Standard

Molecular sexing of Lepidoptera. / Belousova, Irina; Ershov, Nikita; Pavlushin, Sergey et al.

In: Journal of Insect Physiology, Vol. 114, 01.04.2019, p. 53-56.

Research output: Contribution to journal › Article › peer-review

Harvard

Belousova, I, Ershov, N, Pavlushin, S, Ilinsky, Y & Martemyanov, V 2019, 'Molecular sexing of Lepidoptera', Journal of Insect Physiology, vol. 114, pp. 53-56. https://doi.org/10.1016/j.jinsphys.2019.02.005

APA

Belousova, I., Ershov, N., Pavlushin, S., Ilinsky, Y., & Martemyanov, V. (2019). Molecular sexing of Lepidoptera. Journal of Insect Physiology, 114, 53-56. https://doi.org/10.1016/j.jinsphys.2019.02.005

Vancouver

Belousova I, Ershov N, Pavlushin S, Ilinsky Y, Martemyanov V. Molecular sexing of Lepidoptera. Journal of Insect Physiology. 2019 Apr 1;114:53-56. doi: 10.1016/j.jinsphys.2019.02.005

Author

Belousova, Irina ; Ershov, Nikita ; Pavlushin, Sergey et al. / Molecular sexing of Lepidoptera. In: Journal of Insect Physiology. 2019 ; Vol. 114. pp. 53-56.

BibTeX

@article{a58d6385e0ac40039c00bd8538b6a4b3,
title = "Molecular sexing of Lepidoptera",
abstract = "We developed a universal method of Lepidoptera molecular sexing. The method is based on comparing the number of copies of the same gene in different sexes. Males of the majority of lepidopteran species have two Z chromosomes, whereas females have only one Z chromosome. Correspondingly, the number of copies of each gene located on this chromosome differs by two times between males and females. For quantitative estimation, we used qPCR. Via multiple alignment of the kettin (a Z chromosome gene) nucleotide sequences, we detected the most conserved fragment and designed primers with broad interspecies specificity for Lepidoptera. Using these primers, we successfully determined the sex of three lepidopteran species belonging to different superfamilies. The developed method is a simple, cost-effective and high-throughput technique for routine sexing. The sex of lepidopteran individuals can be examined at any developmental stage.",
keywords = "Galleria mellonella, Kettin, Lymantria dispar, Plutella xylostella, Sex, Z chromosome",
author = "Irina Belousova and Nikita Ershov and Sergey Pavlushin and Yury Ilinsky and Vyacheslav Martemyanov",
note = "Publisher Copyright: {\textcopyright} 2019 Elsevier Ltd",
year = "2019",
month = apr,
day = "1",
doi = "10.1016/j.jinsphys.2019.02.005",
language = "English",
volume = "114",
pages = "53--56",
journal = "Journal of Insect Physiology",
issn = "0022-1910",
publisher = "Elsevier Ltd",

}

RIS

TY - JOUR

T1 - Molecular sexing of Lepidoptera

AU - Belousova, Irina

AU - Ershov, Nikita

AU - Pavlushin, Sergey

AU - Ilinsky, Yury

AU - Martemyanov, Vyacheslav

N1 - Publisher Copyright: © 2019 Elsevier Ltd

PY - 2019/4/1

Y1 - 2019/4/1

N2 - We developed a universal method of Lepidoptera molecular sexing. The method is based on comparing the number of copies of the same gene in different sexes. Males of the majority of lepidopteran species have two Z chromosomes, whereas females have only one Z chromosome. Correspondingly, the number of copies of each gene located on this chromosome differs by two times between males and females. For quantitative estimation, we used qPCR. Via multiple alignment of the kettin (a Z chromosome gene) nucleotide sequences, we detected the most conserved fragment and designed primers with broad interspecies specificity for Lepidoptera. Using these primers, we successfully determined the sex of three lepidopteran species belonging to different superfamilies. The developed method is a simple, cost-effective and high-throughput technique for routine sexing. The sex of lepidopteran individuals can be examined at any developmental stage.

AB - We developed a universal method of Lepidoptera molecular sexing. The method is based on comparing the number of copies of the same gene in different sexes. Males of the majority of lepidopteran species have two Z chromosomes, whereas females have only one Z chromosome. Correspondingly, the number of copies of each gene located on this chromosome differs by two times between males and females. For quantitative estimation, we used qPCR. Via multiple alignment of the kettin (a Z chromosome gene) nucleotide sequences, we detected the most conserved fragment and designed primers with broad interspecies specificity for Lepidoptera. Using these primers, we successfully determined the sex of three lepidopteran species belonging to different superfamilies. The developed method is a simple, cost-effective and high-throughput technique for routine sexing. The sex of lepidopteran individuals can be examined at any developmental stage.

KW - Galleria mellonella

KW - Kettin

KW - Lymantria dispar

KW - Plutella xylostella

KW - Sex

KW - Z chromosome

UR - http://www.scopus.com/inward/record.url?scp=85062476525&partnerID=8YFLogxK

U2 - 10.1016/j.jinsphys.2019.02.005

DO - 10.1016/j.jinsphys.2019.02.005

M3 - Article

C2 - 30776424

AN - SCOPUS:85062476525

VL - 114

SP - 53

EP - 56

JO - Journal of Insect Physiology

JF - Journal of Insect Physiology

SN - 0022-1910

ER -

ID: 18679879