Research output: Contribution to journal › Article › peer-review
New Fluorescent Analogs of Nucleotides Based on 3-Hydroxychromone for Recording Conformational Changes of DNA. / Kladova, O. A.; Kuznetsova, A. A.; Barthes, Nicolas P.F. et al.
In: Russian Journal of Bioorganic Chemistry, Vol. 45, No. 6, 01.11.2019, p. 599-607.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - New Fluorescent Analogs of Nucleotides Based on 3-Hydroxychromone for Recording Conformational Changes of DNA
AU - Kladova, O. A.
AU - Kuznetsova, A. A.
AU - Barthes, Nicolas P.F.
AU - Michel, Benoit Y.
AU - Burger, Alain
AU - Fedorova, O. S.
AU - Kuznetsov, N. A.
PY - 2019/11/1
Y1 - 2019/11/1
N2 - It has recently been found that derivatives of nucleotides containing а 3-hydroxychromone fluorescent dye can be used as sensitive markers of conformational changes of DNA. In this work, a comparative analysis of two fluorescent nucleotide derivatives—3-hydroxychromone a (3HC) and 3HC-modified uridine (FCU)—was performed during the study of protein–nucleic acid interactions for several human DNA repair enzymes, removing damaged nucleotides: DNA glycosylases AAG, OGG1, UNG2, and MBD4 and AP endonuclease APE1. The changes of fluorescence intensity significantly depended on the nature of neighbor nucleotides and may be opposite in direction for different cases. The FCU residue located in the complementary strand opposite to damaged nucleotide or in the same strand moved by few nucleotides, is very sensitive to processes induced by DNA glycosylases in the course of formation of enzyme–substrate complexes, which include local melting and bending of the DNA chain, as well as eversion of the damaged nucleotide from DNA double helix and insertion of amino acids of the active site into the void.
AB - It has recently been found that derivatives of nucleotides containing а 3-hydroxychromone fluorescent dye can be used as sensitive markers of conformational changes of DNA. In this work, a comparative analysis of two fluorescent nucleotide derivatives—3-hydroxychromone a (3HC) and 3HC-modified uridine (FCU)—was performed during the study of protein–nucleic acid interactions for several human DNA repair enzymes, removing damaged nucleotides: DNA glycosylases AAG, OGG1, UNG2, and MBD4 and AP endonuclease APE1. The changes of fluorescence intensity significantly depended on the nature of neighbor nucleotides and may be opposite in direction for different cases. The FCU residue located in the complementary strand opposite to damaged nucleotide or in the same strand moved by few nucleotides, is very sensitive to processes induced by DNA glycosylases in the course of formation of enzyme–substrate complexes, which include local melting and bending of the DNA chain, as well as eversion of the damaged nucleotide from DNA double helix and insertion of amino acids of the active site into the void.
KW - AP endonuclease
KW - conformational changes
KW - damage repair
KW - DNA
KW - DNA glycosylase
KW - enzyme kinetics
KW - fluorescence
KW - GLYCOSYLASE
KW - CRYSTAL-STRUCTURE
KW - ABASIC DNA
KW - SUBSTRATE RECOGNITION
KW - NUCLEIC-ACID STRUCTURE
KW - REPAIR
KW - DAMAGE RECOGNITION
KW - KINETIC-ANALYSIS
KW - DYNAMICS
KW - BINDING
UR - http://www.scopus.com/inward/record.url?scp=85078626891&partnerID=8YFLogxK
U2 - 10.1134/S1068162019060220
DO - 10.1134/S1068162019060220
M3 - Article
AN - SCOPUS:85078626891
VL - 45
SP - 599
EP - 607
JO - Russian Journal of Bioorganic Chemistry
JF - Russian Journal of Bioorganic Chemistry
SN - 1068-1620
IS - 6
ER -
ID: 23287278