TY - JOUR

T1 - New slbo-Gal4 driver lines for the analysis of border cell migration during Drosophila oogenesis

AU - Ogienko, Anna A.

AU - Yarinich, Lyubov A.

AU - Fedorova, Elena V.

AU - Lebedev, Mikhail O.

AU - Andreyeva, Evgeniya N.

AU - Pindyurin, Alexey V.

AU - Baricheva, Elina M.

N1 - Publisher Copyright: © 2018, Springer-Verlag GmbH Germany, part of Springer Nature.

PY - 2018/12/1

Y1 - 2018/12/1

N2 - Border cell (BC) migration during Drosophila oogenesis is an excellent model for the analysis of the migratory and invasive cell behavior. Most studies on BC migration have exploited a slbo-Gal4 driver to regulate gene expression in these cells or to mark them. Here, we report that the slbo-Gal4 transgene present in the line #6458 from the Bloomington Stock Center is inserted within chickadee (chic), a gene encoding the actin-binding protein Profilin, which promotes actin polymerization and is known to be involved in cell migration. The chic6458 mutation caused by the transgene insertion behaves as a null chic allele and is homozygous lethal. To evaluate possible effects of chic6458 on the assessment of BC behavior, we generated new lines bearing the slbo-Gal4 transgene inserted into different second chromosome loci that do not appear to be involved in cell migration. Using these new lines and the slbo-Gal4-chic6458 line, we defined the functional relationships between the twinfilin (twf) and chic in BC migration. Migration of BCs is substantially reduced by mutations in twf, which encodes an actin-binding protein that inhibits actin filament assembly. The defects caused by twf mutations are significantly suppressed when the slbo-Gal4-chic6458, but not the new slbo-Gal4 drivers were used. These findings indicate twf and chic interact and function antagonistically during BC migration in Drosophila oogenesis.

AB - Border cell (BC) migration during Drosophila oogenesis is an excellent model for the analysis of the migratory and invasive cell behavior. Most studies on BC migration have exploited a slbo-Gal4 driver to regulate gene expression in these cells or to mark them. Here, we report that the slbo-Gal4 transgene present in the line #6458 from the Bloomington Stock Center is inserted within chickadee (chic), a gene encoding the actin-binding protein Profilin, which promotes actin polymerization and is known to be involved in cell migration. The chic6458 mutation caused by the transgene insertion behaves as a null chic allele and is homozygous lethal. To evaluate possible effects of chic6458 on the assessment of BC behavior, we generated new lines bearing the slbo-Gal4 transgene inserted into different second chromosome loci that do not appear to be involved in cell migration. Using these new lines and the slbo-Gal4-chic6458 line, we defined the functional relationships between the twinfilin (twf) and chic in BC migration. Migration of BCs is substantially reduced by mutations in twf, which encodes an actin-binding protein that inhibits actin filament assembly. The defects caused by twf mutations are significantly suppressed when the slbo-Gal4-chic6458, but not the new slbo-Gal4 drivers were used. These findings indicate twf and chic interact and function antagonistically during BC migration in Drosophila oogenesis.

KW - BDSC line #6458

KW - BDSC line #76363

KW - Border cells

KW - Cell migration

KW - chickadee

KW - Drosophila melanogaster

KW - Profilin

KW - slbo-Gal4 driver

KW - twinfilin

KW - ENCODES

KW - PROTEIN

KW - CORTACTIN

KW - MODEL

KW - FATE

KW - MORPHOGENESIS

KW - GENES

KW - EXPRESSION

KW - TWINFILIN

KW - MOTILITY

UR - http://www.scopus.com/inward/record.url?scp=85050287585&partnerID=8YFLogxK

U2 - 10.1007/s00412-018-0676-7

DO - 10.1007/s00412-018-0676-7

M3 - Article

C2 - 30030602

AN - SCOPUS:85050287585

VL - 127

SP - 475

EP - 487

JO - Chromosoma

JF - Chromosoma

SN - 0009-5915

IS - 4

ER -