Research output: Contribution to journal › Article › peer-review
Mutational and kinetic analysis of lesion recognition by Escherichia coli endonuclease VIII. / Kladova, Olga A.; Kuznetsova, Alexandra A.; Fedorova, Olga S. et al.
In: Genes, Vol. 8, No. 5, 140, 13.05.2017.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Mutational and kinetic analysis of lesion recognition by Escherichia coli endonuclease VIII
AU - Kladova, Olga A.
AU - Kuznetsova, Alexandra A.
AU - Fedorova, Olga S.
AU - Kuznetsov, Nikita A.
N1 - Publisher Copyright: © 2017 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2017/5/13
Y1 - 2017/5/13
N2 - Escherichia coli endonuclease VIII (Endo VIII) is a DNA glycosylase with substrate specificity for a wide range of oxidatively damaged pyrimidine bases. Endo VIII catalyzes hydrolysis of the N-glycosidic bond and β, δ-elimination of 3′ - and 5′ -phosphate groups of an apurinic/apyrimidinic site. Single mutants of Endo VIII L70S, L70W, Y71W, F121W, F230W, and P253W were analyzed here with the aim to elucidate the kinetic mechanism of protein conformational adjustment during damaged-nucleotide recognition and catalytic-complex formation. F121W substitution leads to a slight reduction of DNA binding and catalytic activity. F230W substitution slows the rate of the δ-elimination reaction indicating that interaction of Phe230 with a 5′ -phosphate group proceeds in the latest catalytic step. P253W Endo VIII has the same activity as the wild type (WT) enzyme. Y71W substitution slightly reduces the catalytic activity due to the effect on the later steps of catalytic-complex formation. Both L70S and L70W substitutions significantly decrease the catalytic activity, indicating that Leu70 plays an important role in the course of enzyme-DNA catalytic complex formation. Our data suggest that Leu70 forms contacts with DNA earlier than Tyr71 does. Therefore, most likely, Leu70 plays the role of a DNA lesion “sensor”, which is used by Endo VIII for recognition of a DNA damage site.
AB - Escherichia coli endonuclease VIII (Endo VIII) is a DNA glycosylase with substrate specificity for a wide range of oxidatively damaged pyrimidine bases. Endo VIII catalyzes hydrolysis of the N-glycosidic bond and β, δ-elimination of 3′ - and 5′ -phosphate groups of an apurinic/apyrimidinic site. Single mutants of Endo VIII L70S, L70W, Y71W, F121W, F230W, and P253W were analyzed here with the aim to elucidate the kinetic mechanism of protein conformational adjustment during damaged-nucleotide recognition and catalytic-complex formation. F121W substitution leads to a slight reduction of DNA binding and catalytic activity. F230W substitution slows the rate of the δ-elimination reaction indicating that interaction of Phe230 with a 5′ -phosphate group proceeds in the latest catalytic step. P253W Endo VIII has the same activity as the wild type (WT) enzyme. Y71W substitution slightly reduces the catalytic activity due to the effect on the later steps of catalytic-complex formation. Both L70S and L70W substitutions significantly decrease the catalytic activity, indicating that Leu70 plays an important role in the course of enzyme-DNA catalytic complex formation. Our data suggest that Leu70 forms contacts with DNA earlier than Tyr71 does. Therefore, most likely, Leu70 plays the role of a DNA lesion “sensor”, which is used by Endo VIII for recognition of a DNA damage site.
KW - Base excision repair
KW - Conformational dynamics
KW - DNA glycosylase
KW - Endonuclease VIII
KW - Stopped-flow enzyme kinetics
KW - ORTHOLOG
KW - FORMAMIDOPYRIMIDINE-DNA GLYCOSYLASE
KW - MECHANISM
KW - STEADY-STATE KINETICS
KW - SEARCH
KW - base excision repair
KW - stopped-flow enzyme kinetics
KW - CONFORMATIONAL DYNAMICS
KW - REPAIR
KW - DAMAGE RECOGNITION
KW - conformational dynamics
KW - FINGER
KW - endonuclease VIII
KW - REAL-TIME
UR - http://www.scopus.com/inward/record.url?scp=85019236248&partnerID=8YFLogxK
U2 - 10.3390/genes8050140
DO - 10.3390/genes8050140
M3 - Article
C2 - 28505099
AN - SCOPUS:85019236248
VL - 8
JO - Genes
JF - Genes
SN - 2073-4425
IS - 5
M1 - 140
ER -
ID: 8681278