0640fe43427193d83b0d7e4e1145,
title = "New Two-Component Pyrene Probes Based on Oligo(2'-O-Methylribonucleotides) for microRNA Detection",
abstract = "New two-component pyrene probes based on oligo(2'-O-methylribonucleotides) for microRNA detection have been designed. They contain the PyA-modified adenine cluster (pentaadenosine fragment that contains 8-(1-ethynylpyrene)-deoxyriboadenosine in the center) and can form a three-way junction (3WJ) structure with a target RNA. We have chosen microRNA let-7a-3p as the RNA target because of the correlation of its concentration in cells with the appearance and progression of cancer. We have compared the thermal stability and fluorescence properties of the two-component probes based on oligo(2'-O-methylribonucleotides) that contain either deoxyriboadenosine (dAdAPyAdAdA) or the (2'-O-methylribo)adenosine (AmAm PyAAmAm) cluster with those of (dAdAPyAdAdA)-containing oligodeoxyribonucleotide. The changes in the fluorescence spectra of two-component probes after hybridization with the RNA target have been demonstrated. These probes can be used for designing a new microRNA detection system.",
keywords = "A-modified adenine cluster, 3WJ structure, 8-(1-ethynylpyrene)-deoxyriboadenosine, fluorescent properties, oligo(2'-O-methylribonucleotides), RNA detection",
author = "Semikolenova, {O. A.} and Golyshev, {V. M.} and Kim, {B. H.} and Venyaminova, {Aliya G.} and Novopashina, {D. S.}",
note = "Funding Information: The work was supported by the State-funded budget projects (Program of fundamental scientific research of the State Academies of Sciences for 2013–2020 [АААА-А17-117020210021-7]). Publisher Copyright: {\textcopyright} 2021, Pleiades Publishing, Ltd. Copyright: Copyright 2021 Elsevier B.V., All rights reserved.",
year = "2021",
month = mar,
doi = "10.1134/S1068162021020230",
language = "English",
volume = "47",
pages = "432--440",
journal = "Russian Journal of Bioorganic Chemistry",
issn = "1068-1620",
publisher = "MAIK NAUKA/INTERPERIODICA/SPRINGER",
number = "2",

}

RIS

TY - JOUR

T1 - New Two-Component Pyrene Probes Based on Oligo(2'-O-Methylribonucleotides) for microRNA Detection

AU - Semikolenova, O. A.

AU - Golyshev, V. M.

AU - Kim, B. H.

AU - Venyaminova, Aliya G.

AU - Novopashina, D. S.

N1 - Funding Information: The work was supported by the State-funded budget projects (Program of fundamental scientific research of the State Academies of Sciences for 2013–2020 [АААА-А17-117020210021-7]). Publisher Copyright: © 2021, Pleiades Publishing, Ltd. Copyright: Copyright 2021 Elsevier B.V., All rights reserved.

PY - 2021/3

Y1 - 2021/3

N2 - New two-component pyrene probes based on oligo(2'-O-methylribonucleotides) for microRNA detection have been designed. They contain the PyA-modified adenine cluster (pentaadenosine fragment that contains 8-(1-ethynylpyrene)-deoxyriboadenosine in the center) and can form a three-way junction (3WJ) structure with a target RNA. We have chosen microRNA let-7a-3p as the RNA target because of the correlation of its concentration in cells with the appearance and progression of cancer. We have compared the thermal stability and fluorescence properties of the two-component probes based on oligo(2'-O-methylribonucleotides) that contain either deoxyriboadenosine (dAdAPyAdAdA) or the (2'-O-methylribo)adenosine (AmAm PyAAmAm) cluster with those of (dAdAPyAdAdA)-containing oligodeoxyribonucleotide. The changes in the fluorescence spectra of two-component probes after hybridization with the RNA target have been demonstrated. These probes can be used for designing a new microRNA detection system.

AB - New two-component pyrene probes based on oligo(2'-O-methylribonucleotides) for microRNA detection have been designed. They contain the PyA-modified adenine cluster (pentaadenosine fragment that contains 8-(1-ethynylpyrene)-deoxyriboadenosine in the center) and can form a three-way junction (3WJ) structure with a target RNA. We have chosen microRNA let-7a-3p as the RNA target because of the correlation of its concentration in cells with the appearance and progression of cancer. We have compared the thermal stability and fluorescence properties of the two-component probes based on oligo(2'-O-methylribonucleotides) that contain either deoxyriboadenosine (dAdAPyAdAdA) or the (2'-O-methylribo)adenosine (AmAm PyAAmAm) cluster with those of (dAdAPyAdAdA)-containing oligodeoxyribonucleotide. The changes in the fluorescence spectra of two-component probes after hybridization with the RNA target have been demonstrated. These probes can be used for designing a new microRNA detection system.

KW - A-modified adenine cluster

KW - 3WJ structure

KW - 8-(1-ethynylpyrene)-deoxyriboadenosine

KW - fluorescent properties

KW - oligo(2'-O-methylribonucleotides)

KW - RNA detection

UR - http://www.scopus.com/inward/record.url?scp=85104822304&partnerID=8YFLogxK

U2 - 10.1134/S1068162021020230

DO - 10.1134/S1068162021020230

M3 - Article

AN - SCOPUS:85104822304

VL - 47

SP - 432

EP - 440

JO - Russian Journal of Bioorganic Chemistry

JF - Russian Journal of Bioorganic Chemistry

SN - 1068-1620

IS - 2

ER -

ID: 28452661