TY - JOUR

T1 - Morphological variability and genetic structure of Miscanthus sinensis (Poaceae) cultivated in the forest-steppe of Western Siberia

AU - Dorogina, O. V.

AU - Kuban, I. N.

AU - Zueva, G. A.

AU - Zhmud, E. V.

AU - Vasilyeva, O. Y.

N1 - Dorogina O.V., Kuban I.N., Zueva G.A., Zhmud E.V., Vasilyeva O.Yu. Morphological variability and genetic structure of Miscanthus sinensis (Poaceae) cultivated in the forest-steppe of Western Siberia. Vavilovskii Zhurnal Genetiki i Selektsii=Vavilov J Genet Breed. 2025;29(2):210-218. doi 10.18699/vjgb-25-24 This study was conducted within the framework of state assignments for Central Siberian Botanical Garden SB RAS (project “Analysis of biodiversity, conservation and restoration of rare and resource plant species using experimental methods”, No. AAAA-A21-121011290025-2) and with the financial support of the Ministry of Science and Higher Education of the Russian Federation No. FSUS-2024-0024.

PY - 2025

Y1 - 2025

N2 - Miscanthus sinensis Andersson (Poaceae) grows in monsoon climate. For this reason, when cultured under the conditions of a short growing season of Western Siberia, full-fledged seeds do not have time to form. We have studied a large number of specimens of this species from Primorsky Krai in the collection of the Central Siberian Botanical Garden, SB RAS. Using these samples, it was possible for the first time to select forms that produce high-quality mature seeds in local conditions during a short growing season, possibly due to spontaneous hybridization of early flowering forms. We obtained the first and second (G1 and G2) generations from these seeds and checked for hybrids. The aim of this study is selection, biomorphological characterization of early flowering ornamental forms of M. sinensis and analysis of genetic polymorphism of the selected forms (S1, S2) and the obtained G1 and G2 generations using ISSR markers. Under the conditions of introduction, the selected samples of M. sinensis were characterized by complex resistance, high decorativeness, reached the ontogenetic state of mature generative plants and differed from other samples in the collection by early flowering and the formation of full-fledged seeds. Thus, the forms of M. sinensis we selected are promising for landscape design and breeding. When studying the genetic structure of G1, G2 and two generations of the sample using ISSR markers, three effective stable unique PCR fragments were identified. A study of the genetic variability of the resulting G1 generation showed complete uniformity of genotypes. In the G2 generation, variability was observed, and we found five sets of genotypes, which were also confirmed in the dendrogram. As a result, unique molecular polymorphic fragments were identified. Their length was 300–3000 bp, and the genetic formula for certification of M. sinensis was compiled.

AB - Miscanthus sinensis Andersson (Poaceae) grows in monsoon climate. For this reason, when cultured under the conditions of a short growing season of Western Siberia, full-fledged seeds do not have time to form. We have studied a large number of specimens of this species from Primorsky Krai in the collection of the Central Siberian Botanical Garden, SB RAS. Using these samples, it was possible for the first time to select forms that produce high-quality mature seeds in local conditions during a short growing season, possibly due to spontaneous hybridization of early flowering forms. We obtained the first and second (G1 and G2) generations from these seeds and checked for hybrids. The aim of this study is selection, biomorphological characterization of early flowering ornamental forms of M. sinensis and analysis of genetic polymorphism of the selected forms (S1, S2) and the obtained G1 and G2 generations using ISSR markers. Under the conditions of introduction, the selected samples of M. sinensis were characterized by complex resistance, high decorativeness, reached the ontogenetic state of mature generative plants and differed from other samples in the collection by early flowering and the formation of full-fledged seeds. Thus, the forms of M. sinensis we selected are promising for landscape design and breeding. When studying the genetic structure of G1, G2 and two generations of the sample using ISSR markers, three effective stable unique PCR fragments were identified. A study of the genetic variability of the resulting G1 generation showed complete uniformity of genotypes. In the G2 generation, variability was observed, and we found five sets of genotypes, which were also confirmed in the dendrogram. As a result, unique molecular polymorphic fragments were identified. Their length was 300–3000 bp, and the genetic formula for certification of M. sinensis was compiled.

KW - G1 and G2 generations

KW - ISSR markers

KW - S1 and S2 selected forms

KW - certification

KW - decorative form of Miscanthus sinensis

KW - quality of seeds

UR - https://www.scopus.com/pages/publications/105003765766

UR - https://elibrary.ru/item.asp?id=80606690

UR - https://www.mendeley.com/catalogue/b443dcc6-dd2a-3252-86a3-bb5c8abc650f/

U2 - 10.18699/vjgb-25-24

DO - 10.18699/vjgb-25-24

M3 - Article

C2 - 40264809

VL - 29

SP - 210

EP - 218

JO - Вавиловский журнал генетики и селекции

JF - Вавиловский журнал генетики и селекции

SN - 2500-0462

IS - 2

M1 - 4

ER -