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Modified multiplex real-time PCR for quantification of differently sized cell-free dna fragments. / Sokolova, E. A.; Khlistun, I. V.; Kushlinsky, D. N.

In: Bulletin of Russian State Medical University, Vol. 6, No. 4, 01.07.2017, p. 19-23.

Research output: Contribution to journal › Article › peer-review

Harvard

Sokolova, EA, Khlistun, IV & Kushlinsky, DN 2017, 'Modified multiplex real-time PCR for quantification of differently sized cell-free dna fragments', Bulletin of Russian State Medical University, vol. 6, no. 4, pp. 19-23. https://doi.org/10.24075/brsmu.2017-04-03

APA

Sokolova, E. A., Khlistun, I. V., & Kushlinsky, D. N. (2017). Modified multiplex real-time PCR for quantification of differently sized cell-free dna fragments. Bulletin of Russian State Medical University, 6(4), 19-23. https://doi.org/10.24075/brsmu.2017-04-03

Vancouver

Sokolova EA, Khlistun IV, Kushlinsky DN. Modified multiplex real-time PCR for quantification of differently sized cell-free dna fragments. Bulletin of Russian State Medical University. 2017 Jul 1;6(4):19-23. doi: 10.24075/brsmu.2017-04-03

Author

Sokolova, E. A. ; Khlistun, I. V. ; Kushlinsky, D. N. / Modified multiplex real-time PCR for quantification of differently sized cell-free dna fragments. In: Bulletin of Russian State Medical University. 2017 ; Vol. 6, No. 4. pp. 19-23.

BibTeX

@article{9034a889189340f69765c527897dcbf9,
title = "Modified multiplex real-time PCR for quantification of differently sized cell-free dna fragments",
abstract = "There is evidence that size distribution of cell-free DNA (cfDNA) fragments can be diagnostically relevant. The present work describes a multiplex quantitative real-time polymerase chain reaction technique modified and validated by the authors to study the degree of cfDNA fragmentation in blood plasma. Based on the detection of Alu and hLINE-1 repeats, this technique employs fluorescent probes. We selected suitable primers and probes, optimized PCR conditions and estimated the dynamic range and sensitivity threshold of the assay. The modified PCR had a dynamic range of 6 logs, its efficiency being over 90 %. We demonstrated that cfDNA fragmentation index did not differ significantly between healthy women (n = 16) and women with stage III–IV ovarian cancer (n = 14). Therefore, further research on a larger sample is needed using electrophoretic cfDNA fractionation.",
keywords = "Alu repeats, Cell-free DNA, CfDNA, Fluorescent probe, HLINE-1 repeats, Multiplex quantitative PCR, Primers",
author = "Sokolova, {E. A.} and Khlistun, {I. V.} and Kushlinsky, {D. N.}",
year = "2017",
month = jul,
day = "1",
doi = "10.24075/brsmu.2017-04-03",
language = "English",
volume = "6",
pages = "19--23",
journal = "Bulletin of Russian State Medical University",
issn = "2500-1094",
publisher = "Pirogov Russian National Research Medical University",
number = "4",

}

RIS

TY - JOUR

T1 - Modified multiplex real-time PCR for quantification of differently sized cell-free dna fragments

AU - Sokolova, E. A.

AU - Khlistun, I. V.

AU - Kushlinsky, D. N.

PY - 2017/7/1

Y1 - 2017/7/1

N2 - There is evidence that size distribution of cell-free DNA (cfDNA) fragments can be diagnostically relevant. The present work describes a multiplex quantitative real-time polymerase chain reaction technique modified and validated by the authors to study the degree of cfDNA fragmentation in blood plasma. Based on the detection of Alu and hLINE-1 repeats, this technique employs fluorescent probes. We selected suitable primers and probes, optimized PCR conditions and estimated the dynamic range and sensitivity threshold of the assay. The modified PCR had a dynamic range of 6 logs, its efficiency being over 90 %. We demonstrated that cfDNA fragmentation index did not differ significantly between healthy women (n = 16) and women with stage III–IV ovarian cancer (n = 14). Therefore, further research on a larger sample is needed using electrophoretic cfDNA fractionation.

AB - There is evidence that size distribution of cell-free DNA (cfDNA) fragments can be diagnostically relevant. The present work describes a multiplex quantitative real-time polymerase chain reaction technique modified and validated by the authors to study the degree of cfDNA fragmentation in blood plasma. Based on the detection of Alu and hLINE-1 repeats, this technique employs fluorescent probes. We selected suitable primers and probes, optimized PCR conditions and estimated the dynamic range and sensitivity threshold of the assay. The modified PCR had a dynamic range of 6 logs, its efficiency being over 90 %. We demonstrated that cfDNA fragmentation index did not differ significantly between healthy women (n = 16) and women with stage III–IV ovarian cancer (n = 14). Therefore, further research on a larger sample is needed using electrophoretic cfDNA fractionation.

KW - Alu repeats

KW - Cell-free DNA

KW - CfDNA

KW - Fluorescent probe

KW - HLINE-1 repeats

KW - Multiplex quantitative PCR

KW - Primers

UR - http://www.scopus.com/inward/record.url?scp=85032811118&partnerID=8YFLogxK

U2 - 10.24075/brsmu.2017-04-03

DO - 10.24075/brsmu.2017-04-03

M3 - Article

AN - SCOPUS:85032811118

VL - 6

SP - 19

EP - 23

JO - Bulletin of Russian State Medical University

JF - Bulletin of Russian State Medical University

SN - 2500-1094

IS - 4

ER -

ID: 10066798