TY - JOUR

T1 - Molecular mechanisms underlying the impact of mutations in SOD1 on its conformational properties associated with amyotrophic lateral sclerosis as revealed with molecular modelling

AU - Alemasov, Nikolay A.

AU - Ivanisenko, Nikita V.

AU - Ramachandran, Srinivasan

AU - Ivanisenko, Vladimir A.

N1 - Publisher Copyright: © 2018 The Author(s).

PY - 2018/2/5

Y1 - 2018/2/5

N2 - Background: So far, little is known about the molecular mechanisms of amyotrophic lateral sclerosis onset and progression caused by SOD1 mutations. One of the hypotheses is based on SOD1 misfolding resulting from mutations and subsequent deposition of its cytotoxic aggregates. This hypothesis is complicated by the fact that known SOD1 mutations of similar clinical effect could be distributed over the whole protein structure. Results: In this work, a measure of hydrogen bond stability in conformational states was studied with elastic network analysis of 35 SOD1 mutants. Twenty-eight hydrogen bonds were detected in nine of 35 mutants with their stability being significantly different from that with the wild-type. These hydrogen bonds were formed by the amino acid residues known from the literature to be located in contact between SOD1 aggregates. Additionally, residues disposed between copper binding sites of both protein subunits were found from the models to form a stiff core, which can be involved in mechanical impulse transduction between these active centres. Conclusions: The modelling highlights that both stability of the copper binding site and stability of the dimer can play an important role in ALS progression.

AB - Background: So far, little is known about the molecular mechanisms of amyotrophic lateral sclerosis onset and progression caused by SOD1 mutations. One of the hypotheses is based on SOD1 misfolding resulting from mutations and subsequent deposition of its cytotoxic aggregates. This hypothesis is complicated by the fact that known SOD1 mutations of similar clinical effect could be distributed over the whole protein structure. Results: In this work, a measure of hydrogen bond stability in conformational states was studied with elastic network analysis of 35 SOD1 mutants. Twenty-eight hydrogen bonds were detected in nine of 35 mutants with their stability being significantly different from that with the wild-type. These hydrogen bonds were formed by the amino acid residues known from the literature to be located in contact between SOD1 aggregates. Additionally, residues disposed between copper binding sites of both protein subunits were found from the models to form a stiff core, which can be involved in mechanical impulse transduction between these active centres. Conclusions: The modelling highlights that both stability of the copper binding site and stability of the dimer can play an important role in ALS progression.

KW - Aggregates

KW - ALS

KW - Copper

KW - Elastic networks

KW - Hydrogen bonds

KW - Misfolding

KW - SOD1

KW - Protein Structure, Secondary

KW - Mutation/genetics

KW - Humans

KW - Models, Molecular

KW - Amyotrophic Lateral Sclerosis/enzymology

KW - Hydrogen Bonding

KW - Superoxide Dismutase-1/chemistry

KW - Survival Analysis

KW - Conserved Sequence

KW - Protein Conformation

KW - Amino Acids/genetics

KW - Evolution, Molecular

KW - PROTEIN

KW - COPPER

KW - ELASTIC NETWORK MODELS

KW - MUTANTS

KW - THERMAL-STABILITY

KW - FLEXIBILITY

KW - HYDROGEN-BONDS

KW - ANISOTROPIC RESPONSE

KW - ZN SUPEROXIDE-DISMUTASE

UR - http://www.scopus.com/inward/record.url?scp=85041416090&partnerID=8YFLogxK

U2 - 10.1186/s12900-018-0080-9

DO - 10.1186/s12900-018-0080-9

M3 - Article

C2 - 29431095

AN - SCOPUS:85041416090

VL - 18

SP - 1

JO - BMC Structural Biology

JF - BMC Structural Biology

SN - 1472-6807

IS - Suppl 1

M1 - 1

ER -