Research output: Contribution to journal › Article › peer-review
Modified Oligonucleotides for Guiding RNA Cleavage Using Bacterial RNase P. / Novopashina, D. S.; Nazarov, A. S.; Vorobjeva, M. A. et al.
In: Molecular Biology, Vol. 52, No. 6, 01.11.2018, p. 905-912.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Modified Oligonucleotides for Guiding RNA Cleavage Using Bacterial RNase P
AU - Novopashina, D. S.
AU - Nazarov, A. S.
AU - Vorobjeva, M. A.
AU - Kuprushkin, M. S.
AU - Davydova, A. S.
AU - Lomzov, A. A.
AU - Pyshnyi, D. V.
AU - Altman, S.
AU - Venyaminova, A. G.
PY - 2018/11/1
Y1 - 2018/11/1
N2 - Abstract: The ability of a series of novel modified external guide sequences (EGS oligonucleotides) to induce the hydrolysis of target RNA with bacterial ribonuclease P has been studied; the most efficient modification variants have been selected. We have found patterns of the oligonucleotide sugar-phosphate backbone modi-fications that enhance oligonucleotide stability in the biological environment and do not violate the ability to interact with the enzyme and induce the RNA hydrolysis. It has been shown that analogues of EGS oligonucleotides selectively modified at 2'-position (2'-O-methyl and 2'-fluoro) or at internucleotide phosphates (phosphoryl guanidines) can be used for the addressed cleavage of a model RNA target by bacterial RNase P. The ability of new phosphoryl guanidine analogues of oligodeoxyribonucleotides that are stable in biological media to induce the hydrolysis of target RNA with bacterial ribonuclease P has been shown for the first time. The modified EGS oligonucleotides with an optimal balance between functional activity and stability in biological media can be considered as potential antibacterial agents.
AB - Abstract: The ability of a series of novel modified external guide sequences (EGS oligonucleotides) to induce the hydrolysis of target RNA with bacterial ribonuclease P has been studied; the most efficient modification variants have been selected. We have found patterns of the oligonucleotide sugar-phosphate backbone modi-fications that enhance oligonucleotide stability in the biological environment and do not violate the ability to interact with the enzyme and induce the RNA hydrolysis. It has been shown that analogues of EGS oligonucleotides selectively modified at 2'-position (2'-O-methyl and 2'-fluoro) or at internucleotide phosphates (phosphoryl guanidines) can be used for the addressed cleavage of a model RNA target by bacterial RNase P. The ability of new phosphoryl guanidine analogues of oligodeoxyribonucleotides that are stable in biological media to induce the hydrolysis of target RNA with bacterial ribonuclease P has been shown for the first time. The modified EGS oligonucleotides with an optimal balance between functional activity and stability in biological media can be considered as potential antibacterial agents.
KW - 2'-fluoro modified oligoribonucleotides
KW - bacterial RNase P
KW - EGS oligonucleotides
KW - modified oligonucleotides
KW - oligo(2'-О-methylribonucleotides)
KW - phosphoryl guanidine oligonucleotides
KW - SEQUENCES
KW - RIBONUCLEASE-P
KW - PEPTIDE
KW - NUCLEIC-ACIDS
KW - INHIBITION
KW - MORPHOLINO OLIGOMER CONJUGATE
KW - RESISTANCE
KW - oligo(2'-?-methylribonucleotides)
KW - SUBUNIT
UR - http://www.scopus.com/inward/record.url?scp=85059342255&partnerID=8YFLogxK
U2 - 10.1134/S0026893318060134
DO - 10.1134/S0026893318060134
M3 - Article
AN - SCOPUS:85059342255
VL - 52
SP - 905
EP - 912
JO - Molecular Biology
JF - Molecular Biology
SN - 0026-8933
IS - 6
ER -
ID: 18073820