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MPTP-Treated Zebrafish Recapitulate ‘Late-Stage’ Parkinson’s-like Cognitive Decline. / Bashirzade, Alim A.O.; Cheresiz, Sergey V.; Belova, Alisa S. et al.

In: Toxics, Vol. 10, No. 2, 69, 02.2022.

Research output: Contribution to journal › Article › peer-review

Harvard

Bashirzade, AAO, Cheresiz, SV, Belova, AS, Drobkov, AV, Korotaeva, AD, Azizi-Arani, S, Azimirad, A, Odle, E, Gild, EYV, Ardashov, OV, Volcho, KP, Bozhko, DV, Myrov, VO, Kolchanova, SM, Polovian, AI, Galumov, GK, Salakhutdinov, NF, Amstislavskaya, TG & Kalueff, AV 2022, 'MPTP-Treated Zebrafish Recapitulate ‘Late-Stage’ Parkinson’s-like Cognitive Decline', Toxics, vol. 10, no. 2, 69. https://doi.org/10.3390/toxics10020069

APA

Bashirzade, A. A. O., Cheresiz, S. V., Belova, A. S., Drobkov, A. V., Korotaeva, A. D., Azizi-Arani, S., Azimirad, A., Odle, E., Gild, E. Y. V., Ardashov, O. V., Volcho, K. P., Bozhko, D. V., Myrov, V. O., Kolchanova, S. M., Polovian, A. I., Galumov, G. K., Salakhutdinov, N. F., Amstislavskaya, T. G., & Kalueff, A. V. (2022). MPTP-Treated Zebrafish Recapitulate ‘Late-Stage’ Parkinson’s-like Cognitive Decline. Toxics, 10(2), [69]. https://doi.org/10.3390/toxics10020069

Vancouver

Bashirzade AAO, Cheresiz SV, Belova AS, Drobkov AV, Korotaeva AD, Azizi-Arani S et al. MPTP-Treated Zebrafish Recapitulate ‘Late-Stage’ Parkinson’s-like Cognitive Decline. Toxics. 2022 Feb;10(2):69. doi: 10.3390/toxics10020069

Author

Bashirzade, Alim A.O. ; Cheresiz, Sergey V. ; Belova, Alisa S. et al. / MPTP-Treated Zebrafish Recapitulate ‘Late-Stage’ Parkinson’s-like Cognitive Decline. In: Toxics. 2022 ; Vol. 10, No. 2.

BibTeX

@article{c6e32dcc3a0344b99dabe08660784bd7,
title = "MPTP-Treated Zebrafish Recapitulate {\textquoteleft}Late-Stage{\textquoteright} Parkinson{\textquoteright}s-like Cognitive Decline",
abstract = "The zebrafish is a promising model species in biomedical research, including neurotoxicology and neuroactive drug screening. 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) evokes degeneration of dopaminergic neurons and is commonly used to model Parkinson{\textquoteright}s disease (PD) in laboratory animals, including zebrafish. However, cognitive phenotypes in MPTP-evoked experimental PD models remain poorly understood. Here, we established an LD50 (292 mg/kg) for intraperitoneal MPTP administration in adult zebrafish, and report impaired spatial working memory (poorer spontaneous alternation in the Y-maze) in a PD model utilizing fish treated with 200 µg of this agent. In addition to conventional behavioral analyses, we also employed artificial intelligence (AI)-based approaches to independently and without bias characterize MPTP effects on zebrafish behavior during the Y-maze test. These analyses yielded a distinct cluster for 200-µg MPTP (vs. other) groups, suggesting that high-dose MPTP produced distinct, computationally detectable patterns of zebrafish swimming. Collectively, these findings support MPTP treatment in adult zebrafish as a late-stage experimental PD model with overt cognitive phenotypes.",
keywords = "1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP), Artificial intelligence, Inhibitory avoidance task, Parkinson{\textquoteright}s disease, Spontaneous alternation, Zebrafish",
author = "Bashirzade, {Alim A.O.} and Cheresiz, {Sergey V.} and Belova, {Alisa S.} and Drobkov, {Alexey V.} and Korotaeva, {Anastasiia D.} and Soheil Azizi-Arani and Amirhossein Azimirad and Eric Odle and Gild, {Emma Yanina V.} and Ardashov, {Oleg V.} and Volcho, {Konstantin P.} and Bozhko, {Dmitrii V.} and Myrov, {Vladislav O.} and Kolchanova, {Sofia M.} and Polovian, {Aleksander I.} and Galumov, {Georgii K.} and Salakhutdinov, {Nariman F.} and Amstislavskaya, {Tamara G.} and Kalueff, {Allan V.}",
note = "Funding Information: Funding: The experiments were implemented using the equipment and unique scientific installation “Biological collection–Genetic biomodels of neuropsychiatric disorders” (No. 493387) of the Federal State Budgetary Scientific Institution “Scientific Research Institute of Neurosciences and Medicine” theme no. AAAA-A21-121011990039-2 (2021–2025). The study partially used the facilities and equipment of the Resource Fund of Applied Genetics MIPT (support grant 075-15-2021-684). Publisher Copyright: {\textcopyright} 2022 by the authors. Licensee MDPI, Basel, Switzerland.",
year = "2022",
month = feb,
doi = "10.3390/toxics10020069",
language = "English",
volume = "10",
journal = "Toxics",
issn = "2305-6304",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
number = "2",

