TY - JOUR

T1 - New Data on Organization and Spatial Localization of B-Chromosomes in Cell Nuclei of the Yellow-Necked Mouse Apodemus flavicollis

AU - Karamysheva, Tatyana

AU - Romanenko, Svetlana

AU - Makunin, Alexey

AU - Rajičić, Marija

AU - Bogdanov, Alexey

AU - Trifonov, Vladimir

AU - Blagojević, Jelena

AU - Vujošević, Mladen

AU - Orishchenko, Konstantin

AU - Rubtsov, Nikolay

N1 - This study was supported by the Russian Foundation for Basic Research (#19-015-00084a) in the part of chromosomal microdissection and the State Budget of ICG and by the Ministry of Science and Higher Education of the Russian Federation via IC&G SB RAS (#0259-2021-0011) in the part of FISH analyses. Material collecting and treatment were partly supported by Government basic research program No. 0088-2021-0019 (Koltzov Institute of Developmental Biology RAS, Moscow, Russia). Confocal microscopy and data analysis were performed with support from the Ministry of Science and Higher Education of the Russian Federation via the Novosibirsk State University (#2019-0546 (FSUS-2020-0040)). The cytogenetic analysis was supported by the Wellcome Trust grant 206194. Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland.

PY - 2021/7/19

Y1 - 2021/7/19

N2 - The gene composition, function and evolution of B-chromosomes (Bs) have been actively discussed in recent years. However, the additional genomic elements are still enigmatic. One of Bs mysteries is their spatial organization in the interphase nucleus. It is known that heterochromatic compartments are not randomly localized in a nucleus. The purpose of this work was to study the organization and three-dimensional spatial arrangement of Bs in the interphase nucleus. Using microdissection of Bs and autosome centromeric heterochromatic regions of the yellow-necked mouse (Apodemus flavicollis) we obtained DNA probes for further two-dimensional (2D)- and three-dimensional (3D)- fluorescence in situ hybridization (FISH) studies. Simultaneous in situ hybridization of obtained here B-specific DNA probes and autosomal C-positive pericentromeric region-specific probes further corroborated the previously stated hypothesis about the pseudoautosomal origin of the additional chromosomes of this species. Analysis of the spatial organization of the Bs demonstrated the peripheral location of B-specific chromatin within the interphase nucleus and feasible contact with the nuclear envelope (similarly to pericentromeric regions of autosomes and sex chromosomes). It is assumed that such interaction is essential for the regulation of nuclear architecture. It also points out that Bs may follow the same mechanism as sex chromosomes to avoid a meiotic checkpoint.

AB - The gene composition, function and evolution of B-chromosomes (Bs) have been actively discussed in recent years. However, the additional genomic elements are still enigmatic. One of Bs mysteries is their spatial organization in the interphase nucleus. It is known that heterochromatic compartments are not randomly localized in a nucleus. The purpose of this work was to study the organization and three-dimensional spatial arrangement of Bs in the interphase nucleus. Using microdissection of Bs and autosome centromeric heterochromatic regions of the yellow-necked mouse (Apodemus flavicollis) we obtained DNA probes for further two-dimensional (2D)- and three-dimensional (3D)- fluorescence in situ hybridization (FISH) studies. Simultaneous in situ hybridization of obtained here B-specific DNA probes and autosomal C-positive pericentromeric region-specific probes further corroborated the previously stated hypothesis about the pseudoautosomal origin of the additional chromosomes of this species. Analysis of the spatial organization of the Bs demonstrated the peripheral location of B-specific chromatin within the interphase nucleus and feasible contact with the nuclear envelope (similarly to pericentromeric regions of autosomes and sex chromosomes). It is assumed that such interaction is essential for the regulation of nuclear architecture. It also points out that Bs may follow the same mechanism as sex chromosomes to avoid a meiotic checkpoint.

KW - 3D genome organization

KW - B-chromosomes (Bs)

KW - chromosome territories (CT)

KW - confocal microscopy

KW - duplication clusters

KW - microdissection

KW - repetitive DNA

KW - speciation

UR - http://www.scopus.com/inward/record.url?scp=85114069231&partnerID=8YFLogxK

U2 - 10.3390/cells10071819

DO - 10.3390/cells10071819

M3 - Article

C2 - 34359988

AN - SCOPUS:85114069231

VL - 10

JO - Cells

JF - Cells

SN - 2073-4409

IS - 7

M1 - 1819

ER -