}

RIS

TY - JOUR

T1 - MPTP-Treated Zebrafish Recapitulate ‘Late-Stage’ Parkinson’s-like Cognitive Decline

AU - Bashirzade, Alim A.O.

AU - Cheresiz, Sergey V.

AU - Belova, Alisa S.

AU - Drobkov, Alexey V.

AU - Korotaeva, Anastasiia D.

AU - Azizi-Arani, Soheil

AU - Azimirad, Amirhossein

AU - Odle, Eric

AU - Gild, Emma Yanina V.

AU - Ardashov, Oleg V.

AU - Volcho, Konstantin P.

AU - Bozhko, Dmitrii V.

AU - Myrov, Vladislav O.

AU - Kolchanova, Sofia M.

AU - Polovian, Aleksander I.

AU - Galumov, Georgii K.

AU - Salakhutdinov, Nariman F.

AU - Amstislavskaya, Tamara G.

AU - Kalueff, Allan V.

N1 - Funding Information: Funding: The experiments were implemented using the equipment and unique scientific installation “Biological collection–Genetic biomodels of neuropsychiatric disorders” (No. 493387) of the Federal State Budgetary Scientific Institution “Scientific Research Institute of Neurosciences and Medicine” theme no. AAAA-A21-121011990039-2 (2021–2025). The study partially used the facilities and equipment of the Resource Fund of Applied Genetics MIPT (support grant 075-15-2021-684). Publisher Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland.

PY - 2022/2

Y1 - 2022/2

N2 - The zebrafish is a promising model species in biomedical research, including neurotoxicology and neuroactive drug screening. 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) evokes degeneration of dopaminergic neurons and is commonly used to model Parkinson’s disease (PD) in laboratory animals, including zebrafish. However, cognitive phenotypes in MPTP-evoked experimental PD models remain poorly understood. Here, we established an LD50 (292 mg/kg) for intraperitoneal MPTP administration in adult zebrafish, and report impaired spatial working memory (poorer spontaneous alternation in the Y-maze) in a PD model utilizing fish treated with 200 µg of this agent. In addition to conventional behavioral analyses, we also employed artificial intelligence (AI)-based approaches to independently and without bias characterize MPTP effects on zebrafish behavior during the Y-maze test. These analyses yielded a distinct cluster for 200-µg MPTP (vs. other) groups, suggesting that high-dose MPTP produced distinct, computationally detectable patterns of zebrafish swimming. Collectively, these findings support MPTP treatment in adult zebrafish as a late-stage experimental PD model with overt cognitive phenotypes.

AB - The zebrafish is a promising model species in biomedical research, including neurotoxicology and neuroactive drug screening. 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) evokes degeneration of dopaminergic neurons and is commonly used to model Parkinson’s disease (PD) in laboratory animals, including zebrafish. However, cognitive phenotypes in MPTP-evoked experimental PD models remain poorly understood. Here, we established an LD50 (292 mg/kg) for intraperitoneal MPTP administration in adult zebrafish, and report impaired spatial working memory (poorer spontaneous alternation in the Y-maze) in a PD model utilizing fish treated with 200 µg of this agent. In addition to conventional behavioral analyses, we also employed artificial intelligence (AI)-based approaches to independently and without bias characterize MPTP effects on zebrafish behavior during the Y-maze test. These analyses yielded a distinct cluster for 200-µg MPTP (vs. other) groups, suggesting that high-dose MPTP produced distinct, computationally detectable patterns of zebrafish swimming. Collectively, these findings support MPTP treatment in adult zebrafish as a late-stage experimental PD model with overt cognitive phenotypes.

KW - 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)

KW - Artificial intelligence

KW - Inhibitory avoidance task

KW - Parkinson’s disease

KW - Spontaneous alternation

KW - Zebrafish

UR - http://www.scopus.com/inward/record.url?scp=85124333068&partnerID=8YFLogxK

U2 - 10.3390/toxics10020069

DO - 10.3390/toxics10020069

M3 - Article

C2 - 35202255

AN - SCOPUS:85124333068

VL - 10

JO - Toxics

JF - Toxics

SN - 2305-6304

IS - 2

M1 - 69

ER -

ID: 35538